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Nitrate Reduction Test Procedure, Principle, Result

Nitrate Reduction Test Procedure, Principle, Result

What is Nitrate Reduction Test? Nitrate reduction test is used to differentiate Enterobacteriaceae based on their ability to produce nitrate reductase enzyme, which hydrolyzes nitrate (NO3–) to nitrite (NO2–),...

Sourav Bio in Biochemical Test Sep 10 · 6 min read

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in Biochemical Test

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Sep 14 · 2 min read

Tollens’ Test Definition, Principle, Procedure, Result, Application.

in Biochemical Test

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Sep 12 · 6 min read

Oxidase Test Definition, Principle, Procedure, Result, Application

in Microbiology Techniques

Coagulase Test Principle, Procedure, Application, Result.

It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).

Aug 19 · 4 min read

Coagulase Test Principle, Procedure, Application, Result.

in Microbiology Techniques

Counter Current Immunoelectrophoresis Principle, Procedure, Result.

Counter current immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody migrate towards opposite directions and form a visible white precipitate in the area between the wells.

Aug 17 · 4 min read

Counter Current Immunoelectrophoresis

in Microbiology Techniques

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.

Aug 16 · 3 min read

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

in Microbiology Techniques

Immunoelectrophoresis test Principle, Applications, Procedure, Results, Advantages and Disadvantages.

Immunoelectrophoresis is a powerful qualitative technique for the characterization of an antibody.

Aug 14 · 4 min read

Immunoelectrophoresis test Principle, Applications, Procedure Results, Advantages and Disadvantages.

in Biochemical Test

Gerhardt’s test Principle, Procedure, Result.

Gerhardt's test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.

Jul 19 · 1 min read

Gerhardt's test

in Hematology

Cross Match Principle, Procedure, Result.

Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.

Jul 5 · 4 min read

Cross Match

in Protocols

Reverse Blood Grouping Principle, Procedure, Result

Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.

Jul 3 · 2 min read

Reverse Blood Grouping

in Protocols

Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Jul 3 · 4 min read

Blood Grouping Principle and Procedure

in Biochemical Test

Reverse CAMP test Principle, Procedure, Result

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Jun 22 · 1 min read

Reverse CAMP test

in Molecular biology

Real Time PCR Principle, Protocol, Application, Advantages.

A real-time polymerase chain reaction (real-time PCR) is a conventional polymerase chain reaction (PCR) based laboratory technique used in molecular biology for real-time monitoring of the amplification of a targeted DNA molecule during the PCR, not at its end, as the conventional PCR do.

May 27 · 7 min read

Real-Time PCR Principle, Protocol, Application, Advantages.

in Protocols

Polymerase Chain Reaction (PCR) Steps, Principle, Application

Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).

May 25 · 6 min read

Polymerase chain reaction (PCR)

in Protocols

Eastern blot Protocol, Principle, Application

A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.

May 23 · 1 min read

Buffer preparation

in Protocols

Northern Blotting Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

May 22 · 8 min read

Northern Blot Protocol, Principle, Application, Result

in Protocols

Western Blot Protocol, Principle, Result.

In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.

May 21 · 7 min read

Western Blot Protocol, Principle, Result

in Protocols

Restriction Digest Protocol, Principle, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.

May 20 · 5 min read

Restriction Digest Principle, Procedure, result

in Protocols

Dot Blot Protocol, Principle, Definition.

Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.

May 18 · 6 min read

Dot Blot Protocol, Principle, Definition

in Microbiology Techniques

Pour Plate Method Principle, Procedure, Objective, Result, Advantages

The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed

Apr 16 · 9 min read

Pour Plate Method Principle, Procedure, Objective, Result

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