Biochemical Test and Identification of different microorganisms

Biochemical Test and Identification

  • Biochemical tests are the kinds of tests that are done on different bacteria to figure out what kind of bacteria they are based on how they react to different biochemical compounds.
  • Biochemical tests, which are usually done with phenotypic identification, are one of the traditional ways to figure out what kind of microorganism it is.
  • These methods were widely used for a long time, and they are still used today, especially in some lab procedures where a certain type of microorganism needs to be identified quickly.
  • Several biochemical tests are based on the ability of microorganisms to use certain biomolecules to make organic compounds that they can use.
  • There are different kinds of biochemical tests, and different ways to tell the difference between different microorganisms.
  • One of the older methods is to look for an increase in turbidity in the liquid medium. This shows that the organism is growing and getting the nutrients it needs.
  • In other tests, on the other hand, the results are based on how the colour of the medium changes as its pH changes.
  • Microorganisms can be put into different groups based on how they respond to these kinds of tests. Some tests can even tell the difference between different species of microorganisms.
  • Because of this, biochemical tests are important because they are cheap and easy to do.
  • The way bacteria and other microorganisms work is different from one another, which makes it possible to tell them apart.
  • Biochemical tests, on the other hand, have some flaws. Even though these methods are cheap and can give both quantitative and qualitative information about the variety of microorganisms in a sample, they are difficult to use and take a long time to see results.
  • Sometimes, false positives are found, especially when similar microbial species are being tested.
What is the purpose of biochemical test? Biochemical tests are used to tell the difference between different types of bacteria based on how they behave in the biochemical world. What are examples of biochemical tests?
  • Catalase Test.
  • Mannitol Salt Agar (MSA)
  • Blood Agar Plates (BAP)
  • Streak-stab technique.
  • Taxos P (optochin sensitivity testing)
  • Taxos A (bacitracin sensitivity testing)
  • CAMP Test.
  • Bile Esculin Agar.

Biochemical Tests of Streptococcus mutans Results
CapsuleCapsulated
CatalaseNegative (-ve)
Gram StainingPositive (+ve)
HemolysisAlfa Hemolysis
MotilityNon-motile
OF (Oxidative-Fermentative)Facultative anaerobes
OxidaseNegative (-ve)
ShapeCocci
SporeNon-sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArbutinPositive (+ve)
CellobiosePositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
GlycogenNegative (-ve)
HippurateNegative (-ve)
InositolNegative (-ve)
InulinPositive (+ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseVariable
RaffinosePositive (+ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinPositive (+ve)
SorbitolPositive (+ve)
StarchNegative (-ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Alkaline PhosphataseNegative (-ve)
Arginine DehydrolaseNegative (-ve)
Esculin HydrolysisPositive (+ve)
HyalurodinaseNegative (-ve)
NeuraminidaseNegative (-ve)
Biochemical Tests of Salmonella Typhi Results
Capsule Test:Negative (-ve)
Catalase Test:Positive (+ve)
Citrate Test:Negative (-ve)
Flagella Test:Positive (+ve)
Gas Test:Negative (-ve)
Gelatin Hydrolysis Test:Negative (-ve)
Gram Staining Test:Negative (-ve)
Growth in KCN Test:Negative (-ve)
H2S Test:Positive (+ve)
Indole Test:Negative (-ve)
Motility Test:Motile
MR (Methyl Red) Test:Positive (+ve)
MUG TestNegative (-ve)
Nitrate Reduction Test:Positive (+ve)
Oxidase Test:Negative (-ve)
Pigment Test:Negative (-ve)
ShapeRod
Spore Test:Negative (-ve)
TSIA (Triple Sugar Iron Agar) Test:Alkali/Acid
Urease Test:Negative (-ve)
VP (Voges Proskauer) Test:Negative (-ve)
Adonitol Test:Negative (-ve)
Arabinose Test:Negative (-ve)
Arabitol Test:Negative (-ve)
Cellobiose Test:Negative (-ve)
DNase Test:Negative (-ve)
Dulcitol Test:Negative (-ve)
Erythritol Test:Negative (-ve)
Esculin Hydrolysis Test:Negative (-ve)
Glucose Test:Positive (+ve)
Glycerol Test:Negative (-ve)
Inositol Test:Negative (-ve)
Lactose Test:Negative (-ve)
Malonate Test:Negative (-ve)
Maltose Test:Positive (+ve)
Mannitol Test:Positive (+ve)
Mannose Test:Positive (+ve)
Melibiose Test:Positive (+ve)
Mucate Test:Negative (-ve)
MyoInositol Test:Negative (-ve)
Raffinose Test:Negative (-ve)
Rhamnose Test:Negative (-ve)
Salicin Test:Negative (-ve)
Sorbitol Test:Positive (+ve)
Sucrose Test:Negative (-ve)
Tartrate Test:Positive (+ve)
Trehalose Test:Positive (+ve)
Xylose Test:Positive (+ve)
Acetate Utilization Test:Negative (-ve)
Arginine Dehydrolase Test:Negative (-ve)
Esculin Hydrolysis Test:Negative (-ve)
Lipase Test:Negative (-ve)
Lysine Test:Positive (+ve)
ONPG (β-galactosidase) Test:Negative (-ve)
Ornithine Decarboxylase Test:Negative (-ve)
Peroxidase Test:Negative (-ve)
Tyrosine Hydrolysis Test:Negative (-ve)
Biochemical Tests of Staphylococcus aureusResults
CapsuleNon-Capsulated
CatalasePositive (+ve)
CitratePositive (+ve)
CoagulasePositive (+ve)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingPositive (+ve)
H2SNegative (-ve)
HemolysisPositive (+ve)- Beta
IndoleNegative (-ve)
MotilityNegative (-ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentMostly Positive (+ve)
PYRNegative (-ve)
ShapeCocci
SporeNon-Sporing
UreasePositive (+ve)
VP (Voges Proskauer)Positive (+ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNasePositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
RaffinoseNegative (-ve)
RibosePositive (+ve)
SalicinNegative (-ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetoin ProductionPositive (+ve)
Alkaline PhosphatasePositive (+ve)
Arginine DehydrolasePositive (+ve)
HyalurodinasePositive (+ve)
LipasePositive (+ve)
Ornithine DecarboxylaseNegative (-ve)
Biochemical tests of Bacillus subtilisResult
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaFlagellated
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram Positive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OxidaseVariable
PigmentNegative (-ve)
ShapeRods
SporePositive (+ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
CellobiosePositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactoseVariable
GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenPositive (+ve)
InositolPositive (+ve)
InulinVariable
LactoseVariable
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseVariable
RaffinosePositive (+ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinPositive (+ve)
SorbitolPositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Arginine DehydrolaseNegative (-ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LecithinaseNegative (-ve)
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Tyrosine HydrolysisNegative (-ve)
CharacteristicsPseudomonas aeruginosa
Gram StainingNegative
Shape (Cocci/Diplococci/Rods)Rods
Motility (Motile / Non-Motile)Motile (Unipolar)
Capsule (Capsulated/Non-Capsulated)Non-Capsulated
Spore (Sporing/Non-Sporing)Non-Sporing
Flagella (Flagellated/Non-Flagellated)Single Flagella
CatalasePositive (+ve)
OxidasePositive (+ve)
MRNegative (-ve)
VPNegative (-ve)
OF (Oxidative/Fermentative)Oxidative
IndoleNegative (-ve)
CitratePositive (+ve)
UreaseNegative (-ve)
Nitrate ReductionPositive (+ve)
H2SNegative (-ve)
GasPositive (+ve)- From Nitrate
PYR
CAMP
Gelatin HydrolysisPositive (+ve)
Niacin
CoagulaseNegative (-ve)
Hemolysis (Alfa/Beta/Gamma)
Nagler
String Test
PigmentPositive (+ve)
Bile Solubility
Cetrimide Test Positive (+ve)
Arabinose
DNase
Fructose
GlucoseNegative (-ve)
Hippurate
Inositol
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolPositive (+ve)
Mannose
MyoInositol
Raffinose
Ribose
SorbitolNegative (-ve)
SucroseNegative (-ve)
Acetate Utilization
Acetoin Production
Acid Phosphatase
Alkaline Phosphatase
Amidase
Arginine dehydrolasePositive (+ve)
Arylsulphatase
Beta Lactamase
Hyalurodinase
Lecithinase
LipasePositive (+ve)
LysineNegative (-ve)
Neuraminidase
ONPG Test
Ornithine decarboxylaseNegative (-ve)
Phenylalanine deaminase
Perooxidase
Tellurite
Tributyrin
CharacteristicsE. coli
Gram StainingNegative
Shape (Cocci/Diplococci/Rods)Rods
Motility (Motile / Non-Motile)Motile
Capsule (Capsulated/Non-Capsulated)Variable
Spore (Sporing/Non-Sporing)Non-Sporing
Flagella (Flagellated/Non-Flagellated)Flagellated
CatalasePositive (+ve)
OxidaseNegative (-ve)
MRPositive (+ve)
VPNegative (-ve)
OF (Oxidative/Fermentative)Fermentative
IndolePositive (+ve)
CitrateNegative (-ve)
UreaseNegative (-ve)
Nitrate ReductionPositive (+ve)
H2SNegative (-ve)
GasPositive (+ve)
PYR
CAMP
GelatinNegative (-ve)
Niacin
Coagulase
Hemolysis (Alfa/Beta/Gamma)Some Strains shows Hemolysis
Nagler
String Test
Pigment
Bile Solubility
ArabinosePositive (+ve)
DNaseNegative (-ve)
Fructose
GlucosePositive (+ve)
Hippurate
InositolNegative (+ve)
Inulin
LactosePositive (+ve)
Maltose
MannitolPositive (+ve)
Mannose
MyoInositol
Raffinose
Ribose
SorbitolPositive (+ve)
SucroseVariable
Acetate UtilizationPositive (+ve)
Acid Phosphatase
Alkaline Phosphatase
Amidase
Arginine dehydrolaseVariable
Arylsulphatase
Beta Lactamase
Hyalurodinase
Lecithinase
Lipase
LysinePositive (+ve)
Neuraminidase
