Microbiology MCQ – MCQ on Structure of DNA
1. A peculiar cytochrome is observed in bacteria and it can react with molecular oxygen, what is it?
- Cyt b
- Cyt c
- Cyt d
- Cyt o
2. The genetic material in HIV is
- ds DNA
- ss DNA
- s RNA
- None of these
3. Which one of the following mutagens act only on replicating DNA?
- Ethidium bromide
- Nitrosogeranidine
- Acridine orange
- None of above
4. Genomic DNA is extracted, broken into fragments of reasonable size by a restriction endonuclease and then inserted into a cloning vector to generate chimeric vectors. The cloned fragments are called
- Clones
- Genomic library
- mRNA
- None of these
5. Transgenic animals are produced when GH gene fused with
- MT gene
- GH
- GRF
- FIX
6. In which medium the hydridoma cells grow selectively?
- Polyethylene glycol
- Hypoxanthine aminopterin thyminine
- Hypoxathing-guaning phosphoribosyl transferase
- Both b and c
7. The enzymes which are commonly used in genetic engineering are
- Exonuclease and ligase
- Restriction endonuclease and polymerase
- Ligase and polymerase
- Restriction endonuclease and ligase
8. A successful hybridoma was produced by fusing
- Plasma cells and plasmids
- Plasma cells and myeloma cells
- Myeloma cells and plasmids
- Plasma cells and bacterial cells
9. Poly A tail is frequently found in
- Histone in RNA
- Bacterial RNA
- eukaryotic RNA
- TRNA
10. Which of the following is an example of RNA virus?
- SV 40
- T4 phage
- Tobacco mosaic virus
- Adeno virus
11. The technique involved in comparing the DNA components of two samples is known as
- Monoclonal antibody techniques
- Genetic finger printing
- Recombinant DNA technology
- Polymerase chain reaction
12. Plasmids are ideal vectors for gene cloning as
- They can be multiplied by culturing
- They can be multiplied in the laboratory using enzymes
- They can replicate freely outside the bacterial cell
- They are self replicating within the bacterial cell
13. Humans normally have 46 chromosomes in skin cells. How many autosomes would be expected in a kidney cell?
- 46
- 23
- 47
- 44
14. Pasteur effect is due to
- Change from aerobic to anaerobic
- Providing oxygen to anaerobically respiring structures
- Rapid utilization of ATP
- Nonsynthesis of ATP
15. A mechanism that can cause a gene to move from one linkage group to another is
- Trans location
- Inversion
- Crossing over
- Duplication
16. The smallest unit of genetic material that can undergo mutation is called
- Gene
- Cistron
- Replicon
- Muton
17. The two chromatids of metaphase chrosome represent
- Replicated chromosomes to be separated at anaphase
- Homologous chromosomes of a diploid set
- Non-homologous chromosomes joined at the centromere
- Maternal and paternal chromosomes joined at the centromere
18. Malate dehydrogenase enzyme is a
- Transferase
- Hydrolase
- Isomerase
- Oxido reductase
19. In E.Coli att site is in between
- Gal and biogenes
- Bio and niacin genes
- Gal and B genes
- None of these
20. The best vector for gene cloning
- Relaxed control plasmid
- Stringent control plasmid
- Both a and b
- None of these
21. A gene that takes part in the synthesis of polypeptide is
- Structural gene
- Regulator gene
- Operator gene
- Promoter gene
22. DNA replicates during
- G1 – phase
- S – phase
- G2 – phase
- M – phase
23. A human cell containing 22 autosome and a ‘Y’ chromosome is probably a
- Male somatic cell
- Zygote
- Female somatic cell
- Sperm cell
24. Crossing-over most commonly occurs during
- Prophase I
- Prophase II
- Anaphase I
- Telophase II
25. DNA-replication is by the mechanism of
- Conservative
- Semiconservative
- Dispersive
- None of the above
26. Production of RNA from DNA is called
- Translation
- RNA splicing
- Transcription
- Transposition
27. Nucleic acids contain
- Alanine
- Adenine
- Lysine
- Arginine
28. What are the structural units of nucleic acids?
- N-bases
- Nucleosides
- Nucleotides
- Histones
29. The most important function of a gene is to synthesize
- Enzymes
- Hormones
- RNA
- DNA
30. One of the genes present exclusively on the X-chromosome in humans is concerned with
- Baldness
- Red-green colour baldness
- Facial hair/moustache in males
- Night blindness
