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SDS-PAGE – Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (PAGE)

SDS-PAGE - Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (PAGE)

SDS-PAGE (Sodium dodecyl Sulfate) polyacrylamide gel electrophoresis is one of the methods employed in genetics, biochemistry and molecular biology for the separation of proteins based on the molecular mass of their proteins. Proteins’ electrophoretic mobility is dependent on their size. The goal of SDS-PAGE is the separation of proteins based on their dimensions.

Native Polyacrylamide Gel Electrophoresis (PAGE)

Native Polyacrylamide Gel Electrophoresis (PAGE)

Electrophoresis with agarose or other polyacrylamide gels is an established method to separate the biopolymers, determine their identity and purify them because both of these gels have a porous the natural world. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking agent, usually N,N’-methylenebisacrylamide.

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