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Gel Electrophoresis System – Definition, Principle, Parts, Types, Procedure

Gel Electrophoresis System - Definition, Principle, Parts, Types, Procedure

What is Gel Electrophoresis System? Definition of Gel Electrophoresis System Gel electrophoresis is a laboratory technique that uses a gel as a medium to separate and analyze biomacromolecules such as DNA, RNA, and proteins based on their size and charge. An electric field is applied to move the molecules through the gel, with smaller or … Read more

Autoclave – Definition, Principle, Parts, Operating Procedure, Uses

Autoclave

What is an autoclave? Definition of Autoclave An autoclave is a machine that uses steam under pressure to sterilize materials by killing bacteria, viruses, and spores. Who Invented The Autoclave Machine? Autoclave Temperature and Time Pressure Autoclave Pressure and Temperature Chart STERILIZER TEMPERATURE PRESSURE TIME Steam autoclave 121°C (250°F) 15 psi 15 min Unwrapped items … Read more

Alcohol Burner – Definition, Principle, Parts, Uses

Alcohol burner - Definition, Principle, Parts, Uses

What is Alcohol burner? Definition of Alcohol burner An alcohol burner is a small, portable laboratory device that uses denatured alcohol as fuel to produce a controlled flame for heating, sterilization, and experimental purposes. Working Principle of Alcohol burner Factors that affect the working principle of an alcohol burner Several factors can influence the working … Read more

Bunsen Burner – Definition, Principle, Parts, Functions

Bunsen Burner - Definition, Principle, Parts, Functions

What is a Bunsen Burner? Definition of Bunsen Burner A Bunsen burner is a laboratory gas burner that produces a controlled flame for scientific experiments and research purposes. Principle of Bunsen burner The Bunsen burner, a staple in scientific laboratories, operates on foundational principles of combustion and gas mixing. Constructed predominantly from metal, the burner … Read more

Colony Counter – Definition, Types, Parts, Principle

Colony Counter - Definition, Types, Parts, Principle

What is Colony Counter? Definition of Colony Counter A colony counter is a device used in microbiology laboratories to count bacterial or microorganism colonies on a solid growth medium. It simplifies and accelerates the colony counting process, providing an accurate measurement of colony forming units (CFUs) in a given sample. Purpose of colony counting The … Read more

Two Pan or Equal Arm Analytical Balance

Parts of a Two pan or equal arm analytical balance

What is Two pan or equal arm analytical balance? Principle of Two pan or equal arm analytical balance – How does a two-pan or equal-arm analytical balance work? Parts of a Two pan or equal arm analytical balance Additional parts that may be found on some two-pan analytical balances include: While the core components mentioned … Read more

Single Pan or Unequal Arm Mechanical Balance

Single-pan mechanical analytical balance.

What is Single Pan or Unequal Arm Mechanical Balance? Parts of Single Pan/Unequal Arm Mechanical Balance A single pan or unequal arm mechanical balance consists of several key parts that contribute to its functioning and accuracy. Here is a description of the various parts: These parts work together to create a functional and reliable single … Read more

Triple Beam Balance – Parts, Procedure, Uses

Triple Beam Balance - Parts, Procedure, Uses

What is Triple Beam Balance? Definition of Triple Beam Balance A triple beam balance is a precise measuring instrument used to determine the mass of an object. It consists of three beams, each with different sizes and scale readings, allowing for precise measurements ranging from 0.1 grams to 600 grams. Parts of Triple Beam Balance … Read more

Analytical Balance – Diagram, Principle, Parts, Types, Uses

Analytical Balance - Diagram, Principle, Parts, Types, Uses

What is Analytical Balance? Principle of Analytical Balance – Weighing balance principle Types of analytical balance 1. Two pan or equal arm analytical balance 2. Single pan or unequal arm mechanical balance 3. Electronic single pan balance 4. Microbalance Parts of Analytical Balance with Functions Procedure for Operating Analytical Balance – Weighing Procedure of an … Read more

Reversed-Phase Chromatography – Principle, Protocol, Applications

Reversed-Phase Chromatography - Principle, Protocol, Applications

What is Reversed-Phase Chromatography? Principle of Reversed-Phase Chromatography The principle of reversed-phase chromatography (RPC) is rooted in the interaction between molecules possessing hydrophobic groups. In RPC, the stationary phase comprises a solid support material that is modified with both hydrophobic and hydrophilic groups. When a sample is introduced into the chromatographic system, the solvent molecules … Read more

