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MCQ on CRISPR

Molecular Biology MCQ – MCQ on CRISPR 1. What does the cas9 protein do? Prevents viruses from entering bacteria Cut RNA Cut DNA Binds to ...

Molecular Biology MCQ – MCQ on CRISPR

1. What does the cas9 protein do?

  • Prevents viruses from entering bacteria
  • Cut RNA
  • Cut DNA
  • Binds to RNA

[expand title=”Show Answer”]Answer: Cut DNA
[/expand]

2. How does the guide RNA help the cell know where to cut the DNA?

  • Guide RNA helps to find the PAM
  • Guide RNA is complementary to the DNA sequence
  • Guide RNA always binds to the same sequence of DNA
  • Like restriction sites, they are always palindromes

[expand title=”Show Answer”]Answer: Guide RNA is complementary to the DNA sequence
[/expand]

3. What is the role of the guide RNA?

  • It helps the cell repair the DNA
  • ensures that the Cas9 enzyme cuts at the right point in the genome
  • Recognizes PAM sequences and opens up the DNA
  • makes a cut across both strands of the DNA

[expand title=”Show Answer”]Answer: ensures that the Cas9 enzyme cuts at the right point in the genome
[/expand]

4. A PAM is

  • a 15-20 bp sequence snipped from a viral genome that serves as a tracer DNA to locate viruses later
  • a 3 bp sequence that adjacent to viral DNA in a CRISPR array
  • a girl’s name popular in the 1960s
  • where the DNA is cut

[expand title=”Show Answer”]Answer: a 3 bp sequence that adjacent to viral DNA in a CRISPR array
[/expand]

5. Why do bacteria have CRISPR? Check all that apply.

  • To be able to remember which viruses they have been infected with
  • To be able to edit virus DNA
  • To cut apart virus DNA thereby stopping the virus infection
  • To be able to edit their own DNA

[expand title=”Show Answer”]Answer:
To be able to remember which viruses they have been infected with,
To cut apart virus DNA thereby stopping the virus infection
[/expand]

6. CRISPR can edit DNA. What organisms naturally has CRISPR?

  • Viruses
  • Humans
  • Plants
  • Bacteria

[expand title=”Show Answer”]Answer:
Bacteria
[/expand]

7. What does the acronym CRISPR stand for?

  • Controlled Reservative Image Spectacle Palindromic Recounts
  • Clustered Regularly-Interspaced Short Palindromic Repeats

[expand title=”Show Answer”]Answer:
Clustered Regularly-Interspaced Short Palindromic Repeats
[/expand]

8. CRISPR-Cas9 is a type of __ system found in bacteria

  • digestive
  • endocrine
  • circulatory
  • immune

[expand title=”Show Answer”]Answer:
immune
[/expand]

9. CRISPR-Cas9 was discovered in which of the following organisms?

  • protists
  • fungi
  • bacteria
  • viruses

[expand title=”Show Answer”]Answer:
bacteria
[/expand]

10. Scientists have adapted it to turn it into a biotechnology tool for _ DNA.

  • building
  • editing
  • destroying
  • synthesizing

[expand title=”Show Answer”]Answer:
editing
[/expand]

11. The enzyme portion of this process is called __.

  • CRISPR
  • Replicase
  • Lysozyme
  • Cas9

[expand title=”Show Answer”]Answer:
Cas9
[/expand]

12. What type of RNA is used as a GPS of sorts, to find or locate specific genes?

  • mRNA
  • gRNA
  • tRNA
  • rRNA

[expand title=”Show Answer”]Answer:
gRNA
[/expand]

13. What is PAM?

  • a 3 letter sequence for binding on the target DNA
  • the main gene portion of the DNA
  • the cut site for Cas9
  • a 10 letter sequence for binding on the target DNA

[expand title=”Show Answer”]Answer:
a 3 letter sequence for binding on the target DNA
[/expand]

14. After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following types of repair mechanisms creates a mutation and inactivates the gene.

  • Homology-Directed Repair (HDR edited DNA strand)
  • Non-Homologous End Joining (NHEJ random DNA)

[expand title=”Show Answer”]Answer:
Non-Homologous End Joining (NHEJ random DNA)
[/expand]

15. After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following repair mechanisms involves “editing” of the gene with insertion of a corrected sequence?

  • Homology-Directed Repair (HDR edited DNA strand)
  • Non-Homologous End Joining (NHEJ random DNA)

[expand title=”Show Answer”]Answer:
Homology-Directed Repair (HDR edited DNA strand)
[/expand]

16. What is one of the enzymes produced by the CRISPR system?

  • Cas – 9
  • Cas – 8
  • Cas – 7
  • Cas – 5

[expand title=”Show Answer”]Answer:
Cas – 9
[/expand]

17. What is a Gene?

  • A segment of RNA that encodes for a protein
  • A chromosome
  • A segment of DNA that encodes for a protein
  • Your genome

[expand title=”Show Answer”]Answer:
A segment of DNA that encodes for a protein
[/expand]

18. In order for offspring to get a genetic mutation, the mutation must occur in what type of cell?

  • Cells with no nucleus.
  • Any body cell after birth.
  • Cells with no cell wall or cell membrane.
  • Cells like eggs or sperm cells that are undergoing reproduction.

[expand title=”Show Answer”]Answer:
Cells like eggs or sperm cells that are undergoing reproduction.
[/expand]

19. What is the correct order of steps in the CRISPR process?

  • targeting, binding, cleaving, repairing
  • targeting, repairing, binding, cleaving
  • binding, targeting, repairing, cleaving,
  • targeting, cleaving, repairing, binding

[expand title=”Show Answer”]Answer:
targeting, binding, cleaving, repairing
[/expand]

Citation

APA

MN Editors. (August 20, 2022).MCQ on CRISPR. Retrieved from https://microbiologynote.com/mcq-on-crispr/

MLA

MN Editors. "MCQ on CRISPR." Microbiology Note, Microbiologynote.com, August 20, 2022.

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