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Biochemical Test
Hydrogen Sulfide (H2S) Test Principle, Procedure, Result
What is Hydrogen Sulfide (H2S) Test? This test determines whether sulfur-containing chemicals
Biochemical Test
Biochemical tests are laboratory procedures that use specific chemical reactions to identify and characterize microorganisms, such as bacteria. These tests are often used to identify the presence of specific enzymes or metabolic pathways in a microorganism, which can help to distinguish it from other microorganisms.
Some common biochemical tests used in bacteriology include:
- Oxidase test: This test is used to detect the presence of the enzyme cytochrome oxidase, which is involved in the metabolism of oxygen.
- Catalase test: This test is used to detect the presence of the enzyme catalase, which helps to break down hydrogen peroxide.
- Indole test: This test is used to detect the presence of the enzyme tryptophanase, which breaks down the amino acid tryptophan.
- Nitrate reduction test: This test is used to detect the ability of a microorganism to reduce nitrate to nitrite.
- Gelatinase test: This test is used to detect the ability of a microorganism to produce the enzyme gelatinase, which breaks down gelatin.
These are just a few examples of the many different biochemical tests that are used in bacteriology. Biochemical tests can be useful for identifying and classifying bacteria, and for understanding their metabolism and ecological role.
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Biochemical Test
Nitrate Reduction Test Procedure, Principle, Result
What is Nitrate Reduction Test? Nitrate reduction test is used to differentiate
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Litmus Milk Test Principle, Procedure, Result
What is the Litmus Milk Test? Litmus Milk Medium is a liquid
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Biochemical Test
API (Analytical Profile Index) 20E Test Result, Principle, Procedure
What is API (Analytical Profile Index) 20E Test? Principle of APT Test
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Biochemical Test
Acetamide utilization Test Principle, Procedure, result
Test Name Acetamide utilization Test Purpose Differentiate microorganisms according to their capacity
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Biochemical Test
Acetate Utilization Test Principle, Procedure, Results
Test Name Acetate Utilization Test Detection Differentiate species based on ability to
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Biochemical Test
Butyrate Disk Test Principle, Procedure, Result
Overview of Butyrate Test Test Name Butyrate Disk Test Detection Enzyme butyrate
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Casein Hydrolysis Test Principle, Procedure, Result
Test Name Casein Hydrolysis Test Detection Enzyme caseinase producing microorganisms Uses To
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Biochemical Test
Cetrimide Test Principle, Purpose, Procedure, Results
Cetrimide Selective Agar is a solid medium that is recommended for use in qualitative procedures for selective isolation and presumed identification of Pseudomonas aeruginosa and other nonfermenting, gram-negative bacilli.
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Biochemical Test
Deoxyribonuclease (DNase) Test Principle, Procedure, Result
Test Name Deoxyribonuclease (DNase) Test Detection DNases enzyme Uses To find out
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Biochemical Test
Growth at 42 Test – Growth of Non Fermentative Bacteria at 42°C
Test Name Growth at 42 Test Purpose This test distinguishes pyocyanogenic pseudomonas
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Biochemical Test
Bile Solubility Test – Principle, Procedure, Result
What is Bile Solubility Test? Definition of Bile Solubility Test The bile
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Leucine aminopeptidase (LAP) Test Principle, Procedure, Results
Leucine aminopeptidase (LAP) is an enzyme used to distinguish catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group, such as Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive).
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Lipase Test Principle, Procedure, results
The pancreas secretes an enzyme called lipase that aids in the digestion
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Lipid Hydrolysis Test Principle, Procedure, Result
A lipid is a high-energy, low-molecular-weight molecule. After being taken in by
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Biochemical Test
Malonate Test Principle, Procedure, Results
The purpose of the malonate test is to see if the organism can get all of its energy from malonate.
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Microdase Test Principle, Procedure, Result
The oxidase enzyme is a key factor in distinguishing Staphylococcus from Micrococcus, and the Microdase Disk is a reagent-impregnated disc recommended for use in qualitative procedures.
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Biochemical Test
4-Methylumbelliferyl-β-D-Glucuronide (MUG) Test Principle, Procedure, Results
MUG Disk is a reagent-coated disc that can be used to quickly
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Novobiocin Susceptibility Test Principle, Procedure, Results
The novobiocin (antibiotic) differentiation disc can be used in the Novobiocin Susceptibility Test to help make a preliminary distinction between Staphylococcus saprophyticus and other coagulase-negative staphylococci (CoNS) in clinical (urinary) specimens.
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Biochemical Test
OF (Oxidation-Fermentation) Test Principle, Purpose, Procedure
Hugh and Leifson published the details of the oxidative-fermentative (OF) test in
