Get Study notes related to practicals of microbiology.
A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae.
Agarose gel electrophoresis (AGE) is an approach that is used to distinguish DNA from RNA based on their molecular sizes. The separation of RNA and DNA molecules is accomplished when nucleic acids that are negatively charged travel through an agarose structure under an influence of an electrical fields (electrophoresis).
Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.
A compound microscope is a class of optical or light microscope. I
The term Giemsa stain originated from a name of German chemist and
A blood film or Peripheral blood smear (PBS) must be prepared from
A microtome is a scientific instrument used to cut very thin slices
What is Pauly’s Test? Purposes of Pauly’s Test Principle of Pauly’s Test
What is Reticulocytes? Reticulocytes are immature red blood cells (erythrocytes) that have
What is Hematoxylin and Eosin (H&E) Staining? Hematoxylin and Eosin (H&E) staining
The majority of plasma proteins are too big to pass through the
The bulk of plasma proteins are composed of big particles, making it
The lactophenol cotton blue (LPCB) wet mount preparation is the most used
Trichrome stains, such as Masson’s Trichrome, derive its name from the three
Since the late 1950s and early 1960s, the positive staining approach has
What is Wheatley Trichrome Staining? In parasitology, intestinal protozoans are typically detected
Romanowsky staining, also known as Romanowsky–Giemsa staining, is a classic staining technique
