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Microbiology Techniques
How to Use a Micropipette?
Standard laboratory equipment for measuring and transferring minute amounts of liquid includes micropipettors. You’ll put them to use all through
Microbiology Techniques
Microbiology techniques are methods used by microbiologists to study microorganisms, including bacteria, viruses, fungi, and parasites. These techniques are used to identify, cultivate, and analyze microorganisms in order to understand their biology, ecology, and potential impact on human health and the environment.
Here are some examples of microbiology techniques:
- Microscopy: Microscopy involves the use of a microscope to view microorganisms. Different types of microscopes, including compound microscopes, electron microscopes, and fluorescence microscopes, can be used to study microorganisms at different scales and with different levels of detail.
- Culture techniques: Culture techniques involve the cultivation of microorganisms in the laboratory. This can be done using various media, including solid and liquid media, and can be used to study the growth, metabolism, and genetics of microorganisms.
- Molecular techniques: Molecular techniques involve the analysis of the genetic material of microorganisms, including DNA and RNA. These techniques, such as DNA sequencing and PCR (polymerase chain reaction), can be used to identify and classify microorganisms, and to study their genetic makeup and evolution.
- Biochemical techniques: Biochemical techniques involve the analysis of the metabolic products and enzymes of microorganisms. These techniques, such as enzyme-linked immunosorbent assays (ELISAs) and biochemical tests, can be used to identify and classify microorganisms and to study their metabolism and physiology.
Overall, microbiology techniques are essential for understanding the biology of microorganisms and their roles in various environments and in human health.
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Microbiology Techniques
Air Displacement Pipetting – Principle, Procedure, Applications
An air displacement pipette (also known as a “volumetric pipette” or “mechanical pipette”) is a type of liquid handling tool
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Microbiology Techniques
Positive Displacement Pipette – Principle, Procedure, Applications, Advantages
Positive displacement pipettes are characterised by piston-driven displacement. The piston in a positive displacement pipette makes direct contact with the
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Microbiology Techniques
What is Ergonomic Pipetting?
What is Ergonomic Pipetting? Ergonomic pipetting is the design and use of laboratory pipettes in a way that reduces the
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Microbiology Techniques
Pipetting – Technique, Definition, Types
Pipetting Definition Pipetting is the process of using a pipettor to measure and dispense small volumes of liquid. It is
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Microbiology Techniques
Filtration – Definition, Mechanisms, Types, Examples, Application
What is Filtration? Filtration is utilised to separate a dispersion based on particle size. The filtered mixture is put to
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Microbiology Techniques
How to balance a centrifuge? – Centrifuge Balancing
What is Centrifuge? What is Centrifuge Balancing? Why it is important to balance a centrifuge? – Why is centrifuge balanced?
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Microbiology Techniques
How To Use A Microscope Properly
Carry the microscope close to your body, with one hand on the arm and the other on the base, when
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Microbiology Techniques
Isolation of Microorganisms from Soil
Soil contain different types of microorganisms which are listed below; Bacteria: Bacteria is the key soil workforce. They are the
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Microbiology Techniques
Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs
Microorganisms are everywhere in their the distribution. In any given environment, many microorganisms are present at any given time. It is difficult to identify a specific kind of microbe until we understand their ecological needs. For instance, anaerobic microbes do not require oxygen those that are aerobic require oxygen micro-aerophiles to have oxygen however, in a small amount. So, we require cotton plugs to provide an conditions that are aerobic and keep the growing culture uninvolved with unwanted microbes. Cotton plugs are made of cotton along with air pours. Air can flow through air pours, but not the bacteria that cause microbial contamination. They are fixed to the surface of cotton fibres , and prevent them from being able from getting into flasks, tubes, etc. Air aids in for the development of microorganisms within glass equipment.
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Microbiology Techniques
Method for Balancing in Laboratory – Weight or Mass Measurement
The most frequent tasks in the microbiology area is the determination of the weight or mass of desired substances, chemicals. Another important aspect to be considered is the preservation of substances. Thus, the powders, as well as other granular or paste-like substances should Chamber not be placed directly on the platform for weighing of the balance. It is recommended that glazed paper or a small weigh boats is the best choice to weigh. It is recommended to use glazed papers if the material is 15 grams or less needs to be weighed. A the weigh boat pan or small beaker must be utilized if you are weighing a larger amount. Because of the the light weight of glazed papers its weight is subject to a minimal. If the weight is less of 1 gram an electronic balance is recommended. The larger amount (above 1 grams) is not to be considered using electronic balances.
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Microbiology Techniques
Different Aseptic Techniques in Microbiology Laboratory
Because the aim of biologists is to cultivate microorganisms or eukaryotic cells with no introduction of any extraneous organisms aseptic methods are essential for ensuring that experiments are conducted with precision and accuracy. Always keep in mind that a totally safe working environment for scientists does not exist.
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Microbiology Techniques
Moist Heat Sterilization Principle, Advantages, Disadvantages
Of all the options to sterilize (killing or eliminating all microorganisms, which includes the spores of bacterial growth) moist heat that is saturated steam with pressure has been the most frequently utilized and most reliable method. Moisture has more penetrating power than dry heat, and at a certain temperature, causes a greater reduction in the quantity of live microorganisms. Steam sterilization is safe cheap, low-cost, quickly microbicidal, as well as sporicidal. It quickly heats and penetrates into fabrics.
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Microbiology Techniques
Coagulase Test Principle, Procedure, Application, Result.
It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).
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Microbiology Techniques
Counter Current Immunoelectrophoresis Principle, Procedure, Result.
Counter current immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody migrate towards opposite directions and form a visible white precipitate in the area between the wells.
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Microbiology Techniques
Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.
Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.
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Microbiology Techniques
Immunoelectrophoresis test Principle, Applications, Procedure, Results, Advantages and Disadvantages.
Immunoelectrophoresis is a powerful qualitative technique for the characterization of an antibody.
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Microbiology Techniques
Serial Dilution Method Definition, Procedure, Application
It is routine practise to quantify microbial counts for both liquid and solid specimens, including E. coli suspensions in nutritional
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Microbiology Techniques
Subculturing technique for Broth Culture
Aim Transfer of microbial cells from a parent culture medium to a fresh broth culture. Principle The broth is a
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Microbiology Techniques
Subculturing Technique In Agar Slant/Agar Plate
Overview of Subculturing Technique In microbiology, subculture is defined as a new cell or microbiological culture, which is prepared by
