Question

Haemoglobin protein of a normal individual has to be compared with that of a person with sickle cell anaemia in a pathology laboratory. Represent the steps of the technique, which can be used for the same, in the form of a flow chart.

Answer 1

Here's a flow chart representing the steps of a technique that can be used to compare the hemoglobin protein of a normal individual with that of a person with sickle cell anemia:

1. Obtain blood samples from both individuals.
2. Isolate hemoglobin protein from the blood samples.
3. Perform electrophoresis to separate the hemoglobin proteins based on their charge and size.
4. Stain the electrophoresis gel to visualize the separated proteins.
5. Observe the migration pattern of the hemoglobin proteins.
6. Compare the migration pattern of the normal individual's hemoglobin protein with that of the person with sickle cell anemia.
7. Identify any differences in the migration pattern of the hemoglobin proteins.
8. Confirm the presence of sickle cell anemia by other diagnostic tests, such as DNA sequencing or sickling tests.