In this article we will learn 12 important difference between Darkfield and bright Field Microscope.
Definition of Bright Field Microscope
The Bright Field Microscope is a conventional Microscope, in this microscope, the specimen appears as dark against a bright background.
Definition of Dark Field Microscope
The darkfield microscope is a conventional microscope, in this microscope, the specimen appears as bright against a dark background.
Difference Between Bright Field Microscope and Dark Field Microscope
|Topic||Bright Field Microscope||Dark Field Microscope|
|Image Type||Bright field microscope formed a dark image against bright field.||Darkfield Microscope formed a bright image against dark background|
|Specimen Types||Stained, fixed, and live specimens are observed.||We can see the living and unstained cells.|
|Image Formation||In a bright-field microscope, only the scattered lights are able to enter the objective lens and transmitted lights or unscattered light rays are omitted, that’s why the viewer sees a dark image against the brightfield.||The only light that has been reflected or reflected by the specimen forms an image.|
|Specimen Color||Appear as dark or blue (depends on the stain color)||Appears as bright|
|Specimen Background color||Appear as bright||Appear as dark.|
|Type of condenser uses||1. Abbe Condenser |
2. Variable focus Condenser.
3. Achromatic condenser
|1. Abbe Condenser.|
2. Paraboloid condenser.
3. Cardioid Condenser.
|Specimen Structure||It shows us the morphological as well as the internal structure of the specimen.||It show us External structure of specimen in great detail.|
|Expensive||Bright fields are inexpensive||Dark fields are expensive.|
|Specimen Preparation||Specimen preparation or staining is a complex and lengthy process. Takes too much time.||No need stain.|
|Usability||It is easy to use.||operating procedure is not easy as compared to bright field microscope.|
|Study on Metal and Minerals||This microscope does not allow us to observe the Metal and Minerals||Dark-field microscope can use to study for minerals and crystals, thin polymers, and some ceramics.|
Bright Field Image
Dark Field Image