ONPG TestPositive (+ve)
Ornithine decarboxylaseVariable
Phenylalanine deaminaseNegative (-ve)
Perooxidase
Tellurite
Tributyrin
Differential Antibiotic 
Novobiocin
Bacitracin
Optochin
Streptomysin
Biochemical Tests of Proteus mirabilisResults
CapsuleNegative (-ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
Gas from GlucosePositive (+ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingNegative (-ve)
Growth in KCNPositive (+ve)
H2SPositive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Facultative anaerobes
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporeNegative (-ve)
UreasePositive (+ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseNegative (-ve)
DNaseVariable
DulcitolNegative (-ve)
ErythritolNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
InositolNegative (-ve)
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
MucateNegative (-ve)
MyoInositolNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TartratePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Acetate UtilizationNegative (-ve)
Aesculine HydrolysisNegative (-ve)
Casein HydrolysisNegative (-ve)
Esculin HydrolysisNegative (-ve)
LipasePositive (+ve)
Lysine decarboxylasesNegative (-ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylasePositive (+ve)
Pectate DegradationNegative (-ve)
Phenylalanine DeaminasePositive (+ve)
Tryptophan DeaminaseNegative (-ve)
Biochemical Tests of Proteus mirabilisResults
CapsuleNegative (-ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
Gas from GlucosePositive (+ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingNegative (-ve)
Growth in KCNPositive (+ve)
H2SPositive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Facultative anaerobes
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporeNegative (-ve)
UreasePositive (+ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseNegative (-ve)
DNaseVariable
DulcitolNegative (-ve)
ErythritolNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
InositolNegative (-ve)
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
MucateNegative (-ve)
MyoInositolNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TartratePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Acetate UtilizationNegative (-ve)
Aesculine HydrolysisNegative (-ve)
Casein HydrolysisNegative (-ve)
Esculin HydrolysisNegative (-ve)
LipasePositive (+ve)
Lysine decarboxylasesNegative (-ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylasePositive (+ve)
Pectate DegradationNegative (-ve)
Phenylalanine DeaminasePositive (+ve)
Tryptophan DeaminaseNegative (-ve)
Biochemical TestsResults
CatalasePositive (+ve)
CitratePositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingPositive (+ve)
Growth in KCNPositive (+ve)
HemolysisPositive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionVariable
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporePositive (+ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseVariable
FructosePositive (+ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenPositive (+ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinVariable
SorbitolNegative (-ve)
StarchPositive (+ve)
SucroseVariable
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetate UtilizationVariable
Arginine DehydrolaseVariable
Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LecithinasePositive (+ve)
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Tyrosine HydrolysisPositive (+ve)
Biochemical Tests of Serratia marcescensResults
CapsuleNegative (-ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
GasVariable
Gelatin HydrolysisPositive (+ve)
Gram StainingNegative (-ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Facultative Anaerobes
OxidaseNegative (-ve)
PigmentPositive (+ve)
ShapeRods
SporeNegative (-ve)
UreasePositive (+ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolPositive (+ve)
ArabinoseNegative (-ve)
ArabitolVariable
CellobioseNegative (-ve)
DNasePositive (+ve)
DulcitolNegative (-ve)
ErythritolVariable
FructosePositive (+ve)
FumaratePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
MucateNegative (-ve)
MyoInositolPositive (+ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinPositive (+ve)
SorbitolPositive (+ve)
SucrosePositive (+ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetate UtilizationVariable
Aesculine HydrolysisPositive (+ve)
Arginine DehydrolaseNegative (-ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LipasePositive (+ve)
Lysine decarboxylasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylasePositive (+ve)
Phenylalanine DeaminaseNegative (-ve)
Tween 80 hydrolysisPositive (+ve)
Tryptophan DeaminaseNegative (-ve)
Tyrosine HydrolysisVariable
Biochemical Tests of Yersinia pestisResults
CapsulePositive (+ve)
CatalasePositive (+ve)
CitrateNegative (-ve)
FlagellaNon-Flagellated
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
Growth in KCNNegative (-ve)
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityNon-Motile
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Facultative anaerobes
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
CellobiosePositive (+ve)
DNaseNegative (-ve)
DulcitolNegative (-ve)
GlucosePositive (+ve)
GlycerolVariable
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseVariable
MucateNegative (-ve)
MyoInositolNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinVariable
SorbitolVariable
SorboseNegative (-ve)
SucroseNegative (-ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Acetate UtilizationNegative (-ve)
Aesculine HydrolysisPositive (+ve)
Arginine DehydrolaseNegative (-ve)
LipaseNegative (-ve)
LysineNegative (-ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Biochemical Tests of Staphylococcus epidermidis Results
CapsuleMostly Capsulated
CatalasePositive (+ve)
CitrateNegative (-ve)
CoagulaseNegative (-ve)
GasPositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingPositive (+ve)
H2SPositive (+ve)
HemolysisNegative (-ve)
MotilityNegative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeCocci
UreasePositive (+ve)
VP (Voges Proskauer)Positive (+ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNaseNegative (-ve)
DulcitolNegative (-ve)
FructosePositive (+ve)
GalactoseVariable
GlucosePositive (+ve)
GlycerolNegative (-ve)
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseVariable
SalicinNegative (-ve)
StarchNegative (-ve)
SucrosePositive (+ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Acetoin ProductionPositive (+ve)
Alkaline PhosphatasePositive (+ve)
Arginine DehydrolaseNegative (-ve)
HyalurodinaseVariable
Ornithine DecarboxylaseVariable
Tryptophan DeaminaseNegative (-ve)
Biochemical Tests of Neisseria gonorrhoeaeResults
CapsuleNegative (-ve)
CatalasePositive (+ve)
GasNegative (-ve)
Gram StainingNegative (-ve)
H2SNegative (-ve)
HemolysisNegative (-ve)
Nitrate ReductionNegative (-ve)
OxidasePositive (+ve)
PigmentNegative (-ve)
ShapeDiplococci
DNaseNegative (-ve)
FructoseNegative (-ve)
GlucosePositive (+ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannoseNegative (-ve)
SucroseNegative (-ve)
TributyrinNegative (-ve)
Biochemical Tests of Enterobacter cloacaeResults
CapsuleNegative (-ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
Gas from glucosePositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
Growth in KCNPositive (+ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityMotile
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Facultative Anaerobes
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
D-ArabitolVariable
CellobiosePositive (+ve)
DNaseNegative (-ve)
DulcitolNegative (-ve)
Meso-ErythritolNegative (-ve)
GlucosePositive (+ve)
GlycerolVariable
LactoseNegative (-ve)
MaltosePositive (+ve)
D-MannitolPositive (+ve)
D-MannosePositive (+ve)
MelibiosePositive (+ve)
MucatePositive (+ve)
MyoInositolNegative (-ve)
RaffinosePositive (+ve)
RhamnosePositive (+ve)
SalicinPositive (+ve)
D-SorbitolPositive (+ve)
SucrosePositive (+ve)
TartrateVariable
TrehalosePositive (+ve)
XylosePositive (+ve)
Acetate UtilizationPositive (+ve)
Aesculine HydrolysisVariable
Arginine decarboxylasePositive (+ve)
Esculin HydrolysisVariable
LipaseNegative (-ve)
Lysine decarboxylasesNegative (-ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylasePositive (+ve)
Pectate degradationNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Tryptophan DeaminaseNegative (-ve)
Biochemical Tests of Clostridium difficileResults
CapsuleCapsulated
CatalaseNegative (-ve)
FlagellaFlagellated
Gelatin HydrolysisPositive (+ve)
Gram StainingPositive (+ve)
H2SPositive (+ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityMotile
Nitrate ReductionNegative (-ve)
OF (Oxidative-Fermentative)Anaerobic
OxidaseNegative (-ve)
ShapeRods
SporePositive (+ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
ArabinoseNegative (-ve)
CellobiosePositive (+ve)
DulcitolVariable
FructosePositive (+ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Casein HydrolysisNegative (-ve)
Esculin HydrolysisPositive (+ve)
LecithinaseNegative (-ve)
LipaseNegative (-ve)
Tryptophan DeaminaseNegative (-ve)
Biochemical Tests of Citrobacter freundiiResults
CapsuleNegative (-ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
Gas from glucosePositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
Growth in KCNPositive (+ve)
H2SPositive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Facultative Anaerobes
