Culture Media

Czapek’s Agar (CZA) Composition, Principle, Preparation, Results, Uses

Czapek medium (also known as Czapek’s agar or Czapek Dox medium) is a medium used to grow fungi and other organisms. This...

MN Editors avatar
MN Editors
This article writter by MN Editors on January 21, 2022

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

· 1 min read >
Czapek’s Agar (CZA) Composition, Principle, Preparation, Results, Uses
Czapek’s Agar (CZA) Composition, Principle, Preparation, Results, Uses

Czapek medium (also known as Czapek’s agar or Czapek Dox medium) is a medium used to grow fungi and other organisms. This medium is suitable for qualitative cultivation of soil bacteria and saprophytic fungi. Czapek originally created the medium in 1902 to cultivate saprophytic mushrooms. Czapek-Dox Agar, a modified formula of the Czapek (1902-1903) and Dox (1910) formulas, is prepared according to Thom & Church. Medium contains sucrose as the only source of carbon, and nitrate is the only inorganic nitrogen source.

Principle of Czapek’s Agar (CZA)

Czapek Medium, a neutral, solid medium that contains only sodium nitrate, is the sole source for nitrogen. This inorganic nitrogen source can be used by bacteria and fungi. Sucrose is a source of energy and the only source of carbon. Dipotassiumphosphate acts as a buffering agent. Potassium chloride has essential ions. Magnesium sulfate, ferrous sulfate, and potassium chloride are all sources of cations.

Composition of Czapek’s Agar (CZA)

IngredientsGms/liter
Sucrose30.000
Sodium nitrate2.000
Dipotassium phosphate1.000
Magnesium sulfate0.500
Potassium chloride0.500
Ferrous sulfate0.010
Agar15.000

Final pH (at 25°C) 7.3±0.2

Preparation and Method of Use of Czapek’s Agar (CZA)

  1. In 1000 ml of distilled water, you can suspend 49.01 grams
  2. To dissolve the medium completely, heat to boiling
  3. For 15 minutes, sterilize by using an autoclave at 15 lbs pressure (121degC).
  4. Keep it at 45-50 degrees Celsius
  5. Mix everything together and pour onto sterilized Petri dishes.
  6. You can inoculate the material by spreading thinly the sample on top of the agar medium.
  7. For 1 to 2 weeks, incubate aerobically at 25-30degC.
  8. Regularly examine the plate for signs of growth.
  9. Each colony type should be subcultured to the appropriate media for isolation. Following established laboratory procedures, identify each colony type.

Pour Tube: Heat the pour tube in a saucepan of boiling water and let it cool to 45-50°C. Mix the ingredients and pour into a sterilized petri dish. Follow the instructions.

Result Interpretation of Czapek’s Agar (CZA)

OrganismsGrowth
Aspergillus brasiliensisGood growth
Candida albicansGood growth; cream-colored colonies
Saccharomyces cerevisiaeGood growth
Aspergillus nigerWhite/yellow mycelium, black spores

Uses of Czapek’s Agar (CZA)

  • APHA recommends Czapek Dox Agar for isolating Aspergillus and Penicillium.
  • Standard Methods of Examination of Water and Wastewater recommends Czapek Medium for the isolation of Aspergillus and Penicillium.
  • This medium is used extensively for taxonomic studies on Penicillium.
  • Czapek Agar is able to grow nearly all saprophytic aspergilli vigorously and produces distinctive mycelia and conidia.
  • For the cultivation of acidophilic organisms like yeasts, the acidity of the medium can be increased.
  • This medium can be used in a wide range of microbiological procedures including soil microbiology testing and fungi and mildew resistance testing.

Limitations of Czapek’s Agar (CZA)

  • This medium is general-purpose and does not support fastidious organisms.
  • For complete identification, it is recommended that colonies grown from pure culture be subject to biochemical, immunological and molecular testing.
close

Don't miss a post

Sign up to receive latest post notification

We don’t spam! Read our privacy policy for more info.

microbiology note app
microbiology note app qr code Scane to download
Download Microbiology Note App Download this app for free from google play store and read ads free notes
Need a Note? Request us
Request

Leave a Reply

Your email address will not be published.

1
0

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

More From Microbiology

Salt Meat Broth

Salt Meat Broth

MN Editors in Culture Media
· 1 min read
Join the Newsletter

Subscribe to our weekly newsletter below and never miss the latest articles.

Ads Blocker Image Powered by Code Help Pro

Ads Blocker Detected!!!

We have detected that you are using extensions to block ads. Please support us by disabling these ads blocker.

Powered By
CHP Adblock Detector Plugin | Codehelppro