A Spheroplast (or sphaeroplast according to British use) can be described as a microbe organism with a cell wall that is almost entirely gone through the penicillin or Lysozyme. According to certain definitions the term is employed to refer to Gram-negative bacteria. In other definitions, the word also includes yeasts. Spheroplast’s name is derived in the sense that once the cell wall of the microbe is digested by membrane tension, the cell to take on the characteristic shape of a spherical. Spheroplasts can be osmotically fragile and can lyse when transferred to a hypotonic environment.