Real-Time PCR Principle, Protocol, Application, Advantages.
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Molecular biology

Real Time PCR (RT-PCR) – Definition, Principle, Protocol, Application, Advantages

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

Post-transcriptional modification
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Molecular biology

Post-transcriptional Modification – Definition, Types, Process, Importance

Post-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an primary RNA transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule that can then leave the nucleus and perform any of a variety of different functions in the cell.

DNA Microarray Principle, Types and Steps involved in cDNA microarrays
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Molecular biology

DNA Microarray Principle, Types and Steps involved in cDNA microarrays

The DNA microarray technology is among the most effective technology that can offer an extremely high-throughput and precise overview of the whole transcriptome and genome, which lets scientists understand the molecular processes that underlie natural and malfunctioning biological processes. Microarray technology may accelerate the process of screening thousands of protein and DNA samples at once.

Eukaryotic Transcription
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Molecular biology

Eukaryotic Transcription

Although the procedure of synthesis of RNA is the same in prokaryotes as well as eukaryotes however, the process is much more complicated in eukaryotes. In eukaryotes it is possible to synthesize RNA in the nucleus.

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