Decarboxylase Test Principle, Procedure, Results, Uses
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Culture Media

Decarboxylase Broth Protocol

Moeller introduced the decarboxylase media to detect the ability of bacteria to decarboxylate amino acid. They were used for the first time to detect lysine, ornithine, and arginine decarboxylases among Enterobacteriaceae bacteria.

Salt Meat Broth
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Culture Media

Salt Meat Broth

Salt Meat Broth is used as an enrichment medium for staphylococci isolation from severely contaminated specimens.

Burkholderia Cepacia Agar Base Preparation
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Culture Media

Burkholderia Cepacia Agar Base Preparation, Composition, Principle

Burkholderia cepacia can be viewed as an opportunity-based bacterium that is associated with nosocomial illnesses caused by contamination of medical equipment and disinfectants. However, the highest risk group is CF patients. People with cystic fibrosis are at an increased risk of infection. those with the infection, if not treated are prone to rapid declines of lung functions, frequent bacteremia and even death due to lung dysfunction.

Classification of Culture Media
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Culture Media

Classification of Culture Media

When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn't be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.

Preparation of Solid Media - agar deep tubes, Agar Slants, Plates
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Culture Media

Preparation of Solid Media – Agar deep tubes, Agar Slants, Plates

If the broth medium is supplemented with agar-agar it is referred to as agar medium like the nutrient Agar Medium (NAM) for the cultivation of bacteria potato dextrose (PDA) medium for the cultivation and storage of general fungi Czapek Dox agar (CDA) medium for fungi, starch-casein (SCA) media for the cultivation of actinomycetes, and so on.

Lysogeny broth (LB) Preparation
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Culture Media

Lysogeny broth (LB) Preparation

LB is the most widely used bacterial culture medium but its roots lie in the area of genetics of bacteriophage. Guiseppi Bertani developed the LB recipe when he was trying to increase the amount of plaque that formed on an indicator strain of Shigella (Bertani 1952). As per Bertani, LB has been often misinterpreted to mean "Luria Broth", "Luria-Bertani" medium, or "Lennox Broth"; however the original acronym stood as "Lysogeny Broth" (Bertani, 2004). The agar version of the medium is identified as LA however, it is frequently called LB. While initially developed to study bacteriophage in addition to Shigella growth, LB subsequently became the most preferred medium to grow Escherichia bacteria and other species of the enteric.

Preparation of Liquid Medium/broth
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Culture Media

Preparation of Liquid Medium/broth

The process of growing an organism in the surface of a medium is known as culture. The food source that supports the development of the organism is known as a culture medium. The media for culture are designed so that the organism will receive all the nutrition requirements. However, the media for culture are made in laboratories by weighing and dispensing particular ingredients or by purchasing ready-made media on the market. The majority of the media include organic as well as inorganic nutrients. However, to cultivate a variety of microorganisms, special media are created. If the media is to be solidified required, agar-agar can be mixed with other ingredients. The culture media can be classified into three categories that include semi-synthetic, natural and synthetic media each of which is employed for microbiological studies.

Bird Seed Agar Composition, Principle, Preparation, Results, Uses, Limitations
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Culture Media

Bird Seed Agar Composition, Principle, Preparation

Bird Seed Agar Bird Seed Agar is an effective solid medium to isolate selectively and differentially of Cryptococcus Neoformans from clinical specimens. It is utilized for the specific isolation from Cryptococcus neoformans as well as C. Gattii. C. Neoformans typically grows as yeast (unicellular) and reproduces via budding. Cryptococcus Neoformans is an enclosed yeast, which makes the enzyme phenoloxidase an enzyme that is essential to melanin production. 

Brucella Agar Composition, Principle, Preparation, Results, Uses
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Culture Media

Brucella Agar Composition, Principle, Preparation, Results, Uses

Brucella is an intracellular parasite which causes epizootic abortions for animals, and septicemic febrile disease or localized infections of tissues, bones or organ systems of humans. Brucella species are very meticulous and require a rich nutrient environment to develop. Additionally, Brucella species are highly infectious, and therefore extreme caution should be exercised when handling.

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Culture Media

Ashdown’s Agar Composition, Principle, Preparation, Results, Uses

Ashdown's medium can be described as a selective culture medium that allows for the identification and characterisation of Burkholderia pseudomallei (the bacteria which causes the disease melioidosis). The Ashdown's Medium was initially identified by LR Ashdown in 1979.

VRBA Composition, Principle, Preparation, Results, Uses
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Culture Media

VRBA Composition, Principle, Preparation, Results, Uses

Coliforms are all facultative and aerobic rod-shaped, gram-negative, non-spore-forming bacteria that produce lactose by fermentation with gas and acid production within 48 hours, at temperatures of 35 degrees Celsius. Methods to identify, enumerate and presumedly identify coliforms are utilized in the testing of dairy and food products. One method of performing the presumptive test to identify coliforms employs the Violet Red Bile Agar (VRBA). The Violet Red Bile Aggar (VRBA) is an selec-tive medium that can be used to detect and quantify lactose-fermenting coliform microorganisms.

Tryptic Soy Agar Composition, Principle, Preparation, Results, Uses
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Culture Media

Tryptic Soy Agar (TSA) Composition, Principle, Preparation, Results, Uses

Tryptic Soy Agar can be used to grow microorganisms. These media are non-selective and general-purpose. They provide enough nutrients to support a variety of microorganisms. This medium can also be used to grow, store, maintain, and transport pure microorganism cultures.

Thioglycollate Broth Composition, Principle, Preparation, Results, Uses
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Culture Media

Thioglycollate Broth Composition, Principle, Preparation, Results, Uses

Thioglycolate broth, an enrichment broth that is multipurpose and can be used to determine the oxygen needs of microorganisms, is called a differential medium. It is used most often in diagnostic bacteriology as an enrichment broth. This broth is supportive of the growth and development of microorganisms fastidious, anaerophilic, microaerophilic and aerobes.

Amies Transport Medium Composition, Principle, Preparation, Results, Uses
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Culture Media

Amies Transport Medium Composition, Principle, Preparation, Results, Uses

Amies Transport Medium, which contains charcoal to increase the viability and longevity of pathogenic organisms, is an improved transport medium. It is semisolid media that can be used in qualitative procedures to transport clinical swab specimens from the hospital to the laboratory. This modified Stuart's Transport Medium is made by adding charcoal to the medium and replacing the glycerophosphate. This modified medium produced a higher percentage positive results than Stuart's transport medium.