Western Blot Protocol, Principle, Result
MN Editors
In
Protocols

Western Blot Protocol, Principle, Result.

In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.

Northern Blot Protocol, Principle, Application, Result
MN Editors
In
Protocols

Northern Blotting Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Widal Test Principle, Procedure, Result, Advantages, Limitation
MN Editors
In
Laboratory Tests

Widal Test – Principle, Procedure, Result, Advantages, Limitation

The Widal test is an agglutination test that determines any antibodies present in the patient's serum that are generated against the causal agents of the enteric febrile illness (Salmonella Typhi, and Salmonella paratyphi B, A as well as C).

Tollens’ Test Definition, Principle, Procedure, Result, Application.
MN Editors
In
Biochemical Test

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Oxidase Test Definition, Principle, Procedure, Result, Application
MN Editors
In
Biochemical Test

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Coagulase Test Principle, Procedure, Application, Result.
MN Editors
In
Microbiology Techniques

Coagulase Test Principle, Procedure, Application, Result.

It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).

Gelatin Hydrolysis Test Purpose, Principle, Procedure, Result.
MN Editors
In
Biochemical Test

Gelatin Hydrolysis Test Purpose, Principle, Procedure, Result.

The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.

Reverse CAMP test
MN Editors
In
Biochemical Test

Reverse CAMP test Principle, Procedure, Result

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Restriction Digest Principle, Procedure, result
MN Editors
In
Protocols

Restriction Digest Protocol, Principle, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.