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Filtration Sterilization Types, Mechanism, Uses

Filtration Sterilization Types, Mechanism, Uses

Filtration is the most popular method to sterilize heat sensitive liquid and gases, without exposure to denaturing temperatures. Instead of killing microorganisms that cause contamination the process simply eliminates them. It is the process of choice to sterilize chemical solutions for antibiotics radioisotopes, toxic chemicals or vaccines, as well as carbohydrates, all of which are sensitive to heat.

Sucrose gradient centrifugation

Sucrose gradient centrifugation

Technique for the characterization and preparation of subcellular particles. A sucrose gradient is used to fill a centrifuge tube of the type swinging-bucket design. The bottom is the most dense, and the top the least dense. The solution is then layered with a suspension of the particles. Centrifugation separates particles in the gradient according their density. The centrifugation is over. After that, the bottom of the tube with sucrose particles of equal density drips into a series receiving tubes.

Membrane Filtration Method, Types, Advantages, Disadvantages, Applications.

Membrane Filtration

In this article, we will learn about the Membrane filtration methods, definition, applications, advantages, disadvantages. Before heading towards Membrane Filtration, you have to understand what is filtration? Membrane filtration is a method of separating particles or molecules from a liquid or gas stream using a semi-permeable membrane. The membrane acts as a barrier, allowing some … Read more

Culture medium Definition, Preparation, Classification

Culture medium Definition, Preparation, Classification

The food supply upon which microorganisms thrive in laboratories is termed as a culture medium or culture media. Culture Medium Definition The nutrient preparation on or in which a culture (microorganism) is grown in the laboratory is known as culture medium or culture media. A culture medium is a solid or liquid preparation utilized to … Read more

Carl Woese’s Classification – Three Domain Classification

Carl Woese’s Classification

What is Carl Woese’s Classification? Carl Woese’s Classification Why is rRNA a good ‘Chronometer’? Ribosomal RNA (rRNA) has been recognized as a pivotal molecular tool in the realm of phylogenetic studies and evolutionary biology. Its efficacy as a ‘chronometer’ can be attributed to several intrinsic properties that make it an ideal candidate for tracing evolutionary … Read more

Binomial Nomenclature Definition, Rules, Examples, Advantages, Disadvantages

Binomial Nomenclature

Binomial nomenclature means a system that is used to provide a unique name to organisms, including plants, animals, microorganisms, and other living things. This naming system makes it easier to understand them better and also separates each species from others. Therefore a universally accepted naming system is essential. Binomial nomenclature definition Binomial nomenclature is a … Read more

Direct Microscopic Count (DMC) Advantage, Disadvantage.

Direct Microscopic Count

Direct Microscopic Count (DMC) Direct Microscopic count (DMC) is a quantitative test and used to enumerate the number of bacterial clumps or somatic cells present in milk. This method is also used for the analysis of foods, water, and, in some cases, air for quantitative counting of microorganisms. There are present several steps to accomplish … Read more

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