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Giemsa Stain: Preparation, Procedure, Principle, Composition and Application

Giemsa Stain: Staining Procedure, Principle, Result and Application

Advertisements The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain with a combination of reagents to detect the presence of malaria parasites. This stain is used for nucleic acid staining and histopathological diagnosis of malaria and other parasites. Giemsa Stain is a types of Romanowsky … Read more

Nitrate Reduction Test Procedure, Principle, Result

Nitrate Reduction Test Procedure, Principle, Result

What is Nitrate Reduction Test? Nitrate reduction test is used to differentiate Enterobacteriaceae based on their ability to produce nitrate reductase enzyme, which hydrolyzes nitrate (NO3–) to nitrite (NO2–), which may be further degraded to various nitrogen products such as nitrogen oxide, nitrous oxide, and ammonia (NH3), depending on the organism’s enzyme system and the … Read more

Tollens’ Test Definition, Principle, Procedure, Result, Application.

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Oxidase Test Definition, Principle, Procedure, Result, Application

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Gelatin Hydrolysis Test Purpose, Principle, Procedure, Result.

Gelatin Hydrolysis Test Purpose, Principle, Procedure, Result.

The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.

Pour Plate Method Principle, Procedure, Objective, Result, Advantages

Pour Plate Method Principle, Procedure, Objective, Result

Advertisements The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be … Read more

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread Plate Method in microbiology Advertisements The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread … Read more

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

Advertisements Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present within the membrane-bounded structure known as the nucleus. In case of prokaryotes the nuclear material is present within the nucleoid which lacks the nuclear membrane and does not follow the mitosis or miosis cell division. … Read more

Cell Membrane Staining Procedure, Principle, Result

BACTERIAL CELL MEMBRANE STAINING

Advertisements The cell membrane is a biological membrane which also termed a plasma membrane (PM) or cytoplasmic membrane. It divides the interior of the cell from the outside environment, thus protects the cell from environmental stress. The cell membrane is a lipid bilayer, contains cholesterols between phospholipids which maintain their fluidity at various temperatures. The … Read more

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