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Centrifuge – Definition, Principle, Parts, Types

Centrifuge - Definition, Principle, Parts, Types

What is Centrifuge? Definition of Centrifuge A centrifuge is a laboratory instrument that uses spinning and centrifugal force to separate mixtures based on their density. Who first invented the Centrifuge? Overall, the centrifuge is an important tool in a wide range of scientific and industrial applications, as it allows for the efficient separation and purification … Read more

ELISA Plate Reader – Microplate Reader or Assay Reader

ELISA Plate Reader - Microplate Reader or Assay Reader

What is ELISA? What is ELISA reader? Purpose of an ELISA reader Principle of ELISA Plate Reader The principle of an ELISA reader, also known as a microplate reader, revolves around measuring the energy difference in light before and after it passes through a test sample. This is accomplished using a photoelectric colorimeter or spectrophotometer, … Read more

DNA Analyzer – Principle, Parts, Operating, Applications

DNA Analyzer - Principle, Parts, Operating, Applications

DNA Analyzer Principle  Parts of DNA Analyzer Operating DNA Analyzer Calibration and correct setup are crucial for using a DNA analyzer effectively. The DNA analyzer’s calibration and initialization process consists of the following steps: Calibrating a DNA Analyzer The DNA analyzer undergoes both spatial and spectral calibration. Setting Up the Instrument Operating Procedure of DNA … Read more

Double Beam Balance – Principle, Procedure, Purpose, Uses

Double Beam Balance - Principle, Procedure, Purpose, Uses

Double Beam Balance Principle The Double Beam Balance (mechanical balance) is intended for laboratory and classroom use. Based on the basic Roberval balance principle, the Double Beam Balance permits the determination of the mass differential (rather than the absolute value) between two objects. These balances are supplied with sliding masses. Double Beam Scales are renowned … Read more

Laboratory Flask – Definition, Types, Uses

Laboratory Flask - Definition, Types, Uses

What is Laboratory flask? Glassware is the category of laboratory equipment that includes laboratory flasks. In the laboratory and other scientific contexts, flasks are typically referred to as flasks. There are a variety of flask forms and sizes, but one defining feature is a broader vessel “body” and one (or sometimes more) narrower tubular pieces … Read more

Affinity chromatography Principle, Types, Steps, Applications

Affinity Chromatography Definition, Principle, Components, Steps, Applications

Chromatography is a crucial biophysical method that allows an identification, separation and separation of the constituents of a mixture to enable quantitative and qualitative analysis. Chromatography is an separation method that involves causing a mobile phase that is carrying a mixture is made to move into contact with a stationary phase.

Types of Spectroscopy with Definition, Principle, Steps, Uses

Types of Spectroscopy with Definition, Principle, Steps, Uses

It is the science of studying the interactions between light and matter , where the emission and absorption of the light radiations by the material are investigated and assessed. The focus of spectroscopy is the dispersion of light as well as other radiations caused by the object, which allows the examination of the various characteristics that the objects.

X-Ray Crystallography

X-Ray Crystallography

Crystallography using X-rays is a highly effective non-destructive method for determining how molecules form the crystal. The X-ray crystallography technique utilizes the principles of X-ray diffraction in order to examine the specimen, however it’s conducted in a variety of directions, so it is possible that the 3-D structures of the sample can be constructed. It is a method that has been used to determine three-dimensional crystal structures of numerous substances, particularly biological ones.

Electron Spin Resonance (ESR) – Principle, Instrumentation, Applications

Electron Spin Resonance (ESR) Principle, Instrumentation, Applications

Electron Spin Resonance (ESR) commonly referred to by the name Electron Magnetic Resonance (EMR) or Electron Paramagnetic Resonance (EPR) is an absorption spectroscopy where radiation with frequencies within the microwave range (0.04 to 25 cm) is absorption by paramagnetic materials to cause changes in the magnetic levels of electrons having unpaired spins.

20 Tools Used in Microbiology Laboratory

Tools Used in Microbiology Laboratory

The most commonly used equipment is inoculation needles, transfer loops, inoculation, Bunsen burner, autoclave (or pressure cooker) incubators, hot air oven centrifuge, spectrophotometer magnetic stirrer electric shaker and rotary shaker heating plate, heating mantle distillation plant, UV-lamp carbon dioxide cylinder, water-bath and a single-pan balance that has weights (for general use) chemical balance, fine analytical balance pH meters, Quebec colony counter, Laminar air flow, camera lucida electrophoresis and a high-quality microscope and many more. To perform photomicrography, a photomicrographic camera mounted in a microscope equipped with every accessory is essential in the microbiology lab.

Native Polyacrylamide Gel Electrophoresis (PAGE)

Native Polyacrylamide Gel Electrophoresis (PAGE)

Electrophoresis with agarose or other polyacrylamide gels is an established method to separate the biopolymers, determine their identity and purify them because both of these gels have a porous the natural world. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking agent, usually N,N’-methylenebisacrylamide.

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