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Stokes Disc Diffusion Method – Principle, Procedure, Result.

Stokes Disc Diffusion Method Principle, Procedure, Result.

Stokes disc diffusion method isn’t as well-standardized as Kirby-Bauer’s method and is utilized in labs especially where the exact amount of antimicrobial present in discs isn’t known because of the difficulty in getting discs and correctly storing them or when other requirements needed for the Kirby-Bauer method cannot be fulfilled.

Most Probable Number (MPN) Test – Principle, Procedure, Results

Most Probable Number (MPN) Test: Principle, Procedure, Results

What is Most Probable Number (MPN) Test? The Most Probable Number (MPN) test is a widely recognized method employed to determine the concentration of viable microorganisms in a given sample. This method involves the use of liquid broth growth in ten-fold dilutions to replicate and estimate microbial populations. It is especially beneficial for samples like … Read more

Lysine Iron Agar (LIA) Test Principle, Procedure, Result

Lysine Iron Agar (LIA) Test Principle, Procedure, Result

What is the Lysine Iron Agar (LIA) Test? The solid medium Lysine Iron Agar (LIA) is indicated for use in qualitative techniques for the differentiation of microorganisms based on the generation of lysine decarboxylase and hydrogen sulphide. Edwards and Fife created Lysine Iron Agar to identify lactose-fermenting Salmonellae. Salmonellae are known to rapidly decarboxylate lysine … Read more

Types of Blood Collection Tubes and Their uses

Types of Blood Collection Tubes and Their uses

Evacuated Tubes that are used for blood collection for different laboratory tests are made up of tubes in variable sizes, and color-coded tops to indicate tube contents. The majority of blood collection tubes contain an ingredient that either speeds up the blood’s clotting (clot activator) or stops blood from becoming clot-free (anticoagulant). The following list lists the most frequently utilized blood collection tubes, along with their additives, and their uses in lab:

Nagler Reaction – Lecithinase Test

Nagler Reaction - Lecithinase Test

Nagler’s Reaction or Lecithinase test is a test in biochemistry used to detect organisms that produce the phospholipases (lecithinases) e.g. Clostridium perfringens. Its alpha (a) toxin from C. perfringens exhibits the phospholipase enzyme activity, which aids in the distinction between C. perfringens and the other Clostridium species that generate the enzyme lecithinase (C.baratti, C.absonum, C.bifermantans, C.sordelli, and C.novyi) through neutralization of lecithin C activity with an antitoxin.

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