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Robertson’s Cooked Meat Medium (RCM Medium) Preparation, Composition.
MN Editors
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Culture Media

Robertson’s Cooked Meat Medium (RCM Medium) – Composition, Preparation, Uses

Cooked Meat Medium was originally developed by Robertson to cultivate specific anaerobes isolated from wounds. The current formulation is a modified version, sometimes referred to by the name of Chopped Meat Medium, which allows for the growth of a variety of spores and non-spore-forming strict anaerobes.

Transport Media Used in Laboratories
MN Editors
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Culture Media

Transport Media – Definition, Types, Principle, Uses, Examples

Transport media are basically buffer solutions that contain carbohydrate, peptones, along with other nutritional elements (excluding growing factors) that are designed to protect the health of bacteria during transport, without allowing for their multiplicity. The main goal of utilization of the medium is to keep the sample as close to its original condition as is possible.

Decarboxylase Test Principle, Procedure, Results, Uses
MN Editors
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Culture Media

Decarboxylase Broth Protocol

Moeller introduced the decarboxylase media to detect the ability of bacteria to decarboxylate amino acid. They were used for the first time to detect lysine, ornithine, and arginine decarboxylases among Enterobacteriaceae bacteria.

Salt Meat Broth
MN Editors
In
Culture Media

Salt Meat Broth

Salt Meat Broth is used as an enrichment medium for staphylococci isolation from severely contaminated specimens.

Burkholderia Cepacia Agar Base Preparation
MN Editors
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Culture Media

Burkholderia Cepacia Agar Base Preparation, Composition, Principle

Burkholderia cepacia can be viewed as an opportunity-based bacterium that is associated with nosocomial illnesses caused by contamination of medical equipment and disinfectants. However, the highest risk group is CF patients. People with cystic fibrosis are at an increased risk of infection. those with the infection, if not treated are prone to rapid declines of lung functions, frequent bacteremia and even death due to lung dysfunction.

Classification of Culture Media
MN Editors
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Culture Media

Classification of Culture Media

When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn't be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.

Preparation of Solid Media - agar deep tubes, Agar Slants, Plates
MN Editors
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Culture Media

Preparation of Solid Media – Agar deep tubes, Agar Slants, Plates

If the broth medium is supplemented with agar-agar it is referred to as agar medium like the nutrient Agar Medium (NAM) for the cultivation of bacteria potato dextrose (PDA) medium for the cultivation and storage of general fungi Czapek Dox agar (CDA) medium for fungi, starch-casein (SCA) media for the cultivation of actinomycetes, and so on.

Lysogeny broth (LB) Preparation
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Culture Media

Lysogeny broth (LB) Preparation

LB is the most widely used bacterial culture medium but its roots lie in the area of genetics of bacteriophage. Guiseppi Bertani developed the LB recipe when he was trying to increase the amount of plaque that formed on an indicator strain of Shigella (Bertani 1952). As per Bertani, LB has been often misinterpreted to mean "Luria Broth", "Luria-Bertani" medium, or "Lennox Broth"; however the original acronym stood as "Lysogeny Broth" (Bertani, 2004). The agar version of the medium is identified as LA however, it is frequently called LB. While initially developed to study bacteriophage in addition to Shigella growth, LB subsequently became the most preferred medium to grow Escherichia bacteria and other species of the enteric.

Preparation of Liquid Medium/broth
Sourav Bio
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Culture Media

Preparation of Liquid Medium/broth

The process of growing an organism in the surface of a medium is known as culture. The food source that supports the development of the organism is known as a culture medium. The media for culture are designed so that the organism will receive all the nutrition requirements. However, the media for culture are made in laboratories by weighing and dispensing particular ingredients or by purchasing ready-made media on the market. The majority of the media include organic as well as inorganic nutrients. However, to cultivate a variety of microorganisms, special media are created. If the media is to be solidified required, agar-agar can be mixed with other ingredients. The culture media can be classified into three categories that include semi-synthetic, natural and synthetic media each of which is employed for microbiological studies.

Bird Seed Agar Composition, Principle, Preparation, Results, Uses, Limitations
MN Editors
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Culture Media

Bird Seed Agar (Staibs Medium) Composition, Principle, Preparation

Bird Seed Agar Bird Seed Agar is an effective solid medium to isolate selectively and differentially of Cryptococcus Neoformans from clinical specimens. It is utilized for the specific isolation from Cryptococcus neoformans as well as C. Gattii. C. Neoformans typically grows as yeast (unicellular) and reproduces via budding. Cryptococcus Neoformans is an enclosed yeast, which makes the enzyme phenoloxidase an enzyme that is essential to melanin production. 

Brucella Agar Composition, Principle, Preparation, Results, Uses
MN Editors
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Culture Media

Brucella Agar Composition, Principle, Preparation, Results, Uses

Brucella is an intracellular parasite which causes epizootic abortions for animals, and septicemic febrile disease or localized infections of tissues, bones or organ systems of humans. Brucella species are very meticulous and require a rich nutrient environment to develop. Additionally, Brucella species are highly infectious, and therefore extreme caution should be exercised when handling.