Solution preparation

Ethidium Bromide Solution Preparation and use

Ethidium bromide is used to visualize DNA in agarose gels and in many cesium chloride gradient protocols.

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MN Editors
This article writter by MN Editors on June 01, 2021

Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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Buffer preparation
Buffer preparation

Ethidium Bromide

  • Ethidium bromide is used to visualize DNA in agarose gels and in many cesium chloride gradient protocols. 
  • Ethidium bromide is a toxic chemical and a mutagen. It causes eye and skin irritation.
  • Wear gloves at all times and wash contaminated skin with water. 
  • May be stored indefinitely in a lightproof container at room temperature
  • Ethidium Bromide Appearance: Red to purple powder
  • Molecular formula: C21H20N3Br
  • Molecular weight: 394.3
  • Melting point: 255-266°C with decomposition

Ethidium Bromide Solution use

  • Ethidium bromide is commonly used nucleic acid stain for gel electrophoresis.
  • As a stain to visualize U937 cells to assess cell viability
  • Use to detect polymerase chain reaction products
  • In agarose gel electrophoresis based gel retardation assa.

Components for Ethidium Bromide Solution

The following components are required to prepare 1L of Ethidium Bromide Solution

ComponentAmountConcentration
Ethidium bromide (mw: 394.31 g/mol)10 g0.0254 M

Ethidium Bromide Solution Preparation

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 10 g of Ethidium bromide to the solution.
  3. Add distilled water until volume is 1 L.
  4. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/mL solution to a dark bottle and store at room temperature.

References 

  • “Ethidium Bromide Solution Preparation.” AAT Bioquest, Inc, 26 May. 2021, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ethidium-bromide.
  • https://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006020b.pdf
  • https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/e1385pis.pdf
  • https://www.sigmaaldrich.com/catalog/product/sigma/e1510?lang=en&region=IN
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Microbiology Notes is an educational niche blog related to microbiology (bacteriology, virology, parasitology, mycology, immunology, molecular biology, biochemistry, etc.) and different branches of biology.

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