MCQ on Structure of DNA and RNA

MN Editors

Microbiology MCQ – MCQ on Structure of DNA

1. A peculiar cytochrome is observed in bacteria and it can react with molecular oxygen, what is it?

  • Cyt b
  • Cyt c
  • Cyt d
  • Cyt o

2. The genetic material in HIV is

  • ds DNA
  • ss DNA
  • s RNA
  • None of these

3. Which one of the following mutagens act only on replicating DNA?

  • Ethidium bromide
  • Nitrosogeranidine
  • Acridine orange
  • None of above

4. Genomic DNA is extracted, broken into fragments of reasonable size by a restriction endonuclease and then inserted into a cloning vector to generate chimeric vectors. The cloned fragments are called

  • Clones
  • Genomic library
  • mRNA
  • None of these

5. Transgenic animals are produced when GH gene fused with

  • MT gene
  • GH
  • GRF
  • FIX

6. In which medium the hydridoma cells grow selectively?

  • Polyethylene glycol
  • Hypoxanthine aminopterin thyminine
  • Hypoxathing-guaning phosphoribosyl transferase
  • Both b and c

7. The enzymes which are commonly used in genetic engineering are

  • Exonuclease and ligase
  • Restriction endonuclease and polymerase
  • Ligase and polymerase
  • Restriction endonuclease and ligase

8. A successful hybridoma was produced by fusing

  • Plasma cells and plasmids
  • Plasma cells and myeloma cells
  • Myeloma cells and plasmids
  • Plasma cells and bacterial cells

9. Poly A tail is frequently found in

  • Histone in RNA
  • Bacterial RNA
  • eukaryotic RNA
  • TRNA

10. Which of the following is an example of RNA virus?

  • SV 40
  • T4 phage
  • Tobacco mosaic virus
  • Adeno virus

11. The technique involved in comparing the DNA components of two samples is known as

  • Monoclonal antibody techniques
  • Genetic finger printing
  • Recombinant DNA technology
  • Polymerase chain reaction

12. Plasmids are ideal vectors for gene cloning as

  • They can be multiplied by culturing
  • They can be multiplied in the laboratory using enzymes
  • They can replicate freely outside the bacterial cell
  • They are self replicating within the bacterial cell

13. Humans normally have 46 chromosomes in skin cells. How many autosomes would be expected in a kidney cell?

  • 46
  • 23
  • 47
  • 44

14. Pasteur effect is due to

  • Change from aerobic to anaerobic
  • Providing oxygen to anaerobically respiring structures
  • Rapid utilization of ATP
  • Nonsynthesis of ATP

15. A mechanism that can cause a gene to move from one linkage group to another is

  • Trans location
  • Inversion
  • Crossing over
  • Duplication

16. The smallest unit of genetic material that can undergo mutation is called

  • Gene
  • Cistron
  • Replicon
  • Muton

17. The two chromatids of metaphase chrosome represent

  • Replicated chromosomes to be separated at anaphase
  • Homologous chromosomes of a diploid set
  • Non-homologous chromosomes joined at the centromere
  • Maternal and paternal chromosomes joined at the centromere

18. Malate dehydrogenase enzyme is a

  • Transferase
  • Hydrolase
  • Isomerase
  • Oxido reductase

19. In E.Coli att site is in between

  • Gal and biogenes
  • Bio and niacin genes
  • Gal and B genes
  • None of these

20. The best vector for gene cloning

  • Relaxed control plasmid
  • Stringent control plasmid
  • Both a and b
  • None of these

21. A gene that takes part in the synthesis of polypeptide is

  • Structural gene
  • Regulator gene
  • Operator gene
  • Promoter gene

22. DNA replicates during

  • G1 – phase
  • S – phase
  • G2 – phase
  • M – phase

23. A human cell containing 22 autosome and a ‘Y’ chromosome is probably a

  • Male somatic cell
  • Zygote
  • Female somatic cell
  • Sperm cell

24. Crossing-over most commonly occurs during

25. DNA-replication is by the mechanism of

  • Conservative
  • Semiconservative
  • Dispersive
  • None of the above

26. Production of RNA from DNA is called

  • Translation
  • RNA splicing
  • Transcription
  • Transposition

27. Nucleic acids contain

  • Alanine
  • Adenine
  • Lysine
  • Arginine

28. What are the structural units of nucleic acids?

  • N-bases
  • Nucleosides
  • Nucleotides
  • Histones

29. The most important function of a gene is to synthesize

  • Enzymes
  • Hormones
  • RNA
  • DNA

30. One of the genes present exclusively on the X-chromosome in humans is concerned with

  • Baldness
  • Red-green colour baldness
  • Facial hair/moustache in males
  • Night blindness

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