Microinjection – Definition, Types, Principle, Steps Applications

Microinjection - Definition, Types, Principle, Steps Applications

What is Microinjection? Definition of Microinjection Microinjection is a precise technique used to introduce DNA or other genetic materials directly into a cell using a micropipette or fine glass needle. It is widely employed in genetic engineering, genome editing, and various biomedical research areas. Principle of Microinjection Microinjection operates on the fundamental principle of directly … Read more

Pseudomonas Aeruginosa on Macconkey Agar

Pseudomonas Aeruginosa on Macconkey Agar

Pseudomonas Aeruginosa Principle The MacConkey Agar is a specialized culture medium widely used in microbiology for the isolation, identification, and differentiation of gram-negative bacteria, particularly those belonging to the Enterobacteriaceae family. The principle of MacConkey Agar lies in its combination of selective and differential properties, making it an effective tool in microbiological analyses. The medium’s … Read more

How to Use a Micropipette?

How to Use a Micropipette?

Standard laboratory equipment for measuring and transferring minute amounts of liquid includes micropipettors. You’ll put them to use all through the semester and into subsequent upper-level courses. If you want reliable results from your tests, you must become an expert user of these tools. Objective Parts of a micropipette Different sizes of micropipettes The laboratory’s … Read more

Air Displacement Pipetting – Principle, Procedure, Applications

Operation of Air displacement Pipette

An air displacement pipette (also known as a “volumetric pipette” or “mechanical pipette”) is a type of liquid handling tool commonly used in laboratory settings. It uses air displacement to draw a specific volume of liquid into the pipette’s barrel. The volume of liquid is determined by the calibrated volume markings on the barrel of … Read more

Positive Displacement Pipette – Principle, Procedure, Applications, Advantages

Positive Displacement Pipetting technique

Positive displacement pipettes are characterised by piston-driven displacement. The piston in a positive displacement pipette makes direct contact with the sample, which allows the aspiration force to remain constant. Air displacement pipettes accurately dispense the majority of liquids. However, the precision may be affected negatively by the solution’s viscosity, volatility, surface tension, and temperature. For … Read more

Pipetting – Technique, Definition, Types

Pipetting - Technique, Definition, Types

Pipetting Definition Pipetting is the process of using a pipettor to measure and dispense small volumes of liquid. It is a fundamental technique used in many laboratory procedures, including preparing solutions, dispensing samples for analysis, and performing various types of assays. When pipetting, the user first sets the desired volume on the pipettor by adjusting … Read more

Filtration – Definition, Mechanisms, Types, Examples, Application

Gravity filtration

What is Filtration? Filtration is utilised to separate a dispersion based on particle size. The filtered mixture is put to an appropriate porous material filter. Particles smaller than the filter’s pores can pass through and enter the filtrate, while larger particles are retained on the filter’s surface. Filtration is a process that is used to … Read more

How to balance a centrifuge? – Centrifuge Balancing

How to balance a centrifuge? - Centrifuge Balancing

What is Centrifuge? What is Centrifuge Balancing? Why it is important to balance a centrifuge? – Why is centrifuge balanced? What Happens if a Centrifuge is Unbalanced?  How Centrifuge Works? What is Centrifuge Tube? How to balance in different  centrifuge? If you are utilising a portable benchtop instrument, you must ensure that the centrifuge is … Read more

Isolation of Microorganisms from Soil

Isolation of Microorganism from Soil

Soil contain different types of microorganisms which are listed below; Bacteria: Bacteria is the key soil workforce. They are the last stage in the process of breaking down nutrients before releasing them into the root zone of the plant. In fact, they are the final stage of breaking down nutrients. Food and Agriculture Organization once … Read more

Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs

Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs

Microorganisms are everywhere in their the distribution. In any given environment, many microorganisms are present at any given time. It is difficult to identify a specific kind of microbe until we understand their ecological needs. For instance, anaerobic microbes do not require oxygen those that are aerobic require oxygen micro-aerophiles to have oxygen however, in a small amount. So, we require cotton plugs to provide an conditions that are aerobic and keep the growing culture uninvolved with unwanted microbes. Cotton plugs are made of cotton along with air pours. Air can flow through air pours, but not the bacteria that cause microbial contamination. They are fixed to the surface of cotton fibres , and prevent them from being able from getting into flasks, tubes, etc. Air aids in for the development of microorganisms within glass equipment.

Method for Balancing in Laboratory – Weight or Mass Measurement

Method for Balancing in Laboratory - Weight or Mass Measurement

The most frequent tasks in the microbiology area is the determination of the weight or mass of desired substances, chemicals. Another important aspect to be considered is the preservation of substances. Thus, the powders, as well as other granular or paste-like substances should Chamber not be placed directly on the platform for weighing of the balance. It is recommended that glazed paper or a small weigh boats is the best choice to weigh. It is recommended to use glazed papers if the material is 15 grams or less needs to be weighed. A the weigh boat pan or small beaker must be utilized if you are weighing a larger amount. Because of the the light weight of glazed papers its weight is subject to a minimal. If the weight is less of 1 gram an electronic balance is recommended. The larger amount (above 1 grams) is not to be considered using electronic balances.

Different Aseptic Techniques in Microbiology Laboratory

Different Aseptic Techniques in Microbiology Laboratory

Because the aim of biologists is to cultivate microorganisms or eukaryotic cells with no introduction of any extraneous organisms aseptic methods are essential for ensuring that experiments are conducted with precision and accuracy. Always keep in mind that a totally safe working environment for scientists does not exist.

Moist Heat Sterilization Principle, Advantages, Disadvantages

Moist Heat Sterilization Principle, Advantages, Disadvantages

Of all the options to sterilize (killing or eliminating all microorganisms, which includes the spores of bacterial growth) moist heat that is saturated steam with pressure has been the most frequently utilized and most reliable method. Moisture has more penetrating power than dry heat, and at a certain temperature, causes a greater reduction in the quantity of live microorganisms. Steam sterilization is safe cheap, low-cost, quickly microbicidal, as well as sporicidal. It quickly heats and penetrates into fabrics.

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