Protein Purification Methods
MN Editors
In
Biochemistry

Protein Purification Methods

What is Protein Purification? In biochemistry, a protein is a long chain of amino acids that are all linked together.

Adenosine triphosphate (ATP) Structure, Synthesis, Functions
MN Editors
In
Biochemistry

Adenosine triphosphate (ATP) Structure, Synthesis, Functions

Because the body is complex, energy is required to ensure proper functioning. Adenosine triphosphate, or ATP, is the energy source for use and storage at cellular level. ATP's structure is a nucleoside triophosphate. It consists of a ribose glucose, a nitrogenous base (adenine) and three serially bonded phosphate group. ATP is often referred to by the term "energy currency" because it can be readily releasable in the bond between the third and second phosphate groups. Hydrolysis, which is the process of reducing ATP to energy, serves a wide range of cell functions including signaling and DNA/RNA synthesis. ATP synthesis uses energy from many catabolic mechanisms including cell respiration, betaoxidation, ketosis, and cellular metabolism.

The Laws of Thermodynamics
MN Editors
In
Biochemistry

The Laws of Thermodynamics

The chemical energy stored in molecules can be released as heat in chemical reactions that occur when the fuel methane,

Gibbs Free Energy
MN Editors
In
Biochemistry

Gibbs Free Energy

Gibbs free energy is also referred to as the Gibbs function. Gibbs energy, also known as free enthalpy, is the

What is Entropy?
MN Editors
In
Biochemistry

What is Entropy?

Entropy is among the most important concepts that students should be able to comprehend clearly when learning Chemistry as well as Physics. In addition, entropy may be described in a variety of ways, which means it can be used in a variety of stages or scenarios, like the thermodynamics stage, in cosmic cosmology, or even in economics. The term "entropy" basically refers to the changes that occur spontaneously that take place in everyday phenomena as well as the general tendency to disorder.

Amino Acids Physical Properties, Structure, Classification, Functions
Sourav Bio
In
Biochemistry

Amino Acids Physical Properties, Structure, Classification, Functions

mainly due to their ampholytic properties and biochemically mainly because they are protein constituents. An amino acid is a carboxylic acids that contain an aliphatic primary ammonium group in the same position as the carboxyl group. It also has a distinctive stereochemistry. Biosynthesis of proteins is done using 20 amino acids, which are subject to strict genetic control.

Proteins Definition, Properties, Structure, Classification, Functions
MN Editors
In
Biochemistry

Proteins Definition, Properties, Structure, Classification, Functions

Proteins are the largest macromolecules in biology, found throughout every cell. They are also the most adaptable organic molecule in living system and is found in a many various kinds that range in size from small polymers to huge peptides. Proteins are polymers composed of amino acids, which are connected by peptide bonds. Proteins, the protein building blocks are the naturally occurring twenty amino acids. Proteins are, therefore, the multimers made up of amino acids.

Lineweaver–Burk Plot
MN Editors
In
Biochemistry

Lineweaver–Burk Plot

the presence of an infinite amount of substrate and is therefore impossible to determine Km and Vmax from a hyperbolic graph. Due to this issue the equation of Michaelis-Menten was converted into an equation of straight lines using Lineweaver along with Burk.

Feeder Pathways for Glycolysis
MN Editors
In
Biochemistry

Feeder Pathways for Glycolysis

Numerous carbohydrates, including glucose, meet their catabolic end in $ycolysis after being transformed into glycolytic intermediates. Most significant are glycogen and starch, which are storage polysaccharides that are either in cell walls (endogenous) or in the diet. The disaccharides are maltose. Lactose, trehalose. and sucrose, and the monosaccharides fructose and mannose and galactose.

Fates of Pyruvate
MN Editors
In
Biochemistry

Fates of Pyruvate

Pyruvate is a component of the process of lactic acid and alcohol fermentation in anaerobic conditions. in an aerobic state, pyruvate loses carbon dioxide and hydrogen, and transform into Acetyl CoA and enter the TCA cycle. It can as well, enter the biosynthetic pathways. If there is a drop in levels of glucose, pyruvate goes into the gluconeogenesis process.

Regulation of Glycolysis
MN Editors
In
Biochemistry

Regulation of Glycolysis

Glycolysis is the primary stage in the process of breaking down glucose in order to obtain energy to power the cell's metabolism. The majority of living organisms perform glycolysis as a part in their metabolic process. This process does not require oxygen, which is why it is considered anaerobic. Glycolysis happens in the cytoplasms of both eukaryotic as well as prokaryotic cells. 

Post-glycolysis processes
MN Editors
In
Biochemistry

Post-glycolysis processes

Glycolysis could not continue indefinitely if all NAD+ was used up and glycolysis would cease. Organisms must be capable of oxidizing NADH back into NAD+ in order to allow glycolysis to continue. The external electron acceptor will determine how this is done.

Carbohydrates Definition, Examples, Structure, Types, and Functions
MN Editors
In
Biochemistry

Carbohydrates Definition, Examples, Structure, Types, and Functions

The term "carbohydrate" comes from the Greek word sakharon meaning "sugar". In the field of chemistry, carbohydrates comprise the most common type of organic compounds with simple structures. A carbohydrate is an aldehyde , or one that contains other hydroxyl groups. The most basic carbohydrates are known as monosaccharides. They have the fundamental structure (C*H2O)n where 3 or more is the minimum.

Lipids Definition, Structure, Properties, types, Examples, and Functions
MN Editors
In
Biochemistry

Lipids Definition, Structure, Properties, Types, Examples, and Functions

It is common to discuss fat as if it was a villainous substance that is bent to destroy our diets. However, they are beautiful tiny molecules composed of three hydrocarbon tails that are attached to a small coathanger-like molecule known as Glycerol. Similar to the other big organic molecules they perform crucial functions in the biology of both humans and other living things. (Also numerous recent diet studies suggest that sugar can cause many more health issues that fat!)

The Wobble Hypothesis
MN Editors
In
Biochemistry

The Wobble Hypothesis

According to this hypothesis, only the first two bases of the codon have a precise pairing with the bases of the anticodon of tRNA, while the pairing between the third bases of codon and anticodon may Wobble (wobble means to sway or move unsteadily).

Mitochondrial Shuttles
MN Editors
In
Biochemistry

Mitochondrial Shuttles and Transporter Proteins.

Mitochondrial transporter The mitochondria contain different types of transporter proteins within the intermembrane. These transporters transfers ADP, Pi, and H

Electron Transport Chain
MN Editors
In
Biochemistry

Electron Transport Chain Definition, Steps and ATP synthase.

The Electron Transport Chain is made of different protein complexes which perform a redox reaction to transfer electrons from electron donor to electron acceptor and also perform the transfer of protons from matrix to intermembrane space.