Culture Media

Culture Media

Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.

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Brucella Agar Composition, Principle, Preparation, Results, Uses

Brucella is an intracellular parasite which causes epizootic abortions for animals, and septicemic febrile disease or localized infections of...

Jan 23 · 2 min read >

Ashdown’s Agar Composition, Principle, Preparation, Results, Uses

Ashdown's medium can be described as a selective culture medium that allows for the identification and characterisation of Burkholderia...

Jan 23 · 1 min read >

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Recommended Books for microbiology by expertise. All these books are Recommended for B.Sc and Msc students.

VRBA Composition, Principle, Preparation, Results, Uses

Coliforms are all facultative and aerobic rod-shaped, gram-negative, non-spore-forming bacteria that produce lactose by fermentation with gas and acid...

Jan 23 · 2 min read >

Baird Parker Agar Composition, Principle, Preparation, Results, Uses

Baird Parker Agar was created by Baird Parker using the Tellurite–glycine formulation of Zebovitz et al. It allows for...

Jan 23 · 2 min read >

Tryptic Soy Agar Composition, Principle, Preparation, Results, Uses

Tryptic Soy Agar can be used to grow microorganisms. These media are non-selective and general-purpose. They provide enough nutrients...

Jan 23 · 2 min read >

Thioglycollate Broth Composition, Principle, Preparation, Results, Uses

Thioglycolate broth, an enrichment broth that is multipurpose and can be used to determine the oxygen needs of microorganisms,...

Jan 23 · 2 min read >

Amies Transport Medium Composition, Principle, Preparation, Results, Uses

Amies Transport Medium, which contains charcoal to increase the viability and longevity of pathogenic organisms, is an improved transport...

Jan 23 · 1 min read >

Charcoal Selective Medium Composition, Principle, Preparation, Results, Uses

In 1984, Bolton et al. Bolton et al. (1984) suggested that charcoal could be used to replace blood in...

Jan 23 · 1 min read >

Selenite F Broth Composition, Principle, Preparation, Results, Uses

Selenite Broth was invented by Leifson who proved that selenite is inhibitory to bacteria, coliforms, and other species, like...

Jan 23 · 2 min read >

Triple Sugar Iron (TSI) Agar Composition, Principle, Preparation, Results, Uses

The majority of bacteria are able to ferment carbohydrates, especially sugars. Within them, every bacteria is able to ferment...

Jan 23 · 4 min read >

Lysine Iron Agar (LIA) Composition, Principle, Preparation, Results, Uses

Lysine iron Agar (LIA), a differential medium, is used to test organisms' ability to deaminate or decarboxylate Lysine. Lysine...

Jan 23 · 2 min read >

Levinthal’s Medium Composition, Principle, Preparation, Results, Uses

For the cultivation of Haemophilus, Levinthal's Medium can be used. Although there are many species in the genus Haemophilus...

Jan 23 · 1 min read >

EMB Agar Composition, Principle, Preparation, Results, Uses

Eosin Methylene Blue (EMB), a microbiological media, is a differentiating medium that slightly inhibits the growth and color of...

Jan 23 · 2 min read >

YEPD Agar Composition, Principle, Preparation, Results, Uses

Yeasts, unicellular eukaryotes, are a well-studied model organism in molecular genomics. They are chemoorganotrophs because they use organic compounds...

Jan 23 · 1 min read >

BCYE Agar Composition, Principle, Preparation, Results, Uses

In 1978, Feeley et al developed a medium for isolating Legionella species. They later modified it by replacing casein...

Jan 23 · 2 min read >

Egg Yolk Agar Composition, Principle, Preparation, Results, Uses

Egg Yolk Agar modified is based upon the original Egg Yolk Agar formula developed by McClung & Toabe to...

Jan 23 · 2 min read >

Bismuth Sulphite Agar (BSA) Composition, Principle, Preparation, Results, Uses

Salmonellae are the most complex taxonomically diverse group of bacteria in Enterobacteriaceae. Salmonella infections in humans are usually caused...

Jan 23 · 2 min read >

Endo Agar Composition, Principle, Preparation, Results, Uses

Endo created Endo Agar to distinguish gram-negative bacteria based on lactose fermentation and inhibit gram-positive bacteria. The latter were...

Jan 23 · 2 min read >

Middlebrook Agar Composition, Principle, Preparation, Results, Uses

Over the years, many media have been developed for mycobacteria cultivation. Early ones included egg-based formulations such as Lowenstein-Jensen...

Jan 23 · 2 min read >
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