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Biochemical Test
Ornithine Decarboxylase Test – Principle, Procedure, Result
What is the ornithine decarboxylase test? Objectives of Decarboxylase Test Principle of
Biochemical Test
Biochemical tests are laboratory procedures that use specific chemical reactions to identify and characterize microorganisms, such as bacteria. These tests are often used to identify the presence of specific enzymes or metabolic pathways in a microorganism, which can help to distinguish it from other microorganisms.
Some common biochemical tests used in bacteriology include:
- Oxidase test: This test is used to detect the presence of the enzyme cytochrome oxidase, which is involved in the metabolism of oxygen.
- Catalase test: This test is used to detect the presence of the enzyme catalase, which helps to break down hydrogen peroxide.
- Indole test: This test is used to detect the presence of the enzyme tryptophanase, which breaks down the amino acid tryptophan.
- Nitrate reduction test: This test is used to detect the ability of a microorganism to reduce nitrate to nitrite.
- Gelatinase test: This test is used to detect the ability of a microorganism to produce the enzyme gelatinase, which breaks down gelatin.
These are just a few examples of the many different biochemical tests that are used in bacteriology. Biochemical tests can be useful for identifying and classifying bacteria, and for understanding their metabolism and ecological role.
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Biochemical Test
Ames Test – Principle, Procedure, Result, Limitation, Applications
What is Ames Test? Definition of Ames Test The Ames test is
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Biochemical Test
Nylander’s Test for Carbohydrates Principle, Procedure, Result
What is Nylander’s Test? Nylander’s Test, a renowned chemical assay, is primarily
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Biochemical Test
Methyl Red (MR) Test – Principle, Procedure, Results, Uses
What is Methyl Red (MR) Test? Objectives of Methyl Red (MR) Test The
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Biochemical Test
Benedict’s Test – Principle, Reagent Preparation, Procedure, Result, Limitation
Benedict's test is a chemical test that is used to test for the presence of reduced sugars within an analytical test. Thus, simple carbohydrates that contain an aldehyde or free ketone functional group are detected using this test. The test is basing itself upon Benedict's Reagent (also called Benedict's solution) which is a complex mix of sodium carbonate, sodium citrate, and the pentahydrate of copper(II) Sulfate.
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Biochemical Test
Indole Test – Principle, Procedure, Result, Uses
What is Indole test? Indole Test Definition The indole test is a
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Biochemical Test
Spot Indole Test – Principle, Procedure, Result, Uses
What is Spot Indole Test? Definition of Spot Indole Test The Spot
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Biochemical Test
Voges Proskauer (VP) Test – Principle, Procedure, Results
In 1898, Voges as well as Proskauer (16)characterized the process of fermentation of sugars by a variety of bacteria. They demonstrated that the gas that was produced during the process was a mixture of H2 and CO2 that when they added KOH to the cultures been grown in glucose peptone medium to allow for a prolonged period of incubation in presence of oxygen certain organisms developed a red fluorescent color. While the exact nature of the color was not known in the early days, this technique was suggested to differentiate between the bacterial strains that produced it and ones that didn't. in 1906 Arthur Harden analyzed the fermentation products of Enterobacter aerogenes. He discovered that when it was surrounded by glucose, the organism made two compounds, acetoin as well as 2,3-butanedio.
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Biochemical Test
Biochemical Test of Yersinia pestis
What is Yersinia pestis? Yersinia pestis is a bacterium responsible for causing
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Biochemical Test
Beta (β) Lactamase Test Principle, Procedure, Results
Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae.
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Biochemical Test
Pauly’s Test – Definition, Principle, Procedure, Uses
What is Pauly’s Test? Purposes of Pauly’s Test Principle of Pauly’s Test
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Biochemical Test
Sulphosalicylic Acid Test for Proteinuria: Principle, Procedure, Result, Uses
The majority of plasma proteins are too big to pass through the
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Biochemical Test
Heat and Acetic Acid Test for Proteinuria: Principle, Procedure, Result, Uses
The bulk of plasma proteins are composed of big particles, making it
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Biochemical Test
Bacteriological Examination of Waters by Using Membrane Filtration Method
Human pathogenic bacteria transmitted via the fecal-oral route, i.e., primarily intestinal pathogens,
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Biochemical Test
Glucose Tolerance Test – Principle, Procedure, Types, Applications
What is a Glucose Tolerance Test? A glucose tolerance test is a
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Biochemical Test
COD Test – Test for Chemical Oxygen Demand In Wastewater
After wastewater treatment, the chemical oxygen demand (COD) is used to determine
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Biochemical Test
BOD Test – Biological Oxygen Demand Test
All aquatic organisms depend on the oxygen in the water (dissolved oxygen)
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Biochemical Test
Coliforms Presence/Absence (PA) Test
What is Presence/Absence Test? The availability of potable water for bathing, drinking,
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Biochemical Test
Fructose Metabolism Pathway – Fructolysis
The main sources of fructose in the diet are the disaccharide sucrose
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Biochemical Test
Gram-Sure (L-Alanine-7-amido-4-methylcourmarin) Test
The Gram stain is typically the initial step in identifying bacteria and
