Staining

Staining

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Wright Stain Principle, Procedure, Result

Wright’s stain is a modified Romanowsky stain. In hematology laboratory, it is used for the staining of peripheral blood...

Jun 28, 2021 · 2 min read >

Nuclear Staining of Filamentous Fungi

Fungi are eukaryotic and contain well-organized nuclei. The fungal nucleus is membrane bound and consist of two membranes of...

Apr 14, 2021 · 1 min read >

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Recommended Books

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Recommended Books for microbiology by expertise. All these books are Recommended for B.Sc and Msc students.

Giemsa Stain: Preparation, Procedure, Principle, Composition and Application

The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain...

Apr 13, 2021 · 4 min read >

Flow Cytometry Laboratory for cell viability staining using propidium iodide.

Flow cytometry is a fast and stable method for the quantification of viable cells. Determining cell viability is a...

Apr 12, 2021 · 2 min read >

Viability Staining Method For Bacteria

Aim To distinguish between living and dead cells. Principle The viability Staining Method mainly used to detect living and...

Apr 12, 2021 · 58 sec read >

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present...

Apr 11, 2021 · 1 min read >

Cell Membrane Staining Procedure, Principle, Result

The cell membrane is a biological membrane which also termed a plasma membrane (PM) or cytoplasmic membrane. It divides...

Apr 11, 2021 · 1 min read >

Cell Wall Staining by Dayr’s Method

The composition of cell wall varies from species to species, it has been reported that the main constituents of...

Apr 10, 2021 · 3 min read >

Collagen Hybridizing Peptide Staining

Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in...

Apr 9, 2021 · 3 min read >

Iron-Hematoxylin Stain Principle, Procedure, Result

Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms...

Apr 7, 2021 · 5 min read >

Acridine Orange Staining

Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular...

Apr 7, 2021 · 1 min read >

Toluidine blue stain of Mast Cell.

Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different...

Apr 6, 2021 · 1 min read >

Sudan Black B Staining

Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids...

Apr 6, 2021 · 1 min read >

Parasporal Crystal Stain Principle, Procedure, Result

Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means...

Apr 5, 2021 · 1 min read >

Bacterial Flagella Staining by Leifson Flagella Stain Method

In 1930, Leifson introduced a simple flagella stain, using this stain he observed that mutations leading to nonflagellated from...

Apr 4, 2021 · 3 min read >

Flagella Staining Principle, Procedure, Result

Flagella can not be seen under a bright-field microscope by using ordinary stains. A simple and useful method has...

Apr 3, 2021 · 2 min read >

Capsule Staining Procedure, Principle, Result

Capsule staining is a differential staining method, where two different stains are used such as the primary stain and...

Apr 3, 2021 · 4 min read >

Endospore Staining Principle, Procedure, Result

There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.

Apr 2, 2021 · 3 min read >

Negative Staining Principle, Procedure, Result

In negative staining method, an acidic dye is used known as India Ink or Nigrosin. When the bacterial cells...

Apr 1, 2021 · 2 min read >
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