Microbiology MCQ – MCQ on Structure of DNA
1. A peculiar cytochrome is observed in bacteria and it can react with molecular oxygen, what is it?
- Cyt b
- Cyt c
- Cyt d
- Cyt o
[expand title=”Show Answer”]Answer: d
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2. The genetic material in HIV is
- ds DNA
- ss DNA
- s RNA
- None of these
[expand title=”Show Answer”]Answer: a
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3. Which one of the following mutagens act only on replicating DNA?
- Ethidium bromide
- Nitrosogeranidine
- Acridine orange
- None of above
[expand title=”Show Answer”]Answer: c
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4. Genomic DNA is extracted, broken into fragments of reasonable size by a restriction endonuclease and then inserted into a cloning vector to generate chimeric vectors. The cloned fragments are called
- Clones
- Genomic library
- mRNA
- None of these
[expand title=”Show Answer”]Answer: b
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5. Transgenic animals are produced when GH gene fused with
- MT gene
- GH
- GRF
- FIX
[expand title=”Show Answer”]Answer: a
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6. In which medium the hydridoma cells grow selectively?
- Polyethylene glycol
- Hypoxanthine aminopterin thyminine
- Hypoxathing-guaning phosphoribosyl transferase
- Both b and c
[expand title=”Show Answer”]Answer: b
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7. The enzymes which are commonly used in genetic engineering are
- Exonuclease and ligase
- Restriction endonuclease and polymerase
- Ligase and polymerase
- Restriction endonuclease and ligase
[expand title=”Show Answer”]Answer: a
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8. A successful hybridoma was produced by fusing
- Plasma cells and plasmids
- Plasma cells and myeloma cells
- Myeloma cells and plasmids
- Plasma cells and bacterial cells
[expand title=”Show Answer”]Answer: b
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9. Poly A tail is frequently found in
- Histone in RNA
- Bacterial RNA
- eukaryotic RNA
- TRNA
[expand title=”Show Answer”]Answer: c
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10. Which of the following is an example of RNA virus?
- SV 40
- T4 phage
- Tobacco mosaic virus
- Adeno virus
[expand title=”Show Answer”]Answer: c
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11. The technique involved in comparing the DNA components of two samples is known as
- Monoclonal antibody techniques
- Genetic finger printing
- Recombinant DNA technology
- Polymerase chain reaction
[expand title=”Show Answer”]Answer: b
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12. Plasmids are ideal vectors for gene cloning as
- They can be multiplied by culturing
- They can be multiplied in the laboratory using enzymes
- They can replicate freely outside the bacterial cell
- They are self replicating within the bacterial cell
[expand title=”Show Answer”]Answer: d
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13. Humans normally have 46 chromosomes in skin cells. How many autosomes would be expected in a kidney cell?
- 46
- 23
- 47
- 44
[expand title=”Show Answer”]Answer: d
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14. Pasteur effect is due to
- Change from aerobic to anaerobic
- Providing oxygen to anaerobically respiring structures
- Rapid utilization of ATP
- Nonsynthesis of ATP
[expand title=”Show Answer”]Answer: b
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15. A mechanism that can cause a gene to move from one linkage group to another is
- Trans location
- Inversion
- Crossing over
- Duplication
[expand title=”Show Answer”]Answer: a
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16. The smallest unit of genetic material that can undergo mutation is called
- Gene
- Cistron
- Replicon
- Muton
[expand title=”Show Answer”]Answer: d
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17. The two chromatids of metaphase chrosome represent
- Replicated chromosomes to be separated at anaphase
- Homologous chromosomes of a diploid set
- Non-homologous chromosomes joined at the centromere
- Maternal and paternal chromosomes joined at the centromere
[expand title=”Show Answer”]Answer: a
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18. Malate dehydrogenase enzyme is a
- Transferase
- Hydrolase
- Isomerase
- Oxido reductase
[expand title=”Show Answer”]Answer: d
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19. In E.Coli att site is in between
- Gal and biogenes
- Bio and niacin genes
- Gal and B genes
- None of these
[expand title=”Show Answer”]Answer: a
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20. The best vector for gene cloning
- Relaxed control plasmid
- Stringent control plasmid
- Both a and b
- None of these
[expand title=”Show Answer”]Answer: a
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21. A gene that takes part in the synthesis of polypeptide is
- Structural gene
- Regulator gene
- Operator gene
- Promoter gene
[expand title=”Show Answer”]Answer: a
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22. DNA replicates during
- G1 – phase
- S – phase
- G2 – phase
- M – phase
[expand title=”Show Answer”]Answer: b
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23. A human cell containing 22 autosome and a ‘Y’ chromosome is probably a
- Male somatic cell
- Zygote
- Female somatic cell
- Sperm cell
[expand title=”Show Answer”]Answer: b
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24. Crossing-over most commonly occurs during
- Prophase I
- Prophase II
- Anaphase I
- Telophase II
[expand title=”Show Answer”]Answer: a
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25. DNA-replication is by the mechanism of
- Conservative
- Semiconservative
- Dispersive
- None of the above
[expand title=”Show Answer”]Answer: b
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26. Production of RNA from DNA is called
- Translation
- RNA splicing
- Transcription
- Transposition
[expand title=”Show Answer”]Answer: c
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27. Nucleic acids contain
- Alanine
- Adenine
- Lysine
- Arginine
[expand title=”Show Answer”]Answer: b
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28. What are the structural units of nucleic acids?
- N-bases
- Nucleosides
- Nucleotides
- Histones
[expand title=”Show Answer”]Answer: c
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29. The most important function of a gene is to synthesize
- Enzymes
- Hormones
- RNA
- DNA
[expand title=”Show Answer”]Answer: a
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30. One of the genes present exclusively on the X-chromosome in humans is concerned with
- Baldness
- Red-green colour baldness
- Facial hair/moustache in males
- Night blindness
[expand title=”Show Answer”]Answer: b
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