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporeNegative (-ve)
UreaseVariable
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
CellobiosePositive (+ve)
DNaseNegative (-ve)
DulcitolNegative (-ve)
GlutamatePositive (+ve)
GlycerolPositive (+ve)
GlycogenPositive (+ve)
InositolNegative (-ve)
LactosePositive (+ve)
LactulosePositive (+ve)
MalonateNegative (-ve)
MaltitoleNegative (-ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibiosePositive (+ve)
MucatePositive (+ve)
MyoInositolPositive (+ve)
PalatinoseNegative (-ve)
RaffinoseVariable
RhamnosePositive (+ve)
SalicinNegative (-ve)
SorbitolPositive (+ve)
SorbosePositive (+ve)
SucrosePositive (+ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Acetate UtilizationPositive (+ve)
Aesculine HydrolysisNegative (-ve)
Arginine DehydrolaseVariable
Esculin HydrolysisNegative (-ve)
LipaseNegative (-ve)
Lysine decarboxylaseNegative (-ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine decarboxylaseNegative (-ve)
Phenylalanine deaminaseNegative (-ve)
Tryptophan deaminaseVariable
Tyrosine hydrolysisPositive (+ve)
Biochemical Tests of Vibrio choleraeResults
CapsuleNon-Capsulated
Citrate+ve
FlagellaFlagellated
Gas-ve
Gelatin Hydrolysis+ve
Gram Staining-ve
H2S-ve
HemolysisBeta Hemolysis
Indole+ve
MotilityMotile
MR (Methyl Red)-ve
Nitrate Reduction+ve
Oxidase+ve
ShapeRods
SporeNon-Sporing
String Test+ve
Urease-ve
VP (Voges Proskauer)Variable
Adonitol-ve
Arabinose-ve
Arabitol-ve
Cellobiose-ve
DNase+ve
Dulcitol-ve
Erythritol-ve
Galactose+ve
Glucose+ve
Glycerol-ve
Inositol-ve
LactoseVariable
Maltose+ve
Mannitol+ve
Mannose+ve
Melibiose-ve
Mucate-ve
MyoInositol-ve
Raffinose-ve
Rhamnose-ve
Salicin-ve
Sorbitol-ve
Sucrose+ve
Tartrate+ve
Trehalose+ve
Xylose-ve
Acetate Utilization+ve
Arbutin-ve
Arginine Dehydrolase-ve
Esculin Hydrolysis-ve
Lipase+ve
Lysine+ve
ONPG (β-galactosidase)+ve
Ornithine Decarboxylase+ve
Phenylalanine Deaminase-ve
Tyrosine Hydrolysis-ve
CharacteristicsEnterococcus faecalis
Gram StainingPositive
Shape (Cocci/Diplococci/Rods)Cocci
Motility (Motile / Non-Motile)Non-Motile
Capsule (Capsulated/Non-Capsulated)
Spore (Sporing/Non-Sporing)Non-Sporing
Flagella (Flagellated/Non-Flagellated)
CatalaseNegative (-ve)
OxidaseNegative (-ve)
MR
VPPositive (+ve)
OF (Oxidative/Fermentative)
IndoleNegative (-ve)
CitrateNegative (-ve)
UreaseNegative (-ve)
Nitrate ReductionPositive (+ve)
H2SNegative (-ve)
Gas
PYRPositive (+ve)
CAMP
Gelatin HydrolysisVariable
Niacin
Coagulase
Hemolysis (Alfa/Beta/Gamma)Variable (Alfa or Beta)
Nagler
String Test
PigmentNegative (-ve)
Bile Solubility
ArabinoseNegative (-ve)
DNase
FructosePositive (D-Fructose)
GlucosePositive (+ve)
HippuratePositive (+ve)
Inositol
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (D-Mannose)
MyoInositol
RaffinoseNegative (D-Raffinose)
RibosePositive (+ve)
SorbitolPositive (+ve)
SucrosePositive (+ve)
Acetate Utilization
Acetoin Production
Acid Phosphatase
Alkaline PhosphataseVariable
Amidase
Arginine dehydrolasePositive (+ve)
Arylsulphatase
Beta Lactamase
Hyalurodinase
Lecithinase
Lipase
Lysine
Neuraminidase
ONPG Test
Ornithine decarboxylase
Phenylalanine deaminase
Perooxidase
Tellurite
Tributyrin
Biochemical Tests of Streptococcus pyogenesResults
CAMPNegative (-ve)
CapsuleCapsulated
CatalaseNegative (-ve)
Gram StainingGram Positive (+ve)
HemolysisBeta Hemolytic
MotilityNon-Motile
OF (Oxidative-Fermentative)Facultative Anaerobes
PYRPositive (+ve)
ShapeCocci
SporeNon-Sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
6.5% NaCl GrowthNegative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseVariable
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolVariable
GlycogenVariable
HippurateNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RiboseNegative (-ve)
SalicinPositive (+ve)
SorbitolNegative (-ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetoin ProductionNegative (-ve)
Alkaline PhosphatasePositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisVariable
HyalurodinasePositive (+ve)
NeuraminidasePositive (+ve)
Biochemical Tests of Shigella flexneriResults
CatalasePositive (+ve)
CitrateNegative (-ve)
GasPositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
Growth in KCNNegative (-ve)
H2SNegative (-ve)
IndoleVariable
MotilityNon-Motile
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporeNon-Sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseVariable
CellobioseNegative (-ve)
DNaseNegative (-ve)
DulcitolNegative (-ve)
GlycerolNegative (-ve)
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltoseVariable
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseVariable
MucateNegative (-ve)
MyoInositolNegative (-ve)
RaffinoseVariable
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolVariable
SucroseNegative (-ve)
TartrateVariable
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetate UtilizationNegative (-ve)
Aesculine HydrolysisNegative (-ve)
Arginine DehydrolaseNegative (-ve)
LipaseNegative (-ve)
LysineNegative (-ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Biochemical Tests of Streptococcus agalactiaeResults
CAMPPositive (+ve)
CapsulePositive (+ve)
CatalaseNegative (-ve)
CoagulaseNegative (-ve)
FlagellaNon-flagellated
Gram StainingPositive (+ve)
HemolysisBeta Hemolysis
MotilityNon-motile
OF (Oxidative-Fermentative)Facultative anaerobes
OxidaseNegative (-ve)
PYRNegative (-ve)
ShapeCocci
SporeNon-sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Variable
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
ArbutinNegative (-ve)
CellobioseVariable
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenNegative (-ve)
HippuratePositive (+ve)
InulinNegative (-ve)
LactoseVariable
MaltosePositive (+ve)
MannitolNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RibosePositive (+ve)
SalicinVariable
SorbitolNegative (-ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Alkaline PhosphatasePositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisNegative (-ve)
CharacteristicsKlebsiella pneumoniae
Capsule+ve
Catalase+ve
Citrate+ve
Flagella-ve
Gas+ve
Gelatin Hydrolysis-ve
Gram Staining-ve
Growth in KCN+ve
H2S-ve
Indole-ve
Motility-ve
MR (Methyl Red)-ve
MUG Test+ve
Nitrate Reduction+ve
OF (Oxidative-Fermentative)Fermentative
Oxidase-ve
Pigment-ve
ShapeRod
Spore-ve
TSIA (Triple Sugar Iron Agar)A/A
Urease+ve
VP (Voges Proskauer)+ve
Adonitol+ve
Arabinose+ve
Arabitol+ve
Cellobiose+ve
DNase-ve
Erythritol-ve
Esculin Hydrolysis+ve
Glucose+ve
Glycerol+ve
Inositol+ve
Lactose+ve
Maltose+ve
Mannitol+ve
Mannose+ve
Melibiose+ve
Mucate+ve
MyoInositol+ve
Raffinose+ve
Rhamnose+ve
Salicin+ve
Sorbitol+ve
Sucrose+ve
Tartrate+ve
Trehalose+ve
Xylose+ve
Acetoin ProductionVariable
Arginine Dehydrolase-ve
Esculin Hydrolysis+ve
Lipase-ve
Lysine+ve
ONPG (β-galactosidase)+ve
Ornithine Decarboxylase-ve
Phenylalanine Deaminase-ve
Tryptophan Deaminase-ve
Tyrosine Hydrolysis-ve
Biochemical Tests of Streptococcus canisResults
CAMPNegative (-ve)
CatalaseNegative (-ve)
Gram StainingPositive (+ve)
OF (Oxidative-Fermentative)Facultative anaerobes
ShapeCocci
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
ArbutinPositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycogenNegative (-ve)
HippurateNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinPositive (+ve)
SorbitolNegative (-ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehaloseVariable
XyloseNegative (-ve)
Alkaline PhosphatasePositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisPositive (+ve)
HyalurodinaseNegative (-ve)
Biochemical Tests of Streptococcus pneumoniaeResults
Bile SolubilityPositive (+ve)
CatalaseNegative (-ve)
Gram StainingPositive (+ve)
HemolysisAlfa Hemolysis
MotilityNon-motile
OF (Oxidative-Fermentative)Facultative anaerobes
OxidaseNegative (-ve)
ShapeDiplococci
SporeNon-sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
ArabinosePositive (+ve)
ArbutinNegative (-ve)
DulcitolNegative (-ve)
ErythritolPositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenPositive (+ve)
HippurateNegative (-ve)
InulinPositive (+ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MelibioseVariable
RaffinosePositive (+ve)
RiboseNegative (-ve)
SalicinVariable
SorbitolNegative (-ve)
StarchVariable
SucrosePositive (+ve)
TrehaloseVariable
XylosePositive (+ve)
Alkaline PhosphataseNegative (-ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisVariable
HyalurodinasePositive (+ve)
NeuraminidasePositive (+ve)
Biochemical Tests of Bacillus anthracisResults
CapsuleCapsulated
CatalasePositive (+ve)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingPositive (+ve)
HemolysisNegative (-ve)
IndolePositive (+ve)
MotilityNegative (-ve)
Nitrate ReductionPositive (+ve)
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporePositive (+ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructoseVariable
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
GlycogenPositive (+ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Arginine DehydrolasePositive (+ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LecithinaseVariable
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Tyrosine HydrolysisNegative (-ve)
Biochemical Tests of Clostridium tetani Results
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaPeritrichous
GasPositive (+ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-stain-positive, but become Gram-stain-negative after approximately 24 h incubation.
