Get Study notes related to practicals of microbiology.
What is Nitrate Reduction Test? Nitrate reduction test is used to differentiate Enterobacteriaceae based on their ability to produce nitrate reductase enzyme, which hydrolyzes nitrate (NO3–) to nitrite (NO2–), which may be further degraded to various nitrogen products such as nitrogen oxide, nitrous oxide, and ammonia (NH3), depending on the organism’s enzyme system and the … Read more
What is the Litmus Milk Test? Litmus Milk Medium is a liquid medium suggested for use in qualitative tests for determining the activity of bacteria on milk and maintaining lactic acid bacteria. The Litmus Milk Test requires a litmus milk medium, which is a liquid medium that differentiates bacteria based on numerous metabolic events in … Read more
What is API (Analytical Profile Index) 20E Test? Principle of APT Test for Bacteria API 20E Test Kit API 20E is a specialized kit designed for bacterial identification, consisting of 20 distinct tests. These tests aid in characterizing various metabolic activities and enzymatic reactions of the organisms being examined. Each test provides valuable information to … Read more
Test Name Acetamide utilization Test Purpose Differentiate microorganisms according to their capacity to utilise acetamide as their only carbon source. Microorganisms Tested Isolated colonies of Gram-negative, non-glucose-fermenting rods that emit a luminous pigment and are suggestive of P. aeruginosa. Result Positive: The slant shows growth, and its colour changes from green to intense blue.Negative: There … Read more
Test Name Acetate Utilization Test Detection Differentiate species based on ability to utilise acetate as the sole source of carbon. Typically used to distinguish Shigella spp. from Escherichia coli. Test organism It is best to use an acetate utilisation test to tell Shigella spp. from Escherichia coli. Test isolates are Gram-negative rods that don’t ferment … Read more
Overview of Butyrate Test Test Name Butyrate Disk Test Detection Enzyme butyrate esterase Uses This is a quick way to find out if Moraxella catarrhalis has the enzyme butyrate esterase. Result A blue colour development Require Bromochloro-indolyl butyrate and 4-methylumbelliferyl impregnated discs Test Organism Moraxella catarrhalis ATCC 25240: butyrate positive Butyrate Disk is a reagent-coated … Read more
Test Name Casein Hydrolysis Test Detection Enzyme caseinase producing microorganisms Uses To show that some microorganisms can make enzymes outside of their cells that can break down the milk protein casein. Result A clear, transparent zone can be seen around the area where the colonies are growing, or sometimes under the area where the colonies … Read more
Cetrimide Selective Agar is a solid medium that is recommended for use in qualitative procedures for selective isolation and presumed identification of Pseudomonas aeruginosa and other nonfermenting, gram-negative bacilli.
Test Name Deoxyribonuclease (DNase) Test Detection DNases enzyme Uses To find out if an organism can break down DNA and use it to get carbon and energy so it can grow.To distinguish Staphylococcus aureus from other Staphylococci. Result DNase Test Agar: After adding 1N HCl, a clear zone forms around the growth line, but the … Read more
Test Name Growth at 42 Test Purpose This test distinguishes pyocyanogenic pseudomonas from other Pseudomonas species.Determine an organism’s capacity to live at a temperature of 42°C.To distinguish fermentative bacteria from non-fermentative bacteria. Uses The test is used to determine an organism’s capacity to grow at 42°C.This test distinguishes pyocyanogenic pseudomonas from other Pseudomonas species. Result … Read more
What is Bile Solubility Test? Definition of Bile Solubility Test The bile solubility test is a biochemical assay used to differentiate Streptococcus pneumoniae from other alpha-hemolytic Streptococci based on the lysis of bacterial cells in the presence of specific bile salts. Objectives of Bile Solubility Test In essence, the bile solubility test is a pivotal … Read more
Leucine aminopeptidase (LAP) is an enzyme used to distinguish catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group, such as Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive).
The pancreas secretes an enzyme called lipase that aids in the digestion of fatty foods. High levels of lipase in the blood may indicate pancreatitis, a disorder characterised by the inflammation and enlargement of the pancreas. Other pancreatic difficulties, certain drugs, and diseases and disorders include kidney failure, cancer, and gallbladder and oesophagus troubles can … Read more
A lipid is a high-energy, low-molecular-weight molecule. After being taken in by the cell, they are processed via aerobic respiration to generate ATP (adenosine triphosphate), the energy currency of the cell (ATP). Other metabolic processes may also use the components to synthesise essential cellular protoplasm. However, degradation is necessary before bacteria can absorb them. Lipases … Read more
The purpose of the malonate test is to see if the organism can get all of its energy from malonate.
The oxidase enzyme is a key factor in distinguishing Staphylococcus from Micrococcus, and the Microdase Disk is a reagent-impregnated disc recommended for use in qualitative procedures.
MUG Disk is a reagent-coated disc that can be used to quickly and probably identify Escherichia coli in qualitative procedures. MUG stands for 4-Methylumbelliferyl-β-D Glucuronide and it acts as a substrate for the organisms that have the enzyme β-glucuronidase. This enzyme is found in 97% of E. coli strains. Salmonella, Shigella, and Yersinia are also … Read more
The novobiocin (antibiotic) differentiation disc can be used in the Novobiocin Susceptibility Test to help make a preliminary distinction between Staphylococcus saprophyticus and other coagulase-negative staphylococci (CoNS) in clinical (urinary) specimens.
Hugh and Leifson published the details of the oxidative-fermentative (OF) test in 1953. “had discovered that some bacteria made acid from carbohydrates solely under aerobic settings and others created acid both under aerobic and anaerobic conditions,” the authors write of previous microbiological research. Fermentation was once characterised as the process by which acid was produced … Read more
The Minimum Inhibitory Concentration (M.I.C.) of antimicrobial drugs against bacteria and the detection of resistance mechanisms can be found with the help of a MIC Test Strip, a quantitative assay.
We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.
We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!
We need money to operate the site, and almost all of it comes from our online advertising.
Please add Microbiologynote.com to your ad blocking whitelist or disable your adblocking software.
