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Polymerase Chain Reaction (PCR) Steps, Principle, Application

Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!)...

May 25, 2021 · 7 min read >

Eastern blot Protocol, Principle, Application

A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto...

May 23, 2021 · 1 min read >

Northern Blotting Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of...

May 22, 2021 · 8 min read >

Western Blot Protocol, Principle, Result.

In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This...

May 21, 2021 · 7 min read >

Restriction Digest Protocol, Principle, result

The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition...

May 20, 2021 · 5 min read >

Dot Blot Protocol, Principle, Definition.

Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.

May 18, 2021 · 6 min read >

Pour Plate Method Principle, Procedure, Objective, Result, Advantages

The forerunner of the present pour-plate method was developed in the laboratory of the famous bacteriologist, Robert Koch. In...

Apr 16, 2021 · 3 min read >

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample...

Apr 16, 2021 · 3 min read >

Streak Plate Method: Patterns, Procedure, Principle

Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The...

Apr 15, 2021 · 7 min read >

Giemsa Stain: Preparation, Procedure, Principle, Composition and Application

The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain...

Apr 13, 2021 · 4 min read >

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present...

Apr 11, 2021 · 1 min read >

Cell Membrane Staining Procedure, Principle, Result

The cell membrane is a biological membrane which also termed a plasma membrane (PM) or cytoplasmic membrane. It divides...

Apr 11, 2021 · 1 min read >

Iron-Hematoxylin Stain Principle, Procedure, Result

Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms...

Apr 7, 2021 · 5 min read >

Acridine Orange Staining

Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular...

Apr 7, 2021 · 1 min read >

Capsule Staining Procedure, Principle, Result

Capsule staining is a differential staining method, where two different stains are used such as the primary stain and...

Apr 3, 2021 · 4 min read >

PH Meter Definition, Principle, Parts, Types, Application, Procedure.

pH Meter Definition A pH meter is a precise instrument that weighs the hydrogen-ion movement in water-based suspensions, showing...

Dec 8, 2020 · 8 min read >

Hot air oven Definition, Principle, Parts, Application, Procedure.

Hot air oven Definition A hot air oven is a laboratory instrument that uses dry heat to sterilize laboratory...

Dec 7, 2020 · 4 min read >

BOD Incubator: Definition, Principle, Components, Procedure, Application.

BOD Incubator also known as Biological Oxygen Demand incubator. In microbiology laboratories, it is broadly used for cell culture...

Dec 6, 2020 · 3 min read >

Simmons citrate agar test – Procedure, Principle, Result, Example.

About Simmons citrate agar test Simmons citrate agar test helps in detection of citrate utilizing bacteria. In this test,...

Jun 21, 2020 · 2 min read >
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