Growth in 20% BileNegative (-ve)
Growth in 6.5% NaClNegative (-ve)
H2SPositive (+ve)
HemolysisPositive (+ve)
IndoleVariable
MotilityPositive (+ve)
Nitrate ReductionNegative (-ve)
ShapeLarge rods (0.5-1.7 x 2.1-18.1 µm), which may appear single, in pairs or in short chains.
SporePositive (+ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNasePositive (+ve)
FructoseNegative (-ve)
GalactoseNegative (-ve)
GlucoseNegative (-ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Esculin HydrolysisNegative (-ve)
LecithinaseNegative (-ve)
LipaseNegative (-ve)
NeuraminidaseNegative (-ve)
Superoxide dismutase Negative (-ve)
Biochemical Tests of Aeromonas hydrophila Results
Capsule-ve
Catalase+ve
Citrate+ve
Flagella+ve
Gas+ve
Gelatin Hydrolysis+ve
Gram StainingGram-negative
H2S+ve
Hemolysis+ve
Indole+ve
Maximum Growth Temperature41°C
Motility+ve
Nitrate Reduction+ve
OF (Oxidative-Fermentative)Fermentative
Oxidase+ve
Oxygen RequirementFacultative Anaerobe
ShapeRod
Spore-ve
String Test-ve
TSIA (Triple Sugar Iron Agar)Alkali/Acid
Urease-ve
VP (Voges Proskauer)+ve
ArabinoseVariable
Arabitol-ve
DNase+ve
Dulcitol-ve
Erythritol-ve
Glucose+ve
GluconateVariable
LactoseVariable
Mannitol+ve
Mucate-ve
Rhamnose-ve
Sorbitol-ve
Sucrose+ve
Trehalose+ve
Xylose-ve
Arbutin+ve
Arginine Dehydrolase+ve
Elastase+ve
Esculin Hydrolysis+ve
Lysine Decarboxylase+ve
Ornithine Decarboxylase-ve
Pectinase+ve
Phenylalanine Deaminase-ve
Pyrazinamidase+ve
Biochemical Tests of Acinetobacter baumannii Results
Bile SolubilityNegative (-ve)
CapsuleCapsulated
CatalasePositive (+ve)
CitratePositive (+ve)
CoagulaseNegative (-ve)
FlagellaNon-Flagellated
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityNon-Motile
MR (Methyl Red)Negative (-ve)
Nitrate ReductionNegative (-ve)
OF (Oxidative-Fermentative)Non- Fermentative (Oxidative)
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeCoccobacillus
SporeNon-Sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
DNaseNegative (-ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
LactateVariable (+/-)
LactoseMostly Negative (-ve), Some Positive (+ve)
MalonatePositive (+ve)
MaltoseVariable (+/-)
MannitolNegative (-ve)
MannosePositive (+ve)
RhamnosePositive (+ve)
SucroseNegative (-ve)
XylosePositive (+ve)
Arginine DehydrolasePositive (+ve)
AzelatePositive (+ve)
Beta LactamasePositive (+ve)
Esculin HydrolysisNegative (-ve)
HistamineNegative (-ve)
HistidinePositive (+ve)
LeucinePositive (+ve)
LysineVariable (+/-)
MalatePositive (+ve)
Phenylalanine DeaminasePositive (+ve)
Tyrosine HydrolysisPositive (+ve)
Biochemical Tests of Fusobacterium necrophorum Results
Bile GrowthVariable
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasPositive (+ve)
Gelatin HydrolysisVariable
Gram StainingGram-negative bacteria
H2SPositive (+ve)
HemolysisPositive (+ve) (alpha- and beta-hemolysis may be observed)
IndolePositive (+ve)
Methylene Blue ReductionPositive (+ve)
MotilityNegative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
ShapePleomorphic rod ranging from small, almost coccoid bodies to filaments, with parallel sides and blunt or tapering ends.
SporeNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNasePositive (+ve)
DulcitolNegative (-ve)
FructoseVariable
GlucoseVariable
GlycerolNegative (-ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SorbitolNegative (-ve)
SucroseNegative (-ve)
N-Acetyl-glucosaminidaseNegative (-ve)
Acid PhosphatasePositive (+ve)
Alkaline PhosphatasePositive (+ve)
Beta-LactamasePositive (+ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisNegative (-ve)
β -GlucosidaseNegative (-ve)
LecithinaseNegative (-ve)
LipasePositive (+ve)
Lysine decarboxylaseNegative (-ve)
PhosphataseNegative (-ve)
TryptophanasePositive (+ve)
Biochemical Tests for Haemophilus aegyptius Results
CapsuleNegative (-ve)
CatalasePositive (+ve)
GasNegative (-ve)
Gram Staining.Gram-negative
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
Nitrate ReductionPositive (+ve)
Nitrite ReductionNegative (-ve)
OxidasePositive (+ve)
ShapeLong slender rods
UreasePositive (+ve)
V-factor requirementPositive (+ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructoseNegative (-ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
SorboseNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XylitolNegative (-ve)
XylosePositive (+ve)
Alkaline PhosphatasePositive (+ve)
Arginine DihydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
FucosidaseNegative (-ve)
IgA1 proteasePositive (+ve)
Lysine decarboxylaseNegative (-ve)
NeuraminidasePositive (+ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Biochemical Tests for Burkholderia cepacia Results
BacteriocinPositive (+ve)
CapsulePositive (+ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
Gelatin HydrolysisVariable
Gram StainingGram-stain-negative
HemolysisNegative (-ve)
MotilityPositive(+ve)
Nitrate ReductionNegative (-ve)
OxidaseVariable
PigmentPositive (+ve)
Shape                  Straight or curved rods
SporeNegative (-ve)
UreasePositive (+ve)
AdonitolPositive (+ve)
AmygdalinPositive (+ve)
ArabinosePositive (+ve)
ArabitolPositive (+ve)
ArbutinPositive (+ve)
CellobioseVariable
DulcitolPositive (+ve)
ErythritolNegative (-ve)
FucosePositive (+ve)
GlucosaminePositive (+ve)
2-KetogluconatePositive (+ve)
5-KetogluconatePositive (+ve)
LactoseNegative (-ve)
MalonatePositive (+ve)
MaltoseVariable
MelibioseVariable
MucatePositive (+ve)
N-AcetylglucosaminePositive (+ve)
RaffinoseVariable
RhamnoseVariable
RibosePositive (+ve)
SalicinPositive (+ve)
StarchNegative (-ve)
SucroseVariable
TagatosePositive (+ve)
TartratePositive (+ve)
TrehaloseNegative (-ve)
XyloseVariable
Arginine DihydrolaseNegative (-ve)
Beta LactamasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylaseVariable
Phospholipase CPositive (+ve)
ProteasePositive (+ve)
Biochemical Tests for Klebsiella granulomatis Results
CapsulePositive (+ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaNegative (-ve)
Gram StainingGram-negative
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityNon-motile
MR (Methyl Red)Negative (-ve)
Nitrate ReductionNegative (-ve)
OxidasePositive (+ve)
ShapePleomorphic rods (coccobacillus) with rounded ends.

