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SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

SSPE (Blot, 20x, pH 7.4) Preparation and Recipe Required components Component Amount Concentration Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) 27.6 g 0.2 M EDTA (mw: 292.24 g/mol) 7.4 g 0.02 M SSPE (Blot, 20x, pH 7.4) Preparation Prepare 800 mL of distilled water in a suitable container. Add 175.3 g of Sodium chloride to the solution. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution. Add 7.4 g of EDTA to the solution. Add distilled water until the volume is 1 L. … Read more

Physiological Buffer preparation

Buffer preparation

Tyrode’s Solution, Acidic Preparation To prepare 1L of Tyrode’s Solution, Acidic, the following components are required; Component Amount Concentration NaCl (mw: 58.44 g/mol) 8 g 0.1368 M KCl (mw: 74.55 g/mol) 200 mg 0.0027 M CaCl2•2H2O (mw: 147.01 g/mol) 240 mg 0.0016 M MgCl2•6H2O (mw: 203.3 g/mol) 100 mg 0.0005 M Glucose (mw: 180.16 g/mol) … Read more

Different Buffer preparation for Gel Electrophoresis

Buffer preparation

Acrylamide Stock Solution Preparation Application: Acrylamide is used along with bisacrylamide to form gels used in SDS-PAGE. Acrylamide forms polyacrylamide polymers, while bisacrylamide crosslinks between polyacrylamide chains. To prepare 1L of Acrylamide Stock Solution the following components are required; Component Amount Concentration acrylamide (mw: 71.08 g/mol) 389.6 g 5.481 M bisacrylamide (mw: 154.17 g/mol) 10.4 … Read more

Solution and Cell/Culture/Growth Media Preparations

Buffer preparation

Chelation Medium Preparation To prepare 1L of Chelation Medium the following components are required. Component Amount Concentration NaCl (mw: 58.44 g/mol) 40.9 g 0.7 M MgCl2·6H2O (mw: 203.3 g/mol) 6.1 g 0.03 M MgSO4·7H2O (mw: 246.47 g/mol) 7.39 g 0.03 M KCl (mw: 74.55 g/mol) 1.49 g 0.02 M EGTA (mw: 380.35 g/mol) 7.6 g … Read more

Carbenicillin Solution Preparation

Buffer preparation

Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against Gram-positive bacteria. Carbenicillin binds and inhibits the enzymes involved in the synthesis of bacterial cell wall. Since carbenicillin is less sensitive to β-lactamases than ampicillin, it is effective against ampicillin-resistant bacteria. It has … Read more

Calcium Chloride Stock Solution Preparation

Buffer preparation

Calcium Chloride Stock Solution Preparation Calcium chloride is a common calcium salt used in a variety of applications. To prepare 1L of Calcium Chloride Stock Solution the following components are required. Component Amount Concentration CaCl2 (mw: 110.98 g/mol) 58 g 0.5226 M Preparation procedure Prepare 800 mL of distilled water in a suitable container. Add … Read more

Bath Solution (Ca2+/Mg2+ Free) Preparation

Buffer preparation

Bath Solution Preparation To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required; Component Amount Concentration KOH (mw: 56.11 g/mol) 7.854 g 0.14 M HEPES (mw: 238.3 g/mol) 4.766 g 0.02 M KCl (mw: 74.55 g/mol) 149 mg 0.002 M EGTA (mw: 380.35 g/mol) 1.902 g 0.005 M Methanesulfonic acid (mw: 96.11 … Read more

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