In this article we will learn 12 important difference between Darkfield and bright Field Microscope.
Definition of Bright Field Microscope
The Bright Field Microscope is a conventional Microscope, in this microscope, the specimen appears as dark against a bright background.
Definition of Dark Field Microscope
The darkfield microscope is a conventional microscope, in this microscope, the specimen appears as bright against a dark background.
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Difference Between Bright Field Microscope and Dark Field Microscope
| Topic | Bright Field Microscope | Dark Field Microscope |
| Image Type | Bright field microscope formed a dark image against bright field. | Darkfield Microscope formed a bright image against dark background |
| Specimen Types | Stained, fixed, and live specimens are observed. | We can see the living and unstained cells. |
| Image Formation | In a bright-field microscope, only the scattered lights are able to enter the objective lens and transmitted lights or unscattered light rays are omitted, that’s why the viewer sees a dark image against the brightfield. | The only light that has been reflected or reflected by the specimen forms an image. |
| Specimen Color | Appear as dark or blue (depends on the stain color) | Appears as bright |
| Specimen Background color | Appear as bright | Appear as dark. |
| Type of condenser uses | 1. Abbe Condenser 2. Variable focus Condenser. 3. Achromatic condenser | 1. Abbe Condenser. 2. Paraboloid condenser. 3. Cardioid Condenser. |
| Specimen Structure | It shows us the morphological as well as the internal structure of the specimen. | It show us External structure of specimen in great detail. |
| Expensive | Bright fields are inexpensive | Dark fields are expensive. |
| Specimen Preparation | Specimen preparation or staining is a complex and lengthy process. Takes too much time. | No need stain. |
| Opaque disc | Absent | Present |
| Usability | It is easy to use. | operating procedure is not easy as compared to bright field microscope. |
| Study on Metal and Minerals | This microscope does not allow us to observe the Metal and Minerals | Dark-field microscope can use to study for minerals and crystals, thin polymers, and some ceramics. |
Bright Field Image
Dark Field Image
Reference
- https://microbiologynote.com/darkfield-microscope/
- https://microbiologynote.com/bright-field-microscope-definition-parts-working-principle-application/
- http://www.microbehunter.com/what-are-the-differences-between-brightfield-darkfield-and-phase-contras
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