Occur singly or in clusters

UreasePositive (+ve)
VP (Voges Proskauer)Positive (+ve)
ArabinoseNegative (-ve)
DulcitolNegative (-ve)
GlucosePositive (+ve)
LactosePositive (+ve)
MalonatePositive (+ve)
RaffinoseNegative (-ve)
RhamnosePositive (+ve)
XylosePositive (+ve)
Lysine decarboxylasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Biochemical Tests for Haemophilus parainfluenzae Results
CapsuleVariable
CatalaseVariable
GasVariable
Gram StainingGram-negative (-ve)
H2SPositive (+ve)
HemolysisNegative (-ve)
IndoleVariable
MotilityNegative (-ve)
Nitrate ReductionPositive (+ve)
Nitrite ReductionPositive (+ve)
OxidasePositive (+ve)
ShapeSmall, pleomorphic rods, usually with long, filamentous forms.
UreaseVariable
V-factor requirementPositive (+ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolVariable
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
RaffinoseVariable
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
SorboseNegative (-ve)
SucrosePositive (+ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Alkaline PhosphatasePositive (+ve)
Arginine DihydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
FucosidaseNegative (-ve)
IgA1 proteaseNegative (-ve)
Lysine decarboxylaseVariable
ONPG (β-galactosidase)Variable
Ornithine DecarboxylaseVariable
Biochemical Tests for Francisella tularensis subsp. tularensis Results
CapsulePositive (+ve)
CatalasePositive (+ve)
FlagellaNegative (-ve)
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingFaintly stained Gram-negative
H2S (in cysteine-supplemented medium)Positive (+ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
Nitrate ReductionNegative (-ve)
NH3 produced in liquid mediaNegative (-ve)
OxidaseNegative
ShapeShort, rod-shaped or coccoid
SporeNegative (-ve)
Sodium ricinoleate solubilityPositive (+ve)
UreaseNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
SucroseNegative (-ve)
Beta-LactamasePositive (+ve)
Citrulline ureidase (CTU)Positive (+ve)
Biochemical Tests for Kingella kingae Results
10% BileNegative (-ve)
CapsulePositive (+ve)
CatalaseNegative (-ve)
CitrateNegative (-ve)
CoagulaseNegative (-ve)
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram Staining Gram-negative
H2SVariable
HemolysisPositive (+ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
6% NaClNegative (-ve)
Nitrate ReductionNegative (-ve)
Nitrite ReductionNegative (-ve)
OF (Oxidative-Fermentative)Fermentative
OxidasePositive (+ve)
ShapeCoccobacilli (0.6 to 1 μm by 1 to 3 μm)
SporeNegative (-ve)
UreaseNegative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
DNaseNegative (-ve)
DulcitolNegative (-ve)
FructoseNegative (-ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
InositolNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Acid PhosphatasePositive (+ve)
Alkaline PhosphatasePositive (+ve)
Arginine DihydrolaseNegative (-ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisNegative (-ve)
LecithinaseNegative (-ve)
LipaseNegative (-ve)
Lysine decarboxylaseNegative (-ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Biochemical Tests for Bordetella pertussis Results
CapsulePositive (+ve)
CatalasePositive (+ve)
CitrateNegative (-ve)
FlagellaNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-negative (-ve)
Growth in nutrient brothNegative (-ve)
HemolysisPositive (+ve)
MotilityNegative (-ve)
Nitrate ReductionNegative (-ve)
OxidasePositive(+ve)
PigmentNegative (-ve)
ShapeMinute coccobacilli
SporeNegative (-ve)
UreaseNegative (-ve)
AdipateNegative (-ve)
CaprateNegative (-ve)
Chymotrypsin activityPositive (+ve)
GluconateNegative (-ve)
GlucoseNegative (-ve)
LactoseNegative (-ve)
MalateNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
PhenylacetateNegative (-ve)
SucroseNegative (-ve)
XyloseNegative (-ve)
Alkaline PhosphataseNegative (-ve)
Esculin HydrolysisNegative (-ve)
Ester C8 lipase activityPositive (+ve)
Lipase C14 activityNegative (-ve)
Lysine decarboxylaseNegative (-ve)
Naphthol-AS-B1 phosphohydrolase activityPositive (+ve)
TrypsinNegative (-ve)
Biochemical Tests for Lactobacillus spp. Results
CapsuleNegative (-ve)
CatalaseNegative (-ve)
CitrateNegative (-ve)
FlagellaIf present, peritrichous
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram positive
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityMostly Negative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
PigmentRare; if present, yellow or orange to rust or brick red.
Shapelong and slender, sometimes bent rods to short, often coryneform coccobacilli
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AmygdalinPositive (+ve)
ArabinosePositive (+ve)
CellobiosePositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
MelibioseVariable
RaffinoseVariable
RhamnosePositive (+ve)
RiboseNegative (-ve)
SalicinPositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehaloseVariable
XyloseVariable
Casein HydrolysisNegative (-ve)
Esculin HydrolysisPositive (+ve)
Biochemical Tests for Haemophilus influenzae Results
CapsuleStrain-dependent
CatalasePositive (+ve)
FlagellaNegative (-ve)
GasNegative (-ve)
Gram StainingGram-negative
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleVariable
MotilityNegative (-ve)
Nitrate ReductionPositive (+ve)
Nitrite ReductionNegative (-ve)
OxidasePositive (+ve)
PigmentAbsent or yellowish
ShapeCoccobacilli or small regular rods
UreaseVariable
V-factor requirementPositive (+ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructoseNegative (-ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
SorboseNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XylosePositive (+ve)
Alkaline PhosphatasePositive (+ve)
Arginine DihydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
FucosidaseNegative (-ve)
IgA1 proteasePositive (+ve)
Lysine decarboxylaseVariable
NeuraminidasePositive (+ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylaseVariable
Biochemical Tests for Klebsiella oxytoca Results
CapsulePositive (+ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaNegative (-ve)
GasVariable
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-negative
Growth in KCNPositive (+ve)
H2SNegative (-ve)
IndolePositive (+ve)
MotilityNegative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeCylindrical rod shaped which appear singly, in pairs or in short chains
SporeNegative (-ve)
TSIA (Triple Sugar Iron Agar)Acid/ Acid; Gas positive; H2S negative
UreasePositive (+ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolPositive (+ve)
ArabinosePositive (+ve)
ArabitolVariable
CellobiosePositive (+ve)
DNaseNegative (-ve)
DulcitolVariable
ErythritolNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
InositolPositive (+ve)
LactosePositive (+ve)
MalonateVariable
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibiosePositive (+ve)
MelezitosePositive (+ve)
MucatePositive (+ve)
RaffinosePositive (+ve)
RhamnosePositive (+ve)
SalicinPositive (+ve)
SorbitolPositive (+ve)
SucrosePositive (+ve)
TartratePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Aesculine HydrolysisPositive (+ve)
Arginine DehydrolaseNegative (-ve)
Esculin HydrolysisPositive (+ve)
Gluconate dehydrogenaseNegative (-ve)
LipaseNegative (-ve)
Lysine DecarboxylasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylaseNegative (-ve)
Pectate DegradationPositive (+ve)
Phenylalanine DeaminasePositive (+ve)
Biochemical Tests for Gardnerella vaginalis Results
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram negative (-ve) or Gram variable
H2SNegative (-ve)
HemolysisPositive (+ve) (Human or Rabbit Blood)
IndoleNegative (-ve)
MotilityNegative (-ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
ShapePleomorphic Rods
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseVariable
ArabitolNegative (-ve)
ArbutinNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
FructoseVariable
GalactoseVariable
GlucosePositive (+ve)
GlycerolNegative (-ve)
HippuratePositive (+ve)
InositolNegative (-ve)
InulinVariable
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseVariable
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinNegative (-ve)
SorbitolPositive (+ve)
StarchPositive (+ve)
SucroseNegative (-ve)
TagatoseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Arginine DecarboxylaseNegative (-ve)
Casein HydrolysisVariable
Esculin HydrolysisNegative (-ve)
EsteraseNegative (-ve)
F6PPKPositive (+ve)
a-D-glucosidasePositive (+ve)
LipaseVariable