Liquid Chromatography – Definition, Types, Principle, Protocol, Applications

Liquid Chromatography - Definition, Types, Principle, Protocol, Applications

What is Liquid Chromatography? Types of Liquid Chromatography Liquid chromatography can be classified into four main types based on the components involved in the chromatographic process: These different types of liquid chromatography offer versatility in separating and analyzing diverse compounds, catering to various research and industrial applications. Researchers select the appropriate type of liquid chromatography … Read more

Hydrophobic Interaction Chromatography (HIC)

Hydrophobic Interaction Chromatography (HIC)

What is Hydrophobic Interaction Chromatography (HIC)? Principle of Hydrophobic Interaction Chromatography (HIC) The principle of Hydrophobic Interaction Chromatography (HIC) is based on the interactions between the hydrophobic regions of sample protein molecules and a hydrophobic stationary phase or matrix. This principle allows for the separation and purification of proteins based on their hydrophobicity. In HIC, … Read more

Flash chromatography – Principle, Instrumentation, Protocol, Applications

Flash chromatography - Principle, Instrumentation, Protocol, Applications

What is Flash chromatography? Principle of Flash column chromatography The principle of flash chromatography revolves around the rapid movement of the eluent, a liquid, through a short glass column under gas pressure, typically nitrogen or compressed air. The glass column is packed with a stationary phase consisting of an adsorbent material with a defined particle … Read more

Types of Chromatography – Definition, Principle, Steps, Uses

Types of Chromatography - Definition, Principle, Steps, Uses

What is Chromatography? Chromatography Definition Chromatography is a technique used to separate and analyze the components of a mixture based on their properties and interactions with a stationary and mobile phase. What is a stationary phase? What is the mobile phase? Types of Chromatography Here is the Types of Chromatography; Type of Chromatography Description Gas … Read more

Two-Dimensional (Crossed) lmmunoelectrophoresis (2D)

Two-Dimensional (Crossed) lmmunoelectrophoresis

What is Two-Dimensional (Crossed) lmmunoelectrophoresis? Principle of Two-Dimensional (Crossed) lmmunoelectrophoresis The principle of Two-Dimensional (Crossed) Immunoelectrophoresis involves the combination of two distinct electrophoretic steps to separate and identify proteins based on their charge, size, and antigen-antibody interactions. The technique consists of the following steps: The principle of Two-Dimensional Immunoelectrophoresis exploits the migration characteristics of proteins … Read more

Polyacrylamide Gel Electrophoresis (PAGE)

Polyacrylamide Gel Electrophoresis (PAGE)

What is Polyacrylamide Gel Electrophoresis (PAGE)? Definition of Polyacrylamide Gel Electrophoresis (PAGE) Polyacrylamide Gel Electrophoresis (PAGE) is a technique used to separate and analyze proteins or nucleic acids based on their mobility in a porous polyacrylamide gel matrix under the influence of an electric field. Types of Polyacrylamide Gel Electrophoresis (PAGE) There are several types … Read more

SDS-PAGE – Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (PAGE)

SDS-PAGE - Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (PAGE)

SDS-PAGE (Sodium dodecyl Sulfate) polyacrylamide gel electrophoresis is one of the methods employed in genetics, biochemistry and molecular biology for the separation of proteins based on the molecular mass of their proteins. Proteins’ electrophoretic mobility is dependent on their size. The goal of SDS-PAGE is the separation of proteins based on their dimensions.

Mass Spectrometry (MS) – Principle, Parts, Working, Steps, Uses

Mass Spectrometry (MS) - Principle, Parts, Working, Steps, Uses

What is Mass Spectrometry (MS)? Definition of Mass Spectrometry (MS) Mass Spectrometry (MS) is an analytical technique used to identify the chemical composition of a sample by measuring the mass-to-charge ratio of its ions, resulting in a mass spectrum that reveals the molecular and structural information of the sample. Mass Spectrometry Principle Mass Spectrometry operates … Read more

Solid State Fermentation (SSF) – Definition, Steps, Bioreactor, Applications

Solid State Fermentation (SSF)

Solid State Fermentation (SSF) is a process of fermentation utilized by a range of industries such as textile, food, pharmaceuticals and so on. in order to create compounds of microorganisms that use solid supports instead of liquid medium. It’s defined as growing microbes that do not have a flowing aqueous phase that is free-flowing.

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