Differences

14 Difference Between Brightfield and Phase-contrast Microscopy

THIS BLOG INCLUDES: hide 1 14 Difference Between Brightfield and Phase-contrast Microscope 2 References 14 Difference Between Brightfield and Phase-contrast Microscope Topic...

Sourav Bio avatar
Sourav Bio
This article writter by Sourav Bio on July 20, 2020

Writer and Founder of Microbiologynote.com. I am from India and my main purpose is to provide you a strong understanding of Microbiology.

· 49 sec read >
Difference Between Brightfield and Phase-contrast Microscope

14 Difference Between Brightfield and Phase-contrast Microscope

Difference Between Brightfield and Phase-contrast Microscope
TopicBrightfield MicroscopePhase Contrast Microscope
Invented byAntonie van Leeuwenhoek (1632–1724)Frits Zernike (1888-1966) in 1932
Staining The Specimen Must be Stained before their observation under bright field Microscope.Staining is not required.
Changes in refractive indexThis Microscope has no ability to alter the refractive index.A phase microscope can change the refractive index of the specimen.
CondenserThe condenser lens is simple.The condenser is a special type, containing annular stop, producing a hollow cone of light.
Alternative NameAlso knwon as light microscope or simple microscope.Also known as zernike microscope.
Phase-platePhase-plate is not required.Phase-plate is the main component.
Specimen typeNonliving cells are observed Living cells are observed.
CostCheap price.Expensive as compared to Brightfield Microscope
UsabilityIt is easy to use.operating procedure is not easy as compared to bright field microscope.
Image QualityProduces a low-resolution image as compared to Phase Contrast MicroscopeProduces a high-resolution image as compared to Brightfield Microscope
Thick SpecimenDoes not matterThick organisms or specimens can not be observed by this microscope, a distorted image can appear.
Thin SpecimenDoes not matterRequired thin Specimen
cell divisioncell division can’t observe by using this microscope.cell division can be observed by using this microscope.
Intercellular structureNot Visible.Intercellular components of living cells can be observed with high resolution.

Brightfield Microscope Image

Bright Field Microscope

Phase Contrast Microscope Image

Phase Contrast Microscopy

References

Get involved!

Responses

No comments yet
2
0
Writer and Founder of Microbiologynote.com. I am from India and my main purpose is to provide you a strong understanding of Microbiology.

More From Microbiology

Are you excited?Download eBook! Free

Don’t miss the chance, Free download Essentials of Medical Microbiology eBook. 

Join the Newsletter

Subscribe to our weekly newsletter below and never miss the latest articles.