Spontaneous Generation Theory Definition
The Spontaneous generation theory was referred to as the living cells were originated from nonliving cells. According to this theory, mice came from corn, flies from bovine manure, maggots from rotting meat, and fish from the mud of previously dry lakes.
In the earliest times, people had believed in spontaneous generation theory. But later this theory was proved wrong. Several experiments were conducted to disprove spontaneous generation.
In the 19th century, Louis Pasteur’s experiment and other experiments refuted this theory and supported biogenesis.
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Experiments on Spontaneous Generation Theory
Aristotle
The Greek philosopher Aristotle(384–322 BC) supports the spontaneous generation theory. He thought that life was originated from nonliving materials if the material contained pneuma (“vital heat”).
Redi’s Experiment

In 1668, an Italian scientist Francesco Redi conduct an experiment on a piece of fresh meat to disprove the spontaneous theory.
- Redi placed a piece of fresh meat in tow different jars.
- He left one jar open and the second one was covered with a cloth.
- After a few days, he observed that the open jar contained maggots whereas the covered jar contained no maggots.
- He also noticed that maggots were found on the exterior surface of the cloth, which was used to cover the jar.
From this experiment, Redi proved that maggots came from the fly eggs means spontaneous generation theory was wrong. Because according to this theory living cells were originated from the nonliving cells.
John Needham’s Experiment
Later, An England scientist, John Needham proved Redi’s experiment wrong by placing broth or gravy in a bottle and heated the bottle to kill the indie’s germs, then he sealed it. After a few days, he found life inside the bottle and announced that life is originated from nonliving cells ( In actuality, he did not heat it long enough to kill all the microbes).
Spallanzani’s Experiment
An Italian scientist, Lazzaro Spallanzani combined both experiments of Needham and Redi and constructed his own experiment.
- Spallanzani conducted her experiment by placing broth in two different bottles.
- Then he boiled both broth inside the bottle.
- Then he sealed on the bottle and left open the second one.
- After a few days, he observed that the open bottle contains life was as the sealed bottle contain no life.
- Although his experiment was successful except it was noted by scientists of the day that Spallanzani had deprived the closed bottle of air, and it was thought that air was necessary for spontaneous generation. a strong rebuttal blunted his claims.
Pasteur’s Experiment

The French scientist, Louis Pasteur accept the challenge and conduct his experiment on spontaneous generation.
- He designed several bottles with S-curved necks or also known as gooseneck flask, which were oriented downward so gravity would prevent access by airborne foreign materials.
- Then he placed nutrient-enriched broth in one goose-neck bottle.
- Then he boiled the broth inside the bottle and kept it for one year in sealed condition.
- After one year he observed no life in the jar.
- Then he broke the top portion of the goose-neck bottle and directly exposed to the air.
- After a few days, he observed that the broth has contains life.
- He noticed that no life forms were observed in the broth until this obstacle was removed from the bottle, because air born particles and dust were trapped in the S-shaped neck of the bottle.
- He confirmed that the contamination came from life-forms in the air. From this experiment, he disproves the spontaneous generation theory.
John Tyndall
- In 1876, an English physicist John conduct an experiment to support the Pasteur’s experiment.
- He designed an apparatus to prove that air carries particulate matter.
- From his experiment, he proved that pure air is free of microorganisms. When this pure air is introduced into life-supporting media no microorganism was formed.
Biogenesis Definition
- Biogenesis is referred to as the living cells are originated from the nonliving cells.
- Ther term biogenesis also refers to biochemical processes of production in living organisms.
- The biogenesis theory was proved by Louis Pasteur, that living things come only from other living things life does not spontaneously arise from non-living material.
- An English physiologist and neurologistHenry, Charlton Bastian first coined the term biogenesis.
- An English biologist and anthropologist Thomas Henry Huxley first coined the term abiogenesis.
Questions On Spontaneous Generation
1. What is spontaneous generation and who disproved the theory?
The Spontaneous generation theory was referred to as the living cells were originated from nonliving cells. According to this theory, mice came from corn, flies from bovine manure, maggots from rotting meat, and fish from the mud of previously dry lakes.
Francesco Redi, Lazzaro Spallanzani, Louis Pasteur, John Tyndall disproved the theory.
2. Are abiogenesis and spontaneous generation the same?
Yes both the terms are same. An English biologist and anthropologist Thomas Henry Huxley first coined the term abiogenesis.
3. Who proposed the theory of spontaneous generation?
Spontaneous generation theory was first proposed by Aristotle (384–322 BC).
4. What is meant by the idea of spontaneous generation?
Spontaneous generation means, living cells were originated from nonliving materials.
5. What is an example of a spontaneous generation?
Follow Redi’s Experiment.
6. Does spontaneous generation occur?
Yes, spontaneous generation occurs.
Then write Redi’s Experiment
7. Is spontaneous generation true?
Yes, spontaneous generation occur.
Then write Redi’s Experiment
8. Why was spontaneous generation believed for so long?
Write the whole portion of “Experiments on Spontaneous Generation Theory“
9. Does meat spontaneously generate flies and maggots?
No. Follow Redi’s Experiment
10. Who disproved spontaneous generation?
Louis Pasteur and John Tyndall in the mid-19th century.
11. How long was spontaneous generation believed true?
Belief in spontaneous generation lasted until the 1860s, when Louis Pasteur’s experiments brought germ theory to the world
12. Why was the theory of spontaneous generation rejected?
Follow John Needham’s Experiment and last point of Spallanzani’s Experiment.
13. What is spontaneous generation history?
Write this whole article.
14. Is spontaneous generation correct or incorrect?
Follow Redi’s experiment.
15. Can living matter come from nonliving matter?
No.
16. What was the variable in Redi’s experiment?
The independent (manipulated) variable was the gauze. follow the article for more detail
17. What was REDI’s initial observation?
His initial observation was living cells were generated from living material.
18. Did Louis Pasteur believe spontaneous generation?
No.
19. What did Redi conclude?
His initial observation was living cells were generated from living material.
20. Did John Needham believe in spontaneous generation?
Yes.
21. Did Lazzaro Spallanzani believe spontaneous generation?
No.
22. What is the swan neck flask experiment?
Follow Pasteur’s Experiment
23. What was Lazzaro Spallanzani experiment?
Follow the experiment of Lazzaro Spallanzani.
24. When did Lazzaro Spallanzani do his experiment?
In 1773
25. What was Francesco Redi’s hypothesis about the appearance of maggots?
follow the experiment.
Reference
- https://www.infoplease.com/math-science/biology/genetics-evolution/origin-of-life-spontaneous-generation
- https://en.wikipedia.org/wiki/Swan_neck_flask
- https://en.wikipedia.org/wiki/Spontaneous_generation
- https://en.wikipedia.org/wiki/Biogenesis
- https://www.biologyonline.com/dictionary/biogenesis