Lysine dihydrolaseNegative (-ve)
N-acetyl-a-d-glucosaminidasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminasePositive (+ve)
PhospholipasePositive (+ve)
TributyrinNegative (-ve)
Biochemical Tests for Burkholderia pseudomallei Results
CapsulePositive (+ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-negative (-ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
Nitrate ReductionPositive (+ve)
OxidasePositive (+ve)
PigmentNegative (-ve)
ShapeRod-shaped; “safety pin” appearance (bipolar staining)
SporeNegative (-ve)
TSIA (Triple Sugar Iron Agar)K/NC/H2S -ve
UreaseNegative (-ve)
ArabinosePositive (+ve)
ArabitolPositive (+ve)
CellobiosePositive (+ve)
DulcitolPositive (+ve)
ErythritolPositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
HippuratePositive (+ve)
InositolPositive (+ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolVariable
MannosePositive (+ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetate UtilizationPositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisVariable
LecithinasePositive (+ve)
LipasePositive (+ve)
Ornithine DecarboxylasePositive (+ve)
Superoxide DismutasePositive (+ve)
Biochemical Tests for Edwardsiella tarda Results
CatalasePositive (+ve)
CitrateNegative (-ve)
FlagellaPeritrichous
GasPositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-negative
Growth in KCNNegative (-ve)
H2SPositive (+ve)
IndolePositive (+ve)
MotilityPositive (+ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OxidaseNegative (-ve)
ShapeStraight Rods
TSIA (Triple Sugar Iron Agar)K/A
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseVariable
ArabitolNegative (-ve)
CellobioseNegative (-ve)
DNaseNegative (-ve)
DulcitolNegative (-ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
InositolNegative (-ve)
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltosePositive (+ve)
MannitolVariable
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseVariable
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Aesculine HydrolysisNegative (-ve)
AmylaseNegative (-ve)
Arginine DehydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
Beta-GlucuronidaseNegative (-ve)
LipaseNegative (-ve)
Lysine DecarboxylasePositive (+ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylasePositive (+ve)
Phenylalanine DeaminaseNegative (-ve)
L-Pyrrolidonyl aminopeptidaseNegative (-ve)
Biochemical Tests for Enterobacter aerogenes Results
CapsulePositive (+ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
GasPositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-negative
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
ShapeRod-shaped
SporeNegative (-ve)
TSIA (Triple Sugar Iron Agar)Acid/ Acid Gas
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
CellobiosePositive (+ve)
DNaseNegative (-ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
LactosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
RhamnosePositive (+ve)
StarchNegative (-ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Arginine DehydrolaseNegative (-ve)
Esculin HydrolysisPositive (+ve)
Gamma-Glutamyl transferasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylasePositive (+ve)
Phenylalanine DeaminaseNegative (-ve)
Tetrathionate reductaseNegative (-ve)
Biochemical Tests for Aeromonas caviae Results
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaVariable (+/-)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram negative
Growth in KCNPositive (+ve)
Growth in 0% NaClPositive (+ve)
Growth in 3% NaClPositive (+ve)
H2SNegative (-ve)
HemolysisVariable (+/-)
IndolePositive (+ve)
MotilityVariable (+/-)
Nitrate ReductionPositive (+ve)
OxidasePositive (+ve)
ShapeRod
SporeNegative (-ve)
String TestNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
CellobioseVariable (+/-)
DNasePositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
Glucose (gas)Negative (-ve)
GlycerolVariable (+/-)
InositolNegative (-ve)
LactoseVariable (+/-)
MalonateNegative (-ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannoseVariable (+/-)
MelibioseNegative (-ve)
MucateNegative (-ve)
Phenylpyruvic acid productionPositive (+ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinPositive (+ve)
SorbitolNegative (-ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetate UtilizationPositive (+ve)
ArbutinPositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisPositive (+ve)
ElastaseNegative (-ve)
LipaseVariable (+/-)
LysineNegative (-ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylaseNegative (-ve)
Biochemical Tests for Alcaligenes  faecalis subsp. faecalis Results
Bromocresol purple milkAlkaline reaction
CapsuleNegative (-ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-negative (-ve)
Growth in 7% NaClPositive (+ve)
H2SNegative (-ve)
HemolysisPositive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
Nitrate ReductionNegative (-ve)
Nitrite ReductionPositive (+ve)
OxidasePositive (+ve)
PigmentNegative (-ve)
ShapeRods or coccobacilli
SporeNegative (-ve)
Tween 80Negative (-ve)
UreaseNegative (-ve)
AcetatePositive (+ve)
AdonitolNegative (-ve)
AdipateNegative (-ve)
AlaninePositive (+ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CelluloseNegative (-ve)
DNaseNegative (-ve)
FructoseNegative (-ve)
GalactoseNegative (-ve)
GlucoseNegative (-ve)
GluconateNegative (-ve)
GlutamatePositive (+ve)
GlycerolNegative (-ve)
GlycogenNegative (-ve)
LactatePositive (+ve)
MalatePositive (+ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
PropionatePositive (+ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SuccinatePositive (+ve)
SucroseNegative (-ve)
TartrateNegative (-ve)
XyloseNegative (-ve)
Esculin HydrolysisNegative (-ve)
Lysine DecarboxylaseNegative (-ve)
Ornithine DecarboxylasePositive (+ve)
Biochemical Tests for Chlamydia trachomatis Results
Organism TypeObligate intracellular organism that parasitize and multiply in the cytoplasm of eukaryotic cells within membrane bound vacuoles, termed inclusions, by a unique developmental cycle.
ATP/ADP Transport SystemNegative (-ve)
Body TypesElementary and Reticulate
FlagellaAbsent
Gram StainingGram-negative
Genus Specific AntigenPositive (+ve)
MotilityNegative (-ve)
Projections on SurfacePositive (+ve)
ShapeCoccoid; 0.2-1.5 Um diameter
ArginineNegative (-ve)
CysteineNegative (-ve)
GlutamineVariable
HistidinePositive (+ve)
IsoleucineNegative (-ve)
LeucineVariable
LysineNegative (-ve)
MethionineVariable
PhenylalaninePositive (+ve)
ThreonineNegative (-ve)
TryptophanVariable
TyrosineNegative (-ve)
ValinePositive (+ve)
Glucose-6-P (ATP-dependent liberation of CO2 )Positive (+ve)
Glutamate (ATP-dependent liberation of CO2 )Positive (+ve)
Pyruvate (ATP-dependent liberation of CO2 )Positive (+ve)
Biochemical Tests for Enterococcus faecium Results
40% Bile SaltsPositive (+ve)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
CitrateNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-positive
H2SNegative (-ve)
HemolysisVariable
0.1% Methylene blue milkPositive (+ve)
MotilityNegative (-ve)
6.5% NaClPositive (+ve)
PigmentNegative (-ve)
ShapeCocci
SporeNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
ArbutinPositive (+ve)
CellobiosePositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
FucoseNegative (-ve)
GalactosePositive (+ve)
Beta- GentiobiosePositive (+ve)
GlucosePositive (+ve)
GlycerolVariable
GlycogenNegative (-ve)
HippurateVariable
InositolNegative (-ve)
LactosePositive (+ve)
MalateNegative (-ve)
MaltosePositive (+ve)
MannitolVariable
MannosePositive (+ve)
MelibioseVariable
RaffinoseVariable
RhamnoseVariable
SorbitolVariable
StarchNegative (-ve)
SucroseVariable
TrehaloseVariable
XyloseVariable
Alkaline PhosphataseNegative (-ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisPositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Pyrrolidonyl aminopeptidasePositive (+ve)
TelluriteNegative (-ve)
Biochemical Tests for Clostridium botulinum Results
Ammonia ProductionPositive (+ve)
20% BileNegative (-ve)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaPeritrichous
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-positive
H2SPositive (+ve)
HemolysisPositive (+ve). β-hemolytic
IndoleNegative (-ve)
Milk DigestionMostly Positive (+ve)
Meat DigestionMostly Positive (+ve)
MotilityMostly Positive (+ve)
6.5% NaClNegative (-ve)
OxidaseNegative (-ve)
ShapeStraight to slightly curved rods, 0.6–1.4 × 3.0–20.2 μm.
