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Physiological Buffer preparation

Buffer preparation

Tyrode’s Solution, Acidic Preparation Advertisements To prepare 1L of Tyrode’s Solution, Acidic, the following components are required; Component Amount Concentration NaCl (mw: 58.44 g/mol) 8 g 0.1368 M KCl (mw: 74.55 g/mol) 200 mg 0.0027 M CaCl2•2H2O (mw: 147.01 g/mol) 240 mg 0.0016 M MgCl2•6H2O (mw: 203.3 g/mol) 100 mg 0.0005 M Glucose (mw: 180.16 … Read more

Different Buffer preparation for Gel Electrophoresis

Buffer preparation

Acrylamide Stock Solution Preparation Advertisements Application: Acrylamide is used along with bisacrylamide to form gels used in SDS-PAGE. Acrylamide forms polyacrylamide polymers, while bisacrylamide crosslinks between polyacrylamide chains. To prepare 1L of Acrylamide Stock Solution the following components are required; Component Amount Concentration acrylamide (mw: 71.08 g/mol) 389.6 g 5.481 M bisacrylamide (mw: 154.17 g/mol) … Read more

Solution and Cell/Culture/Growth Media Preparations

Buffer preparation

Chelation Medium Preparation Advertisements To prepare 1L of Chelation Medium the following components are required. Component Amount Concentration NaCl (mw: 58.44 g/mol) 40.9 g 0.7 M MgCl2·6H2O (mw: 203.3 g/mol) 6.1 g 0.03 M MgSO4·7H2O (mw: 246.47 g/mol) 7.39 g 0.03 M KCl (mw: 74.55 g/mol) 1.49 g 0.02 M EGTA (mw: 380.35 g/mol) 7.6 … Read more

Carbenicillin Solution Preparation

Buffer preparation

Advertisements Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against Gram-positive bacteria. Carbenicillin binds and inhibits the enzymes involved in the synthesis of bacterial cell wall. Since carbenicillin is less sensitive to β-lactamases than ampicillin, it is effective against ampicillin-resistant bacteria. It … Read more

Calcium Chloride Stock Solution Preparation

Buffer preparation

Calcium Chloride Stock Solution Preparation Advertisements Calcium chloride is a common calcium salt used in a variety of applications. To prepare 1L of Calcium Chloride Stock Solution the following components are required. Component Amount Concentration CaCl2 (mw: 110.98 g/mol) 58 g 0.5226 M Preparation procedure Prepare 800 mL of distilled water in a suitable container. … Read more

Bath Solution (Ca2+/Mg2+ Free) Preparation

Buffer preparation

Bath Solution Preparation Advertisements To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required; Component Amount Concentration KOH (mw: 56.11 g/mol) 7.854 g 0.14 M HEPES (mw: 238.3 g/mol) 4.766 g 0.02 M KCl (mw: 74.55 g/mol) 149 mg 0.002 M EGTA (mw: 380.35 g/mol) 1.902 g 0.005 M Methanesulfonic acid (mw: … Read more

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