Reverse transfection is a method of introducing genetic material into cells without the use of a transfection reagent.
It is also known as cell-to-vector transfection or reverse transduction.
Reverse transfection is typically used in vitro to introduce plasmid DNA into cells.
The method involves the use of genetically modified cells, which produce and secrete a recombinant virus carrying the desired genetic material.
The virus infects neighboring cells and introduces the plasmid DNA into them.
This method is used to study gene expression and function, as well as to produce recombinant proteins.
Reverse transfection is a versatile technique, as it can be used with a variety of cell types, including primary cells and stem cells.
It is a relatively quick and simple process, with high efficiency and low toxicity.
Reverse transfection is often used as an alternative to traditional transfection methods, which can be challenging and time-consuming.
There are several advantages to using reverse transfection, including the ability to produce stable cell lines, the ability to study gene function in a controlled manner, and the ability to produce large amounts of recombinant protein.