Get Study notes related to practicals of microbiology.
Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against Gram-positive bacteria. Carbenicillin binds and inhibits the enzymes involved in the synthesis of bacterial cell wall. Since carbenicillin is less sensitive to β-lactamases than ampicillin, it is effective against ampicillin-resistant bacteria. It has … Read more
Calcium Chloride Stock Solution Preparation Calcium chloride is a common calcium salt used in a variety of applications. To prepare 1L of Calcium Chloride Stock Solution the following components are required. Component Amount Concentration CaCl2 (mw: 110.98 g/mol) 58 g 0.5226 M Preparation procedure Prepare 800 mL of distilled water in a suitable container. Add … Read more
Bath Solution Preparation To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required; Component Amount Concentration KOH (mw: 56.11 g/mol) 7.854 g 0.14 M HEPES (mw: 238.3 g/mol) 4.766 g 0.02 M KCl (mw: 74.55 g/mol) 149 mg 0.002 M EGTA (mw: 380.35 g/mol) 1.902 g 0.005 M Methanesulfonic acid (mw: 96.11 … Read more
Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.
This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used as a reagent for calcium in tissue where it forms a scarlet lake.
This “Mayer’s hemalum solution – for microscopy” is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin.
Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.
Ethidium bromide is used to visualize DNA in agarose gels and in many cesium chloride gradient protocols.
Cresol red is used as a tracking dye for DNA/RNA in agarose gel electrophoresis and protein in polyacrylamide gel electrophoresis.
It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development but does not affect the sporozoites.
The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.
Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.
This solution is mainly used to determine the concentration of proteins in solution.
Anesthetic Solution (Buffered MS222) is commonly used to temporarily immobilize fish and amphibians.
5-fluorocytosine (5-FC) also known as Flucytosine. 5-Fluorocytosinewas first synthesized in 1957 as anti-tumor drug. It is an antifungal medication which is used with amphotericin B against Candida infections and cryptococcosis. This drug is mainly taken by mouth and by injection into a vein.
What is a serial dilution? Also Read: MCQ on Serial Dilution Serial dilution definition Serial dilution is a methodical process in which a solution is diluted sequentially to produce a series of progressively reduced concentrations. What is the purpose of serial dilution? – Serial Dilution Objectives Serial dilution serves as a foundational procedure in numerous … Read more
Aim Transfer of microbial cells from a parent culture medium to a fresh broth culture. Principle The broth is a liquid medium, which is used to grown microorganisms within the laboratory on a small scale. It also used in an industrial fermentor on a large scale. The media called broths, milks, or nutrient solutions are … Read more
Overview of Subculturing Technique In microbiology, subculture is defined as a new cell or microbiological culture, which is prepared by transferring a few or all cells to a fresh growth medium from an old culture or a previous culture. In a single word, the transfer of cells from a previous culture to a fresh or … Read more
The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more
Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more
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