SporePositive (+ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
FructoseNegative (-ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycogenNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
ArgininePositive (+ve)
GlycinePositive (+ve)
PhenylalaninePositive (+ve)
ProlinePositive (+ve)
SerinePositive (+ve)
TyrosinePositive (+ve)
TryptophanPositive (+ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LecithinaseNegative (-ve)
LipasePositive (+ve)
Superoxide dismutasePositive (+ve)
Tryptophan DeaminaseNegative (-ve)
Biochemical Tests for Enterococcus faecalis Results
Bile ResistanceResistance to 40% (v/v) bile
CapsuleNegative (-ve)
CatalaseNegative (-ve)
CitrateNegative (-ve)
FlagellaNegative (-ve)
Gelatin HydrolysisVariable
Gram StainingGram-positive
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
6.5% NaClPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentNegative (-ve)
PYRPositive (+ve)
ShapeCocci
Sodium azidePositive (+ve)
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolPositive (+ve)
ArabinoseNegative (-ve)
ArabitolPositive (+ve)
ArbutinPositive (+ve)
CellobiosePositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructoseNegative (-ve)
FucoseNegative (-ve)
GalactosePositive (+ve)
GentiobiosePositive (+ve)
GlucosePositive (+ve)
GluconateVariable
GlycerolPositive (+ve)
GlycogenNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
PyruvatePositive (+ve)
RaffinoseNegative (-ve)
RhamnoseVariable
RibosePositive (+ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acetoin ProductionPositive (+ve)
Alkaline PhosphataseNegative (-ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisPositive (+ve)
Beta GlucuronidaseNegative (-ve)
HippuratePositive (+ve)
ONPG (β-galactosidase)Negative (-ve)
Pyrrolidonyl arylamidasePositive (+ve)
Biochemical Tests for Campylobacter fetus Results
CapsuleCapsule like surface (S-layer) proteins
CatalasePositive (+ve)
Gram StainingGram-negative
KMnO4Positive (+ve)
H2SNegative (-ve)
HemolysisNegative (-ve)
MotilityPositive (+ve)
2% NaClPositive (+ve)
Nitrate ReductionPositive (+ve)
2% Ox-bilePositive (+ve)
OxidasePositive (+ve)
0.02% SafraninPositive (+ve)
ShapeSlender curved rods; comma-, S-, and gull-shaped; ends of the cells

are pointed.

SporeNegative (-ve)
UreaseNegative (-ve)
ArabinoseNegative (-ve)
GalactoseNegative (-ve)
Glucose (8%)Positive (+ve)
1% GlycinePositive (+ve)
HippurateNegative (-ve)
Indoxyl acetate hydrolysisNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannoseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SucroseNegative (-ve)
HippucaseNegative (-ve)
Biochemical Tests for Clostridium perfringens Results
20% BilePositive (+ve)
CapsulePositive (+ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasPositive (+ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-positive
HemolysisPositive (+ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
Nitrate ReductionVariable
OxidaseNegative (-ve)
ShapeRod shaped; straight rods with blunt ends
SporePositive (+ve)
2 % NaClPositive (+ve)
6.5% NaClNegative (-ve)
ArabinoseNegative (-ve)
CellobioseVariable
DNasePositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolWeakly Positive (+ve)
GlycogenVariable
InositolVariable
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
RaffinoseVariable
RiboseVariable
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchVariable
SucrosePositive (+ve)
TrehaloseVariable
XyloseNegative (-ve)
AmylasePositive (+ve)
ElastasePositive (+ve)
Esculin HydrolysisVariable
HyalurodinasePositive (+ve)
LecithinasePositive (+ve)
LipaseNegative (-ve)
NeuraminidasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
RibonucleasePositive (+ve)
Superoxide dismutasePositive (+ve)
Biochemical Tests for B. melitensis Results
CapsuleNegative (-ve)
CatalasePositive(+ve)
FlagellaNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram Negative (-ve)
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
Litmus MilkNegative (-ve)
MotilityNegative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive(+ve)
OxidasePositive(+ve)
ShapeCoccobacilli, or short rods
SporeNegative (-ve)
UreasePositive(+ve)
VP (Voges Proskauer)Negative (-ve)
L-ArabinoseNegative (-ve)
ErythritolPositive(+ve)
D-GalactoseNegative (-ve)
L- GlucosePositive(+ve)
Oxidation of L- AlaninePositive (+ve)
Oxidation of L-AsparaginePositive (+ve)
Oxidation of L-Glutamic acidPositive (+ve)
D-RiboseNegative (-ve)
D-XyloseNegative (-ve)
Arginine DehydrolaseNegative (-ve)
CitrullineNegative (-ve)
Glycylglycine-b-naphthylamidasePositive (+ve)
Glycine-b-naphthylamidasePositive (+ve)
3-Indoxyl phosphataseNegative (-ve)
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
p-Nitrophenyl-beta-d-galactopyranosidaseNegative (-ve)
p-Nitrophenyl-alpha-d-galactopyranosidaseNegative (-ve)
p-Nitrophenyl-N-acetyl-b-d-glucosaminidasePositive (+ve)
p-Nitrophenyl-alpha-d-glucopyranosidasePositive (+ve)
p-Nitrophenyl phosphatasePositive (+ve)
Biochemical tests for Bifidobacterium bifidum Results
2% Bile SaltPositive (+ve)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
FlagellaNegative (-ve)
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-Positive (+ve)
IndoleNegative (-ve)
MotilityNegative (-ve)
Nitrate ReductionNegative (-ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapePleomorphic rods
SporeNegative (-ve)
AmyloseVariable
CellobioseNegative (-ve)
Fructose-6-PhosphatePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
InulinNegative (-ve)
LactosePositive (+ve)
MaltasePositive (+ve)
MaltoseVariable
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseVariable
PectinNegative (-ve)
RaffinoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseVariable
TrehaloseNegative (-ve)
XylanNegative (-ve)
XyloseNegative (-ve)
ArabinosidasesPositive (+ve)
Fructose 6-phosphoketolasePositive (+ve)
GlucosidasesPositive (+ve)
Glutamate dehydrogenasePositive (+ve)
Glutamine synthetasePositive (+ve)
HexosaminidasesPositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Biochemical Tests For Bacteroides fragilis Results
CapsulePositive (+ve)
CatalasePositive (+ve)
FlagellaNegative (-ve)
Gelatin HydrolysisWeakly Positive (+ve)
Gram StainingGram-negative (-ve)
Growth in Bile (20%)Positive (+ve)
HemolysisNegative (-ve)
IndoleVariable
MotilityNegative (-ve)
Nitrate ReductionNegative (-ve)
OxidaseVariable
PigmentNegative (-ve)
ShapeRod-shaped cells with rounded ends.
SporeNegative (-ve)
Thioglycolate with bilePositive (+ve)
UreaseNegative (-ve)
ArabinoseNegative (-ve)
CellobioseVariable
GlucosePositive(+ve)
GlycogenPositive(+ve)
InositolNegative (-ve)
LactosePositive(+ve)
MaltosePositive(+ve)
MannitolNegative (-ve)
MannosePositive(+ve)
MelibioseWeakly Positive (+ve)
RaffinosePositive(+ve)
RhamnoseNegative (-ve)
RiboseNegative (-ve)
SalicinNegative (-ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehaloseNegative (-ve)
XylanNegative (-ve)
XylosePositive (+ve)
Alanine arylamidasePositive (+ve)
Esculin HydrolysisPositive (+ve)
Glutamic acid decarboxylasePositive (+ve)
Glycine arylamidaseNegative (-ve)
Leucine arylamidasePositive (+ve)
Leucyl glycine arylamidasePositive (+ve)
N-Acety1- b-glucosaminidasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Biochemical Tests For Actinomyces israelii Results
CAMP-ve
Capsule-ve
Catalase-ve
Gelatin Hydrolysis-ve
Gram StainingGram +ve
Hemolysis-ve
Indole-ve
Motility-ve
MR (Methyl Red)Variable
Nitrate ReductionVariable
OF (Oxidative-Fermentative)Fermentative
Pigment-ve
ShapeBranching filaments, branched rods

and diphtheroidal forms

Urease-ve
VP (Voges Proskauer)-ve
Cellobiose+ve
DNase-ve
Dulcitol-ve
Fructose+ve
Galactose+ve
Glucose+ve
GlycogenVariable
Hippurate-ve
InositolVariable
Lactose+ve
Maltose+ve
Mannitol+ve
Mannose+ve
MelibioseVariable
MyoInositolVariable
Raffinose+ve
Rhamnose-ve
RiboseVariable
SalicinVariable
SorbitolVariable
Starch-ve
Sucrose+ve
TrehaloseVariable
Xylose+ve
Acetoin Production-ve
Casein Hydrolysis-ve
Esculin Hydrolysis+ve
α -Fucosidase-ve
α-Glucosidase+ve
Lipase-ve
β-NAG-ve
ONPG (β-galactosidase)+ve
Biochemical Test Of Listeria monocytogenes Results
CAMPPositive (+ve)
CatalasePositive (+ve)
FlagellaFlagellated
GasNegative (-ve)
Gram StainingPositive (+ve)
H2SNegative (-ve)
HemolysisBeta Hemolysis
IndoleNegative (-ve)
MotilityMotile
MR (Methyl Red)Positive (+ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
ShapeRods
SporeNon-Sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
ArabinoseNegative (-ve)
DextrinVariable
GalactoseVariable
GluconateNegative (-ve)
D-GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenNegative (-ve)
HippuratePositive (+ve)
LactosePositive (+ve)
D-MannitolNegative (-ve)
MelezitosePositive (+ve)
MelibioseNegative (-ve)
RhamnosePositive (+ve)
RiboseNegative (-ve)
SorbitolVariable
StarchNegative (-ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Acid PhosphatasePositive (+ve)
Arginine DehydrolasePositive (+ve)
CelluloseNegative (-ve)
Esculin HydrolysisPositive (+ve)
LecithinasePositive (+ve)
TryptophanaseNegative (-ve)
Biochemical Tests For Helicobacter pylori Results
CapsuleNon-Capsulated
CatalasePositive (+ve)
FlagellaFlagellated
Glycine (1%) ToleranceNegative (-ve)
Gram StainingNegative (-ve)
Growth at 25, 30 and 45°CNegative (-ve)
Growth at 35 and 40°CPositive (+ve)
Growth at 0.5 and 0.75 % NaClPositive (+ve)
Growth at 1.00 and 1.25 % NaClPositive (+ve)
Growth at 1.5 % NaClNegative (-ve)
H2SPositive (+ve)
MotilityMotile
Nitrate ReductionNegative (-ve)
OxidasePositive (+ve)
ShapeRods
SporeNon-Sporing
UreasePositive (+ve)
GlucosePositive (+ve)
Alkaline PhosphatasePositive (+ve)
HippurateNegative (-ve)
Indoxyl AcetateNegative (-ve)
Biochemical Tests of Campylobacter jejuni Results
CapsuleCapsulated
CatalasePositive (+ve)
FlagellaFlagellated
Gram StainingNegative (-ve)
Growth at 25°CNegative (-ve)
Growth at 37°CPositive (+ve)
Growth at 42°CPositive (+ve)
Growth on MacConkey AgarPositive (+ve)
H2SNegative (-ve)
HemolysisNon-Hemolytic
KOHPositive (+ve)
MotilityMotile
Nitrate ReductionPositive (+ve)
Nitrite ReductionNegative (-ve)
OF (Oxidative-Fermentative)Non-Fermentative
OxidasePositive (+ve)
Resistance to cephalothinPositive (+ve)
Resistance to nalidixic acidNegative (-ve)
ShapeComma/S-Shaped/Coccoid/Rods
SporeNon-Sporing
UreaseNegative (-ve)
DNaseNegative (-ve)
GlucoseNegative (-ve)
Alkaline PhosphataseVariable
ArylsulphataseNegative (-ve)
HippuratePositive (+ve)
Indoxyl AcetatePositive (+ve)
Tween 40Positive (+ve)
Tween 60Positive (+ve)
Tween 80Negative (-ve)
Biochemical Tests of Mycobacterium tuberculosis Results
5 % NaCl ToleranceNegative (-ve)
68°C Catalase TestNegative (-ve)
Acid Fast StainPositive (+ve)
Acid PhosphataseNegative (-ve)
Amidase TestPositive (+ve)
Arylsulphatase TestNegative (-ve)
Growth at 22°CNegative (-ve)
Growth at 25°CNegative (-ve)
Growth at 42°CNegative (-ve)
Growth at 25°CNegative (-ve)
Growth on P-Nitrobenzoic AcidNegative (-ve)
Growth on TCH (10 mg/ml)Positive (+ve)
Iron UptakeNegative (-ve)
Neutral Red TestPositive (+ve)
Niacin TestPositive (+ve)
Nitrate Reduction TestPositive (+ve)
PigmentationNegative (-ve)
Peroxidase TestPositive (+ve)
Pyrazinamidase TestPositive (+ve)
Pyruvate stimulation growthNegative (-ve)
Semi Quantitative Catalase TestNegative (-ve)
Tellurite Reduction TestNegative (-ve)
Tween 80 HydrolysisNegative (-ve)
Urease TestPositive (+ve)
Biochemical Tests of Corynebacterium diphtheriae Results
CAMPNegative (-ve)
CapsuleNon-Capsulated
CatalasePositive (+ve)
Cetrimide TestNegative (-ve)
CitrateNegative (-ve)
CoagulaseNegative (-ve)
FlagellaNon-Flagellated
GasVariable
Gelatin HydrolysisNegative (-ve)
Gram StainingPositive (+ve)
H2SPositive (+ve)
HemolysisWeak Beta hemolysis
IndoleNegative (-ve)
MotilityNon-Motile
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentPositive (+ve)
ShapeRod/Club Shaped
SporeNon-Sporing
UreaseNegative (-ve)
ArabinosePositive (+ve)
DextrosePositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycogenNegative (-ve)
HippurateNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
RaffinoseVariable
RhamnosePositive (+ve)
SalicinPositive (+ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Alkaline PhosphataseNegative (-ve)
Casein HydrolysisNegative (-ve)
Esculin HydrolysisNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
TelluritePositive (+ve)
Tyrosine HydrolysisNegative (-ve)
Biochemical Tests of Pseudomonas aeruginosa Results
CapsuleSome Non-Capsulated and Some are encapsulated
CatalasePositive (+ve)
Cetrimide TestPositive (+ve)
CitratePositive (+ve)
CoagulaseNegative (-ve)
FlagellaFlagellated (Single)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingNegative (-ve)
H2SNegative (-ve)
HemolysisBeta Hemolytic
IndoleNegative (-ve)
MotilityMotile (Unipolar)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Oxidative
OxidasePositive (+ve)
PigmentPositive (+ve) (Blue/Green)
ShapeRod
SporeNon-Sporing
TSIA (Triple Sugar Iron Agar)Alkali/Alkali (Red/Red)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DNaseNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
HippurateNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MalonatePositive (+ve)
MaltoseNegative (-ve)
MannitolPositive (+ve)
MannoseNegative (-ve)
MucateNegative (-ve)
MyoInositolNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TartrateNegative (-ve)
TrehaloseNegative (-ve)
XyloseNegative (-ve)
Acetate UtilizationPositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisNegative (-ve)
LecithinaseNegative (-ve)
LipasePositive (+ve)
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Biochemical Tests of Actinomyces israelii Results
CAMPNegative (-ve)
CapsuleNon-Capsulated
CatalaseNegative (-ve)
FlagellaNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingPositive (+ve)
H2SPositive (+ve)
HemolysisNegative (-ve)
IndoleNegative (-ve)
MotilityNon-Motile
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Anaerobic
PigmentNegative (-ve)
ShapeRod
SporeNon-Sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
CellobiosePositive (+ve)
DNaseNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
GlycogenNegative (-ve)
HippurateNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseVariable
MyoInositolVariable
RaffinosePositive (+ve)
RhamnoseNegative (-ve)
RiboseVariable
SalicinVariable
SorbitolVariable
StarchNegative (-ve)
SucrosePositive (+ve)
TrehaloseVariable
XylosePositive (+ve)
Acid PhosphataseVariable
Alkaline PhosphataseNegative (-ve)
Arginine DihydrolasePositive (+ve)
Casein HydrolysisNegative (-ve)
Esculin HydrolysisPositive (+ve)
LipaseNegative (-ve)
LysinePositive (+ve)
Biochemical Tests of Streptococcus canis Results
CAMPNegative (-ve)
CatalaseNegative (-ve)
Gram StainingPositive (+ve)
OF (Oxidative-Fermentative)Facultative anaerobes
ShapeCocci
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
ArbutinPositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycogenNegative (-ve)
HippurateNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinPositive (+ve)
SorbitolNegative (-ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehaloseVariable
XyloseNegative (-ve)
Alkaline PhosphatasePositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisPositive (+ve)
HyalurodinaseNegative (-ve)
Biochemical Tests for Providencia stuartii Results
CapsuleNon-Capsulated
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaFlagellated
Gas from glucoseNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
Growth in KCNPositive (+ve)
H2SNegative (-ve)
IndolePositive (+ve)
MotilityPositive (+ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporeNon-Sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseNegative (-ve)
DNaseNegative (-ve)
ErythritolNegative (-ve)
Glucose (Acid)Positive (+ve)
GlycerolVariable
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
MucateNegative (-ve)
MyoInositolPositive (+ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
SucroseVariable
TartratePositive (+ve)
TrehalosePositive (+ve)
Acetate UtilizationPositive (+ve)
Aesculine HydrolysisNegative (-ve)
Arginine DihydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
LipaseNegative (-ve)
Lysine DecarboxylaseNegative (-ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminasePositive (+ve)
Biochemical Tests of Hafnia alvei Results
CapsuleNon-Capsulated
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaFlagellated
Gas from GlucosePositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
Growth in KCNPositive (+ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityMotile
MR (Methyl Red)Variable
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRod
SporeNon-Sporing
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)
AdonitolNegative (-ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
Arbutin
DNaseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
InositolNegative (-ve)
LactoseNegative (-ve)
MalonateVariable
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseNegative (-ve)
MucateNegative (-ve)
MyoInositolNegative (-ve)
RaffinoseNegative (-ve)
RhamnosePositive (+ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
SucroseNegative (-ve)
TartratePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Acetate UtilizationNegative (-ve)
Arginine DihydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
LipaseNegative (-ve)
Lysine DecarboxylasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylasePositive (+ve)
Phenylalanine DeaminaseNegative (-ve)
Tryptophan DeaminaseNegative (-ve)
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