Lentiviral transfection is a method for introducing genetic material into cells. It involves the use of lentiviruses, a type of retrovirus, to deliver the desired DNA into the target cells. Lentiviral transfection has a unique advantage over other transfection methods, in that it can infect both dividing and non-dividing cells, making it useful for a … Read more
Standard protein expression systems, including E. coli, frequently fail to generate folded, monodisperse, or functional eukaryotic proteins (see Small-scale Expression of Proteins in E. coli). Utilizing a eukaryotic system, such as mammalian cells, that includes the required folding and posttranslational machinery is highly advantageous for the expression of these proteins. Using polyethylenimine, we present strategies … Read more
Principle of Transfection According to the definition of transfection, the external genetic material must enter the cell via the cell membrane. It is essential to highlight that the genetic material, whether DNA or RNA, is negatively charged as a result of the proteins that surround it. Therefore, in an unmanipulated environment, exogenous DNA cannot cross … Read more
Transfection refers to the introduction of foreign DNA (non-host genome genetic material) into a cell. The primary goal of transfection is to modify the host genome in order to express or inhibit the production of the linked protein. The primary purpose of this page is to provide readers with a thorough explanation of the fundamental … Read more
The nucleolus is a structure within a cell’s nucleus that plays a critical role in the life of a cell. This small but mighty organelle is responsible for several important functions, including the synthesis and assembly of ribosomes. In this article, we will explore the nucleolus, its structure and function, and its role in maintaining … Read more
Cell division is a crucial process that allows organisms to grow, repair, and reproduce. One important aspect of cell division is cytokinesis, the process of separating one cell into two daughter cells. While cytokinesis is a universal process, it can vary greatly between different types of cells. In this article, we will compare and contrast … Read more
The study of microorganisms has come a long way since its inception, with advancements in technology and research driving the field of Microbiology forward. The ability to observe and understand microorganisms has given us an insight into the world of tiny organisms that are crucial to life on Earth. The first simple microscope was created … Read more
Herpes simplex viruses (HSVs) are highly host-adapted viruses that can cause a variety of diseases in human hosts. (a) herpes simplex virus type 1 (HSV-1) and (b) herpes simplex virus type 2 (HSV-2) are the two forms of HSVs (HSV-2). Both types share DNA homology, antigenic determinants, tissue tropism, and illness symptoms, but their epidemiology … Read more
Streptococcus pneumoniae Characteristics of Streptococcus pneumoniae Streptococcus pneumoniae, also known as pneumococcus, is a type of bacteria that typically inhabits the upper respiratory tract of humans, including the nose and throat. It can also be found in other parts of the body, such as the lungs and ears. The bacteria can be transmitted through respiratory … Read more
Rabies is a viral disease that causes inflammation of the brain in humans and other mammals. It is transmitted through the saliva of infected animals, usually through a bite. The virus attacks the central nervous system, leading to symptoms such as fever, muscle spasms, and hallucinations. If left untreated, rabies is almost always fatal. It … Read more
Hepatitis D, commonly referred to as “delta hepatitis,” is a viral infection of the liver (HDV). Hepatitis D is only transmitted to individuals who are simultaneously infected with the hepatitis B virus. Hepatitis D is transmitted when blood or other body fluids from an infected individual enter the body of an uninfected person. Hepatitis D … Read more
Hepatitis B is a viral infection that causes liver inflammation, sometimes leading to serious liver damage. The hepatitis B virus (HBV) is transmitted through contact with the blood or other body fluids of an infected person. This can occur through sexual contact, sharing needles, or from an infected mother to her baby at birth. Symptoms … Read more
What is Neisseria Gonorrhoeae? Neisseria gonorrhoeae is a bacterium that can cause the sexually transmitted infection (STI) called gonorrhea. It is transmitted through sexual contact and can infect the genital tract, anus, and throat. Symptoms of gonorrhea can include painful urination, discharge from the genitals, and painful or swollen testicles in men, and abnormal vaginal … Read more
Hepatitis A is a liver inflammation brought on by the hepatitis A virus (HAV). Primarily, the virus is transmitted when an uninfected (and unvaccinated) individual consumes food or water contaminated with the faeces of an infected individual. The disease is strongly linked to unclean water or food, inadequate sanitation, poor personal hygiene, and oral-anogenital contact. … Read more
Hepatitis C is a viral infection that causes liver inflammation, sometimes leading to serious liver damage. The hepatitis C virus (HCV) is spread through contaminated blood, and it can be acute or chronic. Acute hepatitis C is a short-term illness that occurs within the first 6 months after someone is exposed to the virus. Chronic … Read more
Through the bite of an infected Aedes species mosquito (Ae. aegypti or Ae. albopictus), dengue viruses are transmitted to people. These mosquitoes can transmit other viruses including Zika and chikungunya. 4 billion people, or close to half of the world’s population, reside in dengue-risk zones. In risky locations, dengue is frequently the main cause of … Read more
Lassa virus is an RNA virus that is spread to humans through contact with the urine or feces of an infected Mastomys rat. It is a type of virus known as an arenavirus, and it is endemic in West Africa. The virus is named after the town of Lassa, in Borno State, Nigeria, where it … Read more
What is Ebola ? Ebola is a virus that causes a severe and often fatal disease in humans and nonhuman primates (such as monkeys, gorillas, and chimpanzees). The virus is named after the Ebola River in the Democratic Republic of Congo (formerly Zaire), where it was first identified in 1976. Ebola is a viral hemorrhagic … Read more
Crimean-Congo Hemorrhagic Fever (CCHF) is a viral disease that is transmitted to humans by ticks. It is a highly infectious and severe disease that can be fatal in up to 50% of cases. The virus is found in many parts of the world, including Africa, Asia, and Europe. CCHF is caused by a virus in … Read more
Astrovirus is a type of virus that can cause gastroenteritis (also known as stomach flu) in humans. The virus is highly contagious and is primarily spread through close contact with infected individuals or through contaminated food or water. Symptoms of astrovirus infection may include diarrhea, vomiting, abdominal pain, and fever. Most people recover from an